‘Lachnoclostridium urinimassiliense’ sp. nov. and ‘Lachnoclostridium phocaeense’ sp. nov., two new bacterial species isolated from human urine after kidney transplantation

Brahimi, S.; Cadoret, F.; Fournier, Pierre‐Edouard; Moal, Valérie; Raoult, Didier

doi:10.1016/j.nmni.2017.01.008

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…Of the 29 species that were not previously detected in humans, 11 were previously recognized taxa. Among the main discoveries, the present study was able to isolate 18 different new species and genera ( Cresci et al, 2016 ; Morand et al, 2016a , b , c ; Brahimi et al, 2017a , b , c , d ; Niang et al, 2019a , b ; Yimagou et al, 2019 ) ( Table 2 ). Of these, seven (39%) were detected in several projects.…”

Section: Resultsmentioning

confidence: 76%

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

et al. 2020

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Human urine was considered sterile for a long time. However, 416 species have been previously cultured, including only 40 anaerobic species. Here, we used culturomics, particularly those targeting anaerobes, to better understand the urinary microbiota. By testing 435 urine samples, we isolated 450 different bacterial species, including 256 never described in urine of which 18 were new species. Among the bacterial species identified, 161 were anaerobes (35%). This study increased the known urine repertoire by 39%. Among the 672 bacterial species isolated now at least once from urine microbiota, 431 (64.1%) were previously isolated from gut microbiota, while only 213 (31.7%) were previously isolated from vagina. These results suggest that many members of the microbiota in the urinary tract are in fact derived from the gut, and a paradigm shift is thus needed in our understanding.

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Section: Resultsmentioning

confidence: 76%

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

et al. 2020

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“…The highest accordance in dDDH value was for “ Lachnoclostridium phocaeense” Marseille-P3177 with 84.6% and a G+C difference of 0.3%. Although this bacterium was described in 2017 ( 105 ), the name “ Lachnoclostridium phocaeense” is still not valid. Taken together, and also considering the topology of both the 16S rRNA gene-based and phylogenomic trees ( Fig.…”

Section: Methodsmentioning

confidence: 99%

Early-Life Immune System Maturation in Chickens Using a Synthetic Community of Cultured Gut Bacteria

et al. 2021

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The gut microbiome is crucial for both maturation of the immune system and colonization resistance against enteric pathogens. Although chicken are important domesticated animals, the impact of their gut microbiome on the immune system is understudied. Therefore, we investigated the effect of microbiome-based interventions on host mucosal immune responses. Increased levels of IgA and IgY were observed in chickens exposed to maternal feces after hatching compared with strict hygienic conditions. This was accompanied by increased gut bacterial diversity as assessed by 16S rRNA gene amplicon sequencing. Cultivation work allowed the establishment of a collection of 43 bacterial species spanning 4 phyla and 19 families, including the first cultured members of 3 novel genera and 4 novel species that were taxonomically described. This resource is available at www.dsmz.de/chibac. A synthetic community consisting of nine phylogenetically diverse and dominant species from this collection was designed and found to be moderately efficient in boosting immunoglobulin levels when provided to chickens early in life. IMPORTANCE The immune system plays a crucial role in sustaining animal health. Its development is markedly influenced by early microbial colonization of the gastrointestinal tract. As chicken are fully dependent on environmental microbes after hatching, extensive hygienic measures in production facilities are detrimental to the microbiota, resulting in low colonization resistance against pathogens. To combat enteric infections, antibiotics are frequently used, which aggravates the issue by altering gut microbiota colonization. Intervention strategies based on cultured gut bacteria are proposed to influence immune responses in chicken.

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“…Lachnoclostridium and Lachnospiraceae_UCG.006 belong to the family Lachnospiraceae in the phylum Firmicutes . Therefore, they had a high degree of similarity (Brahimi, Cadoret, Fournier, Moal, & Raoult, ). Simultaneously, Aciditerrimonas and Acaryochloris overlapped with Gaiella and agricultural_soil_bacterium_SC.I.84 , respectively, suggesting important relationships within those two pairs.…”

Section: Resultsmentioning

confidence: 99%

Water dynamics and microbial communities of bigeye tuna (Thunnus obesus) during simulated cold chain logistics

Wang

Xie

2020

Journal of Food Safety

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This study investigated changes in the physicochemical indexes, water dynamics, and microbial communities of bigeye tuna (Thunnus obesus) during simulated cold chain logistics. With increasing storage time, the color and water activity decreased gradually, while drip loss gradually increased, and pH increased with a fluctuating pattern. The trend of bound water content was not obvious, trapped water declined, and free water increased with fluctuations over time, according to low‐field nuclear magnetic resonance (LF‐1H NMR). High‐throughput sequencing results indicated that fluctuating temperatures inhibited Acinetobacter and Pseudomonas and promoted Pelomonas, Psychrobacter, and Shewanella. Principal component analysis and redundancy analysis revealed significantly different microbial community interactions among the four groups, and environmental factors had distinct effects on bacterial community assemblages. LF‐NMR and high‐throughput sequencing can be used to monitor dynamic quality changes in bigeye tuna during simulated cold chain logistics with fluctuating temperatures.

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‘Lachnoclostridium urinimassiliense’ sp. nov. and ‘Lachnoclostridium phocaeense’ sp. nov., two new bacterial species isolated from human urine after kidney transplantation

Abstract: We describe here the main features of ‘Lachnoclostridium urinimassiliense’ strain Marseille-P2804T (= CSUR P2804) and ‘Lachnoclostridium phocaeense’ strain Marseille-P3177T (= CSUR P3177) that were isolated from urine samples after kidney transplantation in two women.

Cited by 8 publications

References 8 publications

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut

Early-Life Immune System Maturation in Chickens Using a Synthetic Community of Cultured Gut Bacteria

Water dynamics and microbial communities of bigeye tuna (Thunnus obesus) during simulated cold chain logistics

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