Laccase-mediator system for enzymatic degradation of carbonaceous matter in the sequential pretreatment of double refractory gold ore from Syama mine, Mali

Sakai, Ryotaro; Mendoza, Diego M.; Konadu, Kojo T.; Cindy, None; Aoki, Yuji; Hirajima, Tsuyoshi; Ichinose, Hirofumi; Sasaki, Keiko

doi:10.1016/j.hydromet.2022.105894

Cited by 7 publications

(2 citation statements)

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“…Normally, the KM should not vary much if it was a pure enzyme, but because it is an enzymatic complex, there was a synergism between the intra and extracellular enzymes present. This synergism allowed the potentiation of the complex (Mattila et al, 2022;Bernal et al, 2021;Sakai et al, 2022), obtaining an almost complete degradation of the ICD in the concentration of 50 ppm in 25 days of experimentation, above 90% in Gi2 and above 80% in Gi1 (Table 2).…”

Section: Enzymatic Kinetics Of Extractsmentioning

confidence: 95%

“…The lignin degradation occurs because of multi-enzymatic process resulting from the coordinated action of a series of intra and extracellular enzymes such as oxidoreductases, as well as other oxidases producing hydrogen peroxide and by intermediate metabolites of low molecular mass (Mattila et al, 2022;Bernal et al, 2021;Sakai et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Bioremediation potential of industrial laundry effluent by agaricomycetes from brazilian tropical dry forest

Barrera

Neto

Oliveira

et al. 2022

RSD

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Mycotic bioremediation of effluents from industrial jeans laundries is a necessary biotechnological treatment to prevent contamination of water bodies. In phase I, the discoloration of Indigo Carmine Dye (ICD) and Textile Effluent (TE) by seven species of Agaricomycetes from the brazilian tropical dry forest (Caatinga) was evaluated. First, nutritional stress was caused by Nitrogen Limitation (NL) at three experimental times, T1 (1 day). T2 (4 days) and T3 (7 days). In phase II, microorganisms were cultivated in the initial growth times Gi1 (10 days) and Gi2 (25 days), Without Addition of Nutrients (WAN) and stress was induced by NL (T1). Subsequently, ICD and TE discoloration tests continued for 28 days. In the ecotoxic analysis, the biotreated samples in phase II were tested on nauplii of Artemia HIGH 5 without the addition of food. In phase I, the percentages of ICD and TE discoloration were greater than 55% using fungi F1, F2, F5 and F6 for 10 days without sterility. In phase II, the best percentages of discoloration were found for TE in Gi1 and for ICD in Gi2, with F1 and F5 (identified by molecular biology). The results showed that Gi1 (WAN) increased the biodegradation of TE and Gi2 (WAN) favored the biodegradation of ICD, in T1 (NL) without sterility. The best enzymatic activity of laccase and lignin peroxidase was presented in F5. The enzyme extracts had a Michaelis-Menten kinetic behavior. All samples of TE bioremediated in phase II no showed toxicity on Artemia sp. in 48 hours of experimentation.

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Section: Enzymatic Kinetics Of Extractsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%