Lack of association between the apolipoprotein B gene 3‘ hypervariable region alleles and coronary artery disease in Finnish patients with angiographically documented coronary artery disease

Abstract

Previous studies have suggested that some apolipoprotein B (apoB) 3' variable number of tandem repeats (3'VNTR) locus alleles are associated with coronary artery disease (CAD). We examined the possible association between the apoB 3'VNTR alleles and CAD in 387 Finnish subjects. Using the polymerase chain reaction and polyacrylamide gel electrophoresis, the 3'VNTR genotype was determined in 187 individuals with severe CAD confirmed by coronary angiography (patients), in 121 individuals with normal coronary angiograms (controls), and in 79 apparently healthy subjects (normals). In contrast to previous reports from other populations, the larger apoB 3'VNTR alleles were not significantly more frequent among CAD patients than among controls or normals. In addition, there was no significant association between the 3'VNTR alleles and serum lipid levels in this Finnish population.

Effect of the 3'APOB-VNTR polymorphism on the lipid profiles in the Guangxi Hei Yi Zhuang and Han populations

“…In a previous study, however, Hegele et al [45] found a significant correlation between Apo B gene polymorphisms and CHD, without any significant association with either LDL or VLDL. There was also no significant association between the 3'VNTR alleles and serum lipid levels in the Finnish population [31]. Similar results were reported in a porcine model of atherosclerosis in which an Apo B genetic variant was associated with atherosclerosis, despite normal lipid levels [46].…”

“…Numerous polymorphisms of the Apo B gene have been described. Particularly, a variable number of tandem repeats (VNTR) polymorphism, which is located 75 bp downstream the second polyadenylation signal at the 3' end of the Apo B gene (2p24-p23) has been found to be common in some ethnic groups [18-21], and it has been reported to be associated with modifications of lipid concentrations [22-28] and the risk of CHD [25-30] in some studies but not in others [31,32]. …”

Apolipoprotein B gene 3′VNTR polymorphism: association with plasma lipids and coronary heart disease in Han Chinese

“…As a highly polymorphic genetic marker, the APOB gene 39VNTR is a powerful tool for different studies, including genome mapping, linkage analysis, forensic and paternity identification, anthropological research, phylogenetic and epidemiological studies (4)(5)(6)(7)(13)(14)(15)(16)(17)(18). Two basic types of 15-nucleotide-long core repeats (X and Y) have been identified.…”

“…The APOB 39VNTR polymorphism can be analyzed by polymerase chain reaction (PCR) amplification and high-resolution agarose (8) or polyacrylamide gel or capillary electrophoresis (9)(10)(11). This polymorphism was reported to be associated with altered lipid levels (12,13) and/or risk of atherosclerosis and coronary heart disease (CHD) (13,14), while other similar studies showed conflicting results (15)(16)(17), although the underlying mechanism remains unknown.…”

Insights from human congenital disorders of intestinal lipid metabolism

“…They were associated with modifi cations of lipid concentrations (267)(268)(269)(270)(271) and the risk of coronary heart diseases ( 270-272 ). Nevertheless, not one of these associations has been consistently observed in a large number of studies ( 273,274 ). Discrepancies may be related to differences of ethnic groups and environmental factors.…”