Lack of effects on female fertility or pre- and postnatal development of offspring in rats after exposure to AS03-adjuvanted recombinant plant-derived virus-like particle vaccine candidate for COVID-19

Dubé, Charlotte; Paris-Robidas, Sarah; Primakova, Iryna; Destexhe, Éric; Ward, Brian J.; Landry, Nathalie; Trépanier, Sonia

doi:10.1016/j.reprotox.2021.11.006

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…Ergebnisse Im Tiermodell zeigten sich trotz sehr hoher Impfdosen keine negativen Effekte auf die Fertilität, den Schwangerschaftsverlauf und die fetale Entwicklung 1 2 3 . Auf humaner Seite wurde bei infizierten Frauen keine SARS-CoV-2 RNA in Oozyten/Follikelflüssigkeit nachgewiesen 4 5 6 ; ebenso zeigten sich zwischen Genesenen, Geimpften und Kontrollen keine Unterschiede hinsichtlich Schwangerschafts- oder Lebendgeburtenrate sowie der Gesundheit der Kinder (7–11).…”

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SARS-CoV-2-Infektion, Corona-Impfung und Reproduktion

Feil

Braun

Reiser

et al. 2022

Geburtshilfe Und Frauenheilkunde

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SARS-CoV-2-Infektion, Corona-Impfung und Reproduktion

Feil

Braun

Reiser

et al. 2022

Geburtshilfe Und Frauenheilkunde

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Adjuvants to increase immunogenicity of SARS-CoV-2 RBD and support maternal–fetal transference of antibodies in mice

Lima

Portilho

Gaspari

2022

Pathogens and Disease

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Adjuvants are important components of vaccines, increasing immunogenicity and modulating the immune response. SARS-CoV-2 vaccines are still being developed in order to improve worldwide access to immunization. Specific populations should be addressed in these investigations, such as pregnant women – to protect both mothers and neonates. In this study, female adult mice were immunized with Receptor-binding domain (RBD) from SARS-CoV-2 adjuvanted by a mixture of DDA and Saponin and put to mating to verify the maternal transference of IgG. For comparison, other group received RBD adjuvanted by OMVs from Neisseria meningitidis and Alum. The adjuvants enhanced IgG production and neutralization. DDA/Sap contributed to increase IgG1, IgG2a, IgG2b and IgG3 isotypes. Total IgG avidity was considered high, as well as IgG1, IgG2a and IgG2b avidity. IgG antibodies were effectively transferred to the offspring, predominantly IgG2a, IgG2b and IgG3. The passive transferred immunoglobulin maintained the neutralizing ability, although it lost avidity. ELISA data was confirmed in Dot-ELISA and Immunoblotting assays. DDA and Saponin seem a promising adjuvant mixture to enhance the humoral response of SARS-CoV-2 antigens. Further studies considering the effects of maternal immunization in the protection of offspring are needed, regardless the platform used in COVID-19 vaccines.

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Advances in the delivery of COVID-19 vaccines

Ita¹

2022

AJBPS

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Several systems are being developed and investigated for the delivery of COVID-19 vaccines. A crucial challenge is the ability to maintain vaccine efficacy through the use of an effective delivery system. Some of these vaccine delivery systems such as lipid nanoparticles (LNPs) have been approved for the use by regulatory authorities in numerous countries. LNPs are currently used for the delivery of Moderna and Pfizer/BioNtech vaccines. LNPs consist of four constituents: Cholesterol for LNP stabilization, cationic lipids for the protection of messenger RNA (mRNA) molecules from nuclease degradation, and helper phospholipids that aid the formation and intracellular release of mRNA and PEGylated lipids that reduce nonspecific interactions. Researchers have also used virus-like particles (VLPs) for COVID-19 vaccine delivery. VLPs consist of several hollow viral proteins without the viral genome. VLPs are structurally identical to the native virus and can activate the human adaptive immune response. The nanosized VLPs self-assemblies have investigated as potential platforms for the delivery of COVID-19 vaccines. Liposomal vesicles are amphiphilic since the polar headgroups of phospholipids are oriented toward water molecules and the hydrophobic chains are in the internal area of the vesicles. The rationale behind the utilization of liposomes as vaccine delivery systems is their versatility and flexibility. Messenger RNA coding for SARS-CoV-2 spike protein can be entrapped into liposomes that are designed to remain stable in the bloodstream until their uptake by phagocytic cells. Other vaccine delivery approaches such as the use of microneedles and electroporation provide transdermal vaccine transport enable COVID-19 vaccines to cross the skin but not the cells of deep-lying tissues.

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Lack of effects on female fertility or pre- and postnatal development of offspring in rats after exposure to AS03-adjuvanted recombinant plant-derived virus-like particle vaccine candidate for COVID-19

Cited by 3 publications

References 46 publications

SARS-CoV-2-Infektion, Corona-Impfung und Reproduktion

SARS-CoV-2-Infektion, Corona-Impfung und Reproduktion

Adjuvants to increase immunogenicity of SARS-CoV-2 RBD and support maternal–fetal transference of antibodies in mice

Advances in the delivery of COVID-19 vaccines

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