Lacinutrix chionocetis sp. nov., isolated from gut of a red snow crab

Abstract

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated MAB-07, was isolated from the gut of a red snow crab. The novel strain grew optimally at 20 °C, pH 7.0-8.0, and in the presence of 3% (w/v) NaCl. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain MAB-07 belongs to the type strains of species of the genus Lacinutrix. Strain MAB-07 exhibited 16S rRNA gene sequence similarity values of 95.5-97.8% with the type strains of species of the genus Lacinutrix. The predominant cellular fatty acids of strain MAB-07 were iso-C G (27.5%) and iso-C (21.7%). The major respiratory quinine was identified as MK-6. The polar lipids consisted of phosphatidylethanolamine, four unidentified aminolipids, and two unidentified lipids. The genomic DNA G + C content was determined to be 33.3%, and its DNA-DNA relatedness values with the type strains of L. venerupis, L. mariniflava, L. jangbogonensis, L. algicola, and Olleya aquimaris were 28-32%. Based on the data from this polyphasic taxonomic study, strain MAB-07 is considered to represent a novel species of the genus Lacinutrix, for which the name L. chionocetis sp. nov. is proposed. The type strain is MAB-07 (=KCTC 42767 = JCM 30988).

Characterization of Lacinutrix neustonica sp. nov., Isolated from the Sea Surface Microlayer of Brackish Lake Shihwa, South Korea

“…MK-6 was identified as the only menaquinone present in strain HL-RS19 T , which is the same as that found in other Lacinutrix spp. [16,[19][20][21][22][24][25][26][27][28]. The major fatty acids (>5%) of strain HL-RS19 T were iso-C15:1 G (16.5%), iso-C16:0 3-OH (12.9%), anteiso-C15:1 A (9.9%), anteiso-C15:0 (9.7%), iso-C15:0 (9.0%), and iso-C15:0 3-OH (8.3%); a detailed fatty acid composition is given in Table S2.…”

“…MK-6 was identified as the only menaquinone present in strain HL-RS19 T , which is the same as that found in other Lacinutrix spp. [16,[19][20][21][22][24][25][26][27][28].…”

“…Members of the genus Lacinutrix are known to be neutrophilic, Gram-negative, non-motile or motile by gliding, strictly aerobic, straight, slightly rod-shaped, or coccoid cells that are catalase-and oxidase-positive and that produce golden-yellow, orange, or orangeyellow pigments [18][19][20]. The type strains of the genus Lacinutrix have been isolated from diverse marine habitats, such as seawater, sediment, macroalgae, copepod, clam, crab, and flounder, from temperate to polar environments [16,[19][20][21][22][23][24][25][26][27][28]. During a year-round investigation of the SML at a temperate coast near an industrial complex, we isolated a neustonic bacterium identified as belonging to the genus Lacinutrix and designated as HL-RS19 T .…”

Two Novel Lyso-Ornithine Lipids Isolated from an Arctic Marine Lacinutrix sp. Bacterium

“…In addition to L. copepodicola, the polar species includes L. mariniflava, L. algicola [9] and L. jangbogonensis isolated from the Antarctic [10], and L. himadriensis isolated from the Arctic [11]. Species isolated from warm waters include L. iliipiscaria and L. gracilariae isolated from China [12][13][14], L. cladophorae and L. chionocetis from Japan [13,15], L. venerupis from Spain [16] and L. undariae and L. salivirga isolated from South Korea [17,18]. To date, the studies of Lacinutrix sp.…”