Lack of chemopreventive effects of ginger on colon carcinogenesis induced by 1,2-dimethylhydrazine in rats

Dias, Marianne Camargos; Spinardi-Barbisan, Ana Lúcia Tozzi; Rodrigues, M.A.M.; Camargo, João Lauro Viana de; Terán, E.; Barbisan, Luı́s Fernando

doi:10.1016/j.fct.2005.11.015

Cited by 44 publications

(36 citation statements)

References 31 publications

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“…Animals receiving only CO (0.25 mL/100 g body weight) were used as controls (CO group). On the 3rd and 4th weeks of treatment, the CO, βI, GO and βI+GO groups received ip injections of DMH (40 mg/kg body weight) twice a week (27). After 9 weeks of treatment, the animals were euthanized by exsanguination under anesthesia (Figure 2).…”

Section: Animals and Experimental Protocolmentioning

confidence: 99%

“…Colonic segments were stained for 10 min with 0.02% methylene blue in phosphate-buffered saline (PBS; 2.7 mM KCl, 8.1 mM Na 2 PO 4 , 1.5 mM KH 2 PO 4 , 0.14 M NaCl, pH 7.6) (27). Methylene blue-stained ACF, analyzed from the mucosal surface by transmitted light at 40X magnification, appeared as collections of elevated crypts with increased staining and expanded pericryptal spaces (26)(27)(28). The ACF location (distal or proximal colon) and the multiplicity of each focus (number of crypts/ACF) were recorded.…”

Section: Quantification Of Acf In the Colonic Mucosamentioning

confidence: 99%

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Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis

Vieira¹,

Heidor²,

Cardozo³

et al. 2011

Braz J Med Biol Res

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Section: Animals and Experimental Protocolmentioning

confidence: 99%

Section: Quantification Of Acf In the Colonic Mucosamentioning

confidence: 99%

Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis

Vieira¹,

Heidor²,

Cardozo³

et al. 2011

Braz J Med Biol Res

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“…Mammary lesions were classified as alveolar/ductal hyperplasia, colon lesions as ACF, and urothelial lesions as simple or nodular hyperplasia, according to previously published criteria. 26,[28][29][30] Under hematoxylin and eosin staining, apoptotic cells were identified by typical morphological criteria as previously described. 26,30,31 Immunohistochemical staining for proliferation cellular nuclear antigen (PCNA) was performed using the streptavidin-biotin peroxidase method, according to previously established procedures.…”

Section: Processing and Analysis Of Tissuesmentioning

confidence: 99%

“…Colons were stained with 2% methylene blue in phosphate-buffered saline for 10 minutes for ACF counting. 27,28 After ACF analysis, proximal, middle, and distal colon samples were cut into serial strips and processed for paraffin embedding.…”

Section: Processing and Analysis Of Tissuesmentioning

confidence: 99%

Modifying Effects of Lemongrass Essential Oil on Specific Tissue Response to the Carcinogen N-Methyl-N-Nitrosurea in Female BALB/c Mice

Bidinotto

Costa²,

Costa³

et al. 2012

Journal of Medicinal Food

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Lemongrass (Cymbopogon citratus Stapf) essential oil has been used worldwide because of its ethnobotanical and medicinal usefulness. Regarding its medicinal usefulness, the present study evaluated the beneficial effects of lemongrass essential oil (LGEO) oral treatment on cell proliferation and apoptosis events and on early development of hyperplastic lesions in the mammary gland, colon, and urinary bladder induced by N-methyl-N-nitrosourea (MNU) in female BALB/c mice. The animals were allocated into three groups: G1, treated with LGEO vehicle for 5 weeks (five times per week); G2, treated with LGEO vehicle as for G1 and MNU (two injections each of 30 mg/kg of body weight at weeks 3 and 5); and G3, treated with LGEO (five times each with 500 mg/kg of body weight per week) and MNU as for G2. Twenty-four hours after the last MNU application, all animals were euthanized, and mammary glands, colon, and urinary bladder were collected for histological and immunohistochemical analysis.LGEO oral treatment significantly changed the indexes of apoptosis and/or cellular proliferation for the tissues analyzed. In particular, the treatment reduced the incidence of hyperplastic lesions and increased apoptosis in mammary epithelial cells. This increment in the apoptosis response may be related to a favorable balance in Bcl-2/Bax immunoreactivity in mammary epithelial cells. These findings indicate that LGEO presented a protective role against early MNU-induced mammary gland alterations in BALB/c mice.

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“…This inhibiting effect was observed with regard to the entire intestine; however, when only the large intestine was considered, a protective effect was not observed for the development of colon tumors. Dias and colleagues (18) treated rats with 1,2-dimethylhydrazine (DMH) twice a week for 2 weeks after which they fed the rats a ginger extract at 0.5% and 1% of the diet for 10 weeks. Treatment with ginger in the postinitiation phase failed to suppress the formation of aberrant crypt foci (ACF) or the number of crypts per ACF in the DMH-treated group.…”

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confidence: 99%

Ginger: Is it Ready for Prime Time?

Stoner

2013

Cancer Prevention Research

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On the basis of substantial preclinical data showing the preventive efficacy of ginger and its constituents in vitro and in animal models, as well as a phase I pilot trial indicating that ginger extract is well tolerated in humans, Citronberg and colleagues conducted a pilot trial of ginger extract (2 g/day for 28 days) on biomarkers of cell proliferation [human telomerase reverse transcriptase (hTERT), MIB-1], differentiation (p21waf1/cip1), and apoptosis (Bax, Bcl-2) in colonic mucosa from individuals at high-risk for colorectal cancer. Results from the trial suggest that ginger may reduce proliferation in normal-appearing colorectal epithelium and increase apoptosis relative to proliferation, especially in the differentiation zone of colon crypts. The authors suggest that these results support a larger study to confirm the pilot data. Before proceeding with a larger trial, however, it seems prudent to confirm ginger as a chemopreventive for colorectal cancer in animals, particularly when tested in postinitiation protocols and to identify reliable molecular biomarkers of effect that could be evaluated in clinical trials. Pharmacokinetic studies to examine the distribution and localization of ginger compounds and metabolites in the differentiation and proliferative zones of colonic crypts in animals and humans would also be informative. Finally, because the effects of ginger on normal colonic mucosa seem minimal, consideration should be given to the conduct of future trials in humans with premalignant colorectal disease. Cancer Prev Res; 6(4); 257-62. Ó2013 AACR.

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Lack of chemopreventive effects of ginger on colon carcinogenesis induced by 1,2-dimethylhydrazine in rats

Cited by 44 publications

References 31 publications

Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis

Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis

Modifying Effects of Lemongrass Essential Oil on Specific Tissue Response to the Carcinogen N-Methyl-N-Nitrosurea in Female BALB/c Mice

Ginger: Is it Ready for Prime Time?

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