Lacisediminimonas profundi gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from freshwater sediment

Wu, Xuewen; Jin, Chun-Zhi; Jin, Feng-Jie; Li, Taihua; Sung, Yun Ju; Oh, Hee‐Mock; Lee, Hyung-Gwan; Jin, Long

doi:10.1007/s10482-019-01334-z

Cited by 13 publications

(1 citation statement)

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“…The PCR amplification conditions were set up as follows: 95 °C for 5 min, 30 cycles of 95 °C for 1.5 min, 55 °C for 1 min and 72 °C for 1 min and final extension for 10 min at 72 °C [15]. To get the full 16S rRNA gene, sequencing was carried out with following primers: 27F (5′-AGAGTTTGATCCTGGCTCAG-3′), 785F (5′-GGATTAGATACCCTGGTA-3′), 800R (5′-TACCAGGGTATCTAATCC-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′) [14, 16]. The phylogenetic neighbours of strain BN140002 T were identified using the EzTaxon server [17].…”

Section: Methodsmentioning

confidence: 99%

Salinarimonas soli sp. nov., isolated from soil

Jin

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A light pink coloured bacterium, designated strain BN140002T, was isolated from a soil sample collected in Goesan-gun, Chungcheongbuk-do, Republic of Korea. Cells of strain BN140002T were Gram-stain-negative, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed 94.7, 94.7, 93.9, 93.3, 93.4 and 93.0% similarities to Salinarimonas rosea KCTC 22346T, Salinarimonas ramus DSM 22962T, Saliniramus fredricksonii HL-109T, Microvirga soli R491T, Chelatococcus caeni EBR-4-1T and Chelatococcus composti PC-2T, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and summed feature 1 (C12 : 0 aldehyde and/or unknown 10.98) and the predominant ubiquinone was Q-10. The genomic DNA G+C content of strain BN140002T was 70.1 mol%. The genomic orthoANI values between strain BN140002T and Salinarimonas rosea KCTC 22346T and Salinarimonas ramus DSM 22962T were 75.0 and 74.8 %, respectively. Strain BN140002T had a class I-C type CRISPR-Cas system (CRISPR-associated helicase Cas3, CRISPR-associated protein Cas8c, CRISPR-associated protein Cas7, CRISPR-associated RecB family exonuclease Cas4, CRISPR-associated protein 1, 2). Based on phenotypic, chemotaxonomic and phylogenetic data, strain BN140002T should be assigned as a novel species of the genus Salinarimonas , for which the name Salinarimonas soli sp. nov. is proposed. The type strain is BN140002T (=KCTC 42643T=CCTCC AB 2017173T).

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Section: Methodsmentioning

confidence: 99%