Laccase–MWCNT–chitosan biosensor—A new tool for total polyphenolic content evaluation from in vitro cultivated plants

Diaconu, Mirela; Liţescu, Simona Carmen; Radu, Gabriel Lucian

doi:10.1016/j.snb.2010.01.064

Cited by 125 publications

(65 citation statements)

References 46 publications

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“…Different types of voltammetry on stationary electrodes (Ziyatdinova and Budnikov 2005) including chemically modified (Xiao et al 2006;Guo et al 2009) have been developed for polyphenols detection. Amperometric laccase-based biosensors for the determination of polyphenols in wines (Gomes and Rebelo 2003;Fernandes and Rebelo 2009) and plant extracts are described (Diaconu et al 2010). Biosensor containing enzyme tyrosinase immobilized by the crosslinking method has been used for detection of polyphenols in tea (Abhijith et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Novel Coulometric Approach to Evaluation of Total Free Polyphenols in Tea and Coffee Beverages in Presence of Milk Proteins

2010

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Electrogenerated hexacyanoferrate(III) ions have been used as coulometric titrant for the determination of total free polyphenols in beverages. Stoichiometric coefficients of natural flavonoids (rutin, quercetin, and taxifolin) in their reaction with [Fe(CN) 6 ] 3− ions were established. The number of electrons involved in the oxidation corresponds to number of phenolic OH groups in the molecule of polyphenols. Proteins (casein and bovine serum albumin) bind polyphenols in the complexes that leads to significant decrease of free polyphenols portion (in the range of 5-76%). Ferric reducing power (FRP) of eight tea and 17 coffee samples was determined. The absolute FRP values ranged from 224 to 405 and from 241 to 488 Ccup −1 for tea and coffee, respectively. Effect of milk proteins on total free polyphenols of tea and coffee and appropriate alteration of beverages FRP were evaluated. Milk addition decreases FRP of drinks in the range of 10-70% reflecting high content of inactive polyphenols.

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Section: Introductionmentioning

confidence: 99%

Novel Coulometric Approach to Evaluation of Total Free Polyphenols in Tea and Coffee Beverages in Presence of Milk Proteins

2010

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“…studied the properties of glassy polymeric electrodes modified with poly (caffeic acid) films towards the CA determination17. In addition, the lead modified GCE (PbFE-GCE), laccase-MWCNT and gold nanoparticles modified electrodes were reported for the electrochemical determination of CA181920. Recently, the chemically reduced and electrochemically reduced graphene oxide modified electrodes are investigated for the determination of CA which revealed a superior performance2122.…”

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confidence: 99%

A voltammetric determination of caffeic acid in red wines based on the nitrogen doped carbon modified glassy carbon electrode

Karikalan

Chen

2017

Sci Rep

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We reported an electrochemical determination of caffeic acid (CA) based on the nitrogen doped carbon (NDC). The described sensor material was prepared by the flame synthesis method, which gave an excellent platform for the synthesis of carbon nanomaterials with the hetero atom dopant. The synthesized material was confirmed by various physical characterizations and it was further characterized by different electrochemical experiments. The NDC modified glassy carbon electrode (NDC/GCE) shows the superior electrocatalytic performance towards the determination of CA with the wide linear concentration range from 0.01 to 350 μM. It achieves the lowest detection limit of 0.0024 μM and the limit of quantification of 0.004 μM. The NDC/GCE-CA sensor reveals the good selectivity, stability, sensitivity and reproducibility which endorsed that the NDC is promising electrode for the determination of CA. In addition, NDC modified electrode is applied to the determination of CA in red wines and acquired good results.

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“…The steady-state current response of the CA is reached within 3 s, which is an indicating that the fast electrochemical oxidation of CA at 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 the modified electrode surface. However, the detection limit (LOD) of the CA at the 3DG/MWCNTsÀNc modified electrode substantially lower than that of the other modified electrodes such us, Nafion/GRNC modified electrode (LOD = 91 nM) [16], MWCNT/VTMS fabricated electrode (LOD = 0.22 mM) [38], Pencil graphite electrode (LOD = 88.3 nM) [21], AuNP/GN sensor electrode (LOD 50.0 nM) [19], Gold/Chitosan nanocomposite modified electrode (LOD = 25.0 nM) [39], C-SPE/RGO/Nafion decorated modified electrode (LOD = 0.22 mM) [40], Tyrosinase/diazonium/SPGE modified biosensor electrode (LOD = 0.2 mM) [41] and Lacc-CS-MWCNT modified CA sensor electrode (LOD = 151 nM) [42]. Figure 6B shows the linear relation between the oxidation peak current and the CA concentration.…”

Section: Electrochemical Determination Of Camentioning

confidence: 99%

Hydrothermal Synthesis of Three Dimensional Graphene‐Multiwalled Carbon Nanotube Nanocomposite for Enhanced Electro Catalytic Oxidation of Caffeic Acid

2016

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Three dimensional graphene‐multiwalled carbon nanotube nano composite (3DG/MWCNTs−Nc) was synthesized by simple hydrothermal method for the amperometric determination of caffeic acid (CA). The prepared nanocomposite was characterized by scanning electron microscopic technique (SEM), ultraviolet‐visible spectroscopy (UV), Raman spectroscopy and infrared spectroscopy (IR). Moreover, the interfacial electron transfer properties of the modified electrode were carried out by the electro chemical impedance spectroscopy (EIS). Besides, the electro chemical performance of the modified electrode was carried out by the cyclic voltammetry (CV) and amperometric (i‐t) technique. The proposed electrode was exhibited an enhanced electrocatalytic activity towards the detection of CA. Under the optimal condition, the 3DG/MWCNTs−Nc modified electrode displayed a linear range from 0.2 to 174 μM, detection limit (LOD) 17.8 nM and sensitivity of 5.8308 μA μM−1 cm−2 and on applied potential + 0.2 V. These result showed, 3DG/MWCNTs−Nc modified electrodes showed good repeatability, reproducibility, and higher stability. In addition, the fabricated electrode was then successfully used to determine the CA in real samples with satisfactory recoveries. Which suggests that the 3DG/MWCNTs−Nc as a robust sensing materials for the electrochemical detection of CA.

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Laccase–MWCNT–chitosan biosensor—A new tool for total polyphenolic content evaluation from in vitro cultivated plants

Cited by 125 publications

References 46 publications

Novel Coulometric Approach to Evaluation of Total Free Polyphenols in Tea and Coffee Beverages in Presence of Milk Proteins

Novel Coulometric Approach to Evaluation of Total Free Polyphenols in Tea and Coffee Beverages in Presence of Milk Proteins

A voltammetric determination of caffeic acid in red wines based on the nitrogen doped carbon modified glassy carbon electrode

Hydrothermal Synthesis of Three Dimensional Graphene‐Multiwalled Carbon Nanotube Nanocomposite for Enhanced Electro Catalytic Oxidation of Caffeic Acid

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