Laccases: a never-ending story

Giardina, Paola; Faraco, Vincenza; Pezzella, Cinzia; Piscitelli, Alessandra; Vanhulle, Sophie; Sannia, Giovanni

doi:10.1007/s00018-009-0169-1

Cited by 831 publications

(667 citation statements)

References 173 publications

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“…Laccases have been identified to have wide industrial applications such as in lignin degradation, pulp bleaching, decolourization of different azo recalcitrant textile dyes, wastewater treatment, fossil fuel desulfurization, biosolubilization of coal, degradation of herbicides, bioremediation, detoxification of pollutants, synthesis of natural products like pigments and antioxidants, biosensors and immunochemical applications (Arora and Sharma 2010;Kalyani et al 2012;Lee et al 2012;Rico et al 2014). Laccase enzymes are extensively dispersed in environment among the plants, insects, fungus and bacteria (Giardina et al 2010). White rot fungi is commonly recommended for effective degradation of lignin and laccase production.…”

Section: Introductionmentioning

confidence: 99%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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Purpose Current research focuses on the biological delignification of biomass by microbial laccase which is an environmentally friendly process. Methods Various statistical approaches were designed for optimization of laccase production like Plackett-Burman design as well as response surface methodology (RSM). A laccase mediator system was designed for the delignification of saw dust which was molecularly characterized by high-performance liquid chromatography (HPLC), Fourier transformation infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Results The present study reveals wheat bran is a potential substrate for the production of laccase (63 U/g and 9.6 mg/ g protein) under solid-state fermentation by Trichoderma harzianum strain HZN10. Statistical optimization by RSM using central composite design (CCD) revealed that wheat bran contributed maximally to the overall laccase production followed by yeast extract. Laccase production under optimized conditions yielded 510 U/g with 8.09-fold increase. HPLC peaks representing 4-hydroxy-3-methoxybenzoic (vanillic) acid and 4-hydroxy-3,5-dimethoxybenzoic (syringic) acid showed drastic reduction in laccasetreated saw dust sample indicating the elimination of toxic inhibitors, thereby signifying the detoxification of sample.The laccase-treated saw dust showed 1.6-fold increase in reducing sugars after enzymatic (cellulase) hydrolysis. The FTIR analysis revealed the structural alterations occurring during the delignification process. SEM of biologically treated saw dust revealed the morphological alterations during the delignification process targeting the fiber cell walls rich in lignin. Conclusion The delignification of saw dust was effective by laccase mediator system and was evidenced by HPLC, FTIR and SEM analysis. Hence, laccase can be a powerful tool in biomass to biofuel conversions.

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Section: Introductionmentioning

confidence: 99%

Optimization of laccase production and its application in delignification of biomass

Bagewadi

2017

Int J Recycl Org Waste Agricult

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“…Laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) are blue multicopper oxidases which catalyze the oxidation of an array of aromatic substrates with the reduction of molecular oxygen to water (Giardina et al, 2010). Laccases are known for their importance in the development of efficient biotechnology processes (degradation of different recalcitrant compounds, delignification of lingocellulosics, paper bleaching, bioremediation, dye decolorization and sewage treatment, etc) (Rodriguez et al, 2005;Osma et al, 2010;Shervedani and Amini, 2012).…”

Section: Introductionmentioning

confidence: 99%

Induction and enhancement of the novel marine-derived Alternaria tenuissima KM651985 laccase enzyme using response surface methodology: Application to Azo and Triphenylmethane dyes decolorization

Aty¹,

Hamed²,

El‐Beih³

et al. 2016

J App Pharm Sci

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In our search for a laccase producer from unconventional sources, the marine derived fungus Alternaria tenuissima KM651985 was isolated from decayed wood. It was identified on the basis of morphological and molecular taxonomy and got the Genbank accession number KM651985. Two statistical experimental designs were employed to enhance laccase production. At first, a two level Plackett-Burman design was employed to screen the medium constituents and inducers that significantly affect the enzyme production. Second experiment was important for optimization of the most effective constituents and inducers using central composite design. Applying the above methods revealed that guaiacol (2 mM), copper sulphate (3 mM) and wheat bran (46.82 g/l) were the most effective factors affecting laccase production. A maximal enzyme activity of 91.84 U/ml was more than 6.33-folds the activity obtained using the basal medium. Application of A. tenuissima KM651985 culture medium containing laccase to decolorize two structurally different synthetic dyes was done successfully. This is the first report on the statistical optimization of the marine-derived A. tenuissima KM651985 laccase enzyme and its applications for dyes decolorization.

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“…These enzymes are involved in diverse biological processes, e.g. spore morphology, the lignification of plant cell walls, heavy metal homeostasis, and lignin degradation (Giardina et al, 2010). Laccases can catalyze the oxidation of a wide variety of substrates by use of molecular oxygen as the final electron acceptor and producing water.…”

Section: Introductionmentioning

confidence: 99%