“…In the present study, we did not analyze the microbiota composition of the fecal samples as we hypothesized that differences in microbiota composition might explain differences in metabolite production but would not directly explain complaints. In addition, previous studies have already indicated that the microbiota composition did not differ between tolerant and intolerant subjects with lactose malabsorption [ 7 ] nor between subjects with normal lactose digestion and subjects with lactose malabsorption [ 30 ]. Nevertheless, it cannot be excluded that the bacterial techniques used in these studies (fluorescent in situ hybridization (FISH) and bacterial counting after plating, respectively) were not sensitive enough to detect subtle differences in microbiota composition and that current state-of-the-art techniques like next generation sequencing might be more informative.…”
Whether or not abdominal symptoms occur in subjects with small intestinal lactose malabsorption might depend on differences in colonic fermentation. To evaluate this hypothesis, we collected fecal samples from subjects with lactose malabsorption with abdominal complaints (LM-IT, n = 11) and without abdominal complaints (LM-T, n = 8) and subjects with normal lactose digestion (NLD, n = 15). Lactose malabsorption was diagnosed using a 13C-lactose breath test. Colonic fermentation was characterized in fecal samples at baseline and after incubation with lactose for 3 h, 6 h and 24 h through a metabolomics approach using gas chromatography-mass spectrometry (GC-MS). Fecal water cytotoxicity was analyzed using a colorimetric assay. Fecal water cytotoxicity was not different between the three groups (Kruskall-Wallis p = 0.164). Cluster analysis of the metabolite patterns revealed separate clusters for NLD, LM-T and LM-IT samples at baseline and after 24 h incubation with lactose. Levels of 5-methyl-2-furancarboxaldehyde were significantly higher in LM-IT and LM-T compared to NLD whereas those of an unidentified aldehyde were significantly higher in LM-IT compared to LM-T and NLD. Incubation with lactose increased short chain fatty acid (SCFA) concentrations more in LM-IT and LM-T compared to NLD. In conclusion, fermentation patterns were clearly different in NLD, LM-IT and LM-T, but not related to differences in fecal water cytotoxicity.
“…In the present study, we did not analyze the microbiota composition of the fecal samples as we hypothesized that differences in microbiota composition might explain differences in metabolite production but would not directly explain complaints. In addition, previous studies have already indicated that the microbiota composition did not differ between tolerant and intolerant subjects with lactose malabsorption [ 7 ] nor between subjects with normal lactose digestion and subjects with lactose malabsorption [ 30 ]. Nevertheless, it cannot be excluded that the bacterial techniques used in these studies (fluorescent in situ hybridization (FISH) and bacterial counting after plating, respectively) were not sensitive enough to detect subtle differences in microbiota composition and that current state-of-the-art techniques like next generation sequencing might be more informative.…”
Whether or not abdominal symptoms occur in subjects with small intestinal lactose malabsorption might depend on differences in colonic fermentation. To evaluate this hypothesis, we collected fecal samples from subjects with lactose malabsorption with abdominal complaints (LM-IT, n = 11) and without abdominal complaints (LM-T, n = 8) and subjects with normal lactose digestion (NLD, n = 15). Lactose malabsorption was diagnosed using a 13C-lactose breath test. Colonic fermentation was characterized in fecal samples at baseline and after incubation with lactose for 3 h, 6 h and 24 h through a metabolomics approach using gas chromatography-mass spectrometry (GC-MS). Fecal water cytotoxicity was analyzed using a colorimetric assay. Fecal water cytotoxicity was not different between the three groups (Kruskall-Wallis p = 0.164). Cluster analysis of the metabolite patterns revealed separate clusters for NLD, LM-T and LM-IT samples at baseline and after 24 h incubation with lactose. Levels of 5-methyl-2-furancarboxaldehyde were significantly higher in LM-IT and LM-T compared to NLD whereas those of an unidentified aldehyde were significantly higher in LM-IT compared to LM-T and NLD. Incubation with lactose increased short chain fatty acid (SCFA) concentrations more in LM-IT and LM-T compared to NLD. In conclusion, fermentation patterns were clearly different in NLD, LM-IT and LM-T, but not related to differences in fecal water cytotoxicity.
“…From these, we identified 74 further restricted publications, which appeared more relevant but 48 were excluded because they did not compare genetics with the targeted indirect tests, leaving 26. Among these 21 dealt with LBHT (three examined both LBHT and LTT), three dealt with LTT exclusively, and two with potential polymorphisms other than the C/T or G/A types 12, 16, 17, 30–52 …”
Section: Resultsmentioning
confidence: 99%
“…Two of these 10 specifically targeted patients with diagnosis of functional bowel disorders 36, 43 and one selected men at random for comparisons 30 . In the case of the other nine studies, all were volunteers and most were recruited: one study selected random volunteers from hospital out‐patient clinics, 34 seven studies included symptomatic volunteers, one specifically with irritable bowel syndrome 35 and two included volunteers with only self‐reported lactose intolerance 12, 41 . All except the study of Nagy et al.…”
Section: Resultsmentioning
confidence: 99%
“…30 In the case of the other nine studies, all were volunteers and most were recruited: one study selected random volunteers from hospital outpatient clinics, 34 seven studies included symptomatic volunteers, one specifically with irritable bowel syndrome 35 and two included volunteers with only self-reported lactose intolerance. 12,41 All except the study of Nagy et al excluded secondary causes of lactose maldigestion. In this study, 12% (n = 22) of the total included had other diseases.…”
Section: Description Of Included Studiesmentioning
SUMMARY BackgroundThe diagnostic accuracy of two indirect tests of lactose digestion, lactose breath hydrogen and lactose tolerance tests, have not been systematically reviewed for comparison with available publications on genotype.
“…This is one of the largest studies conducted to assess concordance between indirect methods for evaluating lactose malabsorption (HBT and LTT) [7,15,17]. Concordance between HBT and LTT was moderate, which may be due to the fact that HBT depends on colonic flora [18], whereas LTT is influenced by physiologic response to glucose. However, concordance between the two tests improved when the LTT cut-off was set at 15 mg/dL in LTT.…”
ObjectivesLactose malabsorption is generally assessed by hydrogen breath testing (HBT). However, this test is not recommended in patients with high baseline hydrogen concentrations (H2B). In addition, breath testing is not recommended in the current situation created by the COVID-19 pandemic, due to the potential infectiveness of the samples. The objective is to assess concordance between HBT and lactose tolerance test (LTT) depending on H2B concentrations.MethodsA total of 430 patients (40 years, Q1–Q3 = 28–54 years; 66.7% women) suspected of lactose malabsorption were included in the study. Breath and heparinized blood samples were collected at baseline and sequentially after the intake of 50 g of lactose, to measure hydrogen in breath and glycemia in blood, respectively.ResultsH2B was <10 ppm in 69.5% of subjects; 10–20 ppm in 14.7%; and >20 ppm in 15.8% of subjects. In patients with H2B <20 ppm, concordance between HBT and LTT was moderate and consistently improved when the cut-off in LTT was set at 15 mg/dL. The increase in hydrogen and glucose correlated negatively (r=−0.389; p<0.05). The increase in glycemia during LTT was not influenced by H2B levels obtained in HBT.ConclusionsLTT emerges as an alternative to HBT to assess lactose malabsorption in the presence of high H2B levels or when breath testing is not recommended by the circumstances. The best concordance was obtained when the cut-off for LTT was set at 15 mg/dL.
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