Induction and enhancement of the novel marine-derived Alternaria tenuissima KM651985 laccase enzyme using response surface methodology: Application to Azo and Triphenylmethane dyes decolorization

Abstract

In our search for a laccase producer from unconventional sources, the marine derived fungus Alternaria tenuissima KM651985 was isolated from decayed wood. It was identified on the basis of morphological and molecular taxonomy and got the Genbank accession number KM651985. Two statistical experimental designs were employed to enhance laccase production. At first, a two level Plackett-Burman design was employed to screen the medium constituents and inducers that significantly affect the enzyme production. Second experiment was important for optimization of the most effective constituents and inducers using central composite design. Applying the above methods revealed that guaiacol (2 mM), copper sulphate (3 mM) and wheat bran (46.82 g/l) were the most effective factors affecting laccase production. A maximal enzyme activity of 91.84 U/ml was more than 6.33-folds the activity obtained using the basal medium. Application of A. tenuissima KM651985 culture medium containing laccase to decolorize two structurally different synthetic dyes was done successfully. This is the first report on the statistical optimization of the marine-derived A. tenuissima KM651985 laccase enzyme and its applications for dyes decolorization.

Potential biodegradation of polycyclic aromatic hydrocarbons (PAHs) and petroleum hydrocarbons by indigenous fungi recovered from crude oil-contaminated soil in Iran

“…To the best of our knowledge, this is the first documentation of A. tenuissima 's ability to efficiently biodegrade petroleum-derived PAHs, both in vitro and in soil. A previous study by Abd et al 49 introduced A. tenuissima KM651985, isolated from marine decayed wood, as a novel source of laccase production. This strain exhibits tolerance to saline and alkaline conditions and has demonstrated efficient decolorization of two structurally diverse synthetic dyes, namely congo red and crystal viole 49 .…”

“…A previous study by Abd et al 49 introduced A. tenuissima KM651985, isolated from marine decayed wood, as a novel source of laccase production. This strain exhibits tolerance to saline and alkaline conditions and has demonstrated efficient decolorization of two structurally diverse synthetic dyes, namely congo red and crystal viole 49 . It is worth noting that Laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) are blue multicopper oxidases catalyzing the oxidation of an array of aromatic substrates and are applied in various biotechnological processes such as the degradation of different recalcitrant compounds, bioremediation, dye decolorization, delignification of lignocellulosic, paper bleaching, and sewage treatment 50 , 51 .…”

Valorization of agro-industrial wastes using fungi for industrial enzymes production

“…and brown-rot fungi (Fomitopsis, Laetiporus, Gloeophyllum, etc.) to exudate adequate quantities of lignin-degrading enzymes including lignin peroxidase and laccase that showed high activity in degrading lignin-matter of woody substrates into phenolic compounds; also Abd El Aty et al [52] enhancement the production of laccase enzyme from the marine-derived Alternaria tenuissima KM651985. Extracellular production of amylase enzyme from flamentous fungi like marine Alternaria alternata hydrolyzes starch (the plants storage material) into glucose [53,54].…”

“…Horticultural wastes [63] Pleurotus ostreatus Peanut shell [64] Alternaria alternate Wheat bran [32] Alternaria tenuissima Wheat bran [52] α-Amylase Aspergillus oryzae Coconut oil cake, [65] Aspergillus niger black gram bran, and soybean [66] Aspergillus niger Papaya waste [67] Alternaria alternate corn cobs, wheat bran, potato shells, wheat straw and rice straw [53] Xylanase…”

“…In addition, filamentous fungi are usually preferred for SSF processes, due to their specific ability to colonize the inter-particular spaces of solid waste matrices, and to produce various enzymes that, hydrolyze the solid substrate into fermentable sugars, and valuable products [82]. Aspergillus, Alternaria, Trichoderma and Pleurotus, were the most genera employed in this technique [52,83].…”