Laccase-mediated functionalization of natamycin by gallic acids for the therapeutic effect on Aspergillus fumigatus keratitis

Ji, Xiaoyue; Peng, Xudong; Long, Xinggui; Zhang, Yingxue; Lin, Jing; Yin, Jiao; Zhang, Ranran; Zhao, Guiqiu

doi:10.1016/j.ejphar.2022.175041

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…The LMW compounds, including phenolic compounds, indoles and other secondary metabolites, exhibit antioxidative, antibacterial, anticancer and other activities [6][7][8], while the HMW compounds primarily consist of peptides and enzymes [6]. Among these enzymes, laccase stands out as a significant HMW fraction with considerable potential for pharmaceutical and industrial applications [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microb Cell Fact

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Background White-rot fungi are known to naturally produce high quantities of laccase, which exhibit commendable stability and catalytic efficiency. However, their laccase production does not meet the demands for industrial-scale applications. To address this limitation, it is crucial to optimize the conditions for laccase production. However, the regulatory mechanisms underlying different conditions remain unclear. This knowledge gap hinders the cost-effective application of laccases. Results In this study, we utilized transcriptomic and metabolomic data to investigate a promising laccase producer, Cerrena unicolor 87613, cultivated with fructose as the carbon source. Our comprehensive analysis of differentially expressed genes (DEGs) and differentially abundant metabolites (DAMs) aimed to identify changes in cellular processes that could affect laccase production. As a result, we discovered a complex metabolic network primarily involving carbon metabolism and amino acid metabolism, which exhibited contrasting changes between transcription and metabolic patterns. Within this network, we identified five biomarkers, including succinate, serine, methionine, glutamate and reduced glutathione, that played crucial roles in co-determining laccase production levels. Conclusions Our study proposed a complex metabolic network and identified key biomarkers that determine the production level of laccase in the commercially promising Cerrena unicolor 87613. These findings not only shed light on the regulatory mechanisms of carbon sources in laccase production, but also provide a theoretical foundation for enhancing laccase production through strategic reprogramming of metabolic pathways, especially related to the citrate cycle and specific amino acid metabolism.

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Section: Introductionmentioning

confidence: 99%

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Zhang,

Qiu,

Qiu

et al. 2024

Microb Cell Fact

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“…It is generally used in the dairy industry to preserve cheeses as it presents excellent activity against surface-contaminating fungi and does not modify the sensorial characteristics of the product [43]. Several studies report the effectiveness of Natamycin in inhibiting the growth of Penicillium sp [45,46], Aspergillus sp [47,48], and Cladosporium sp [45,49], with most fungi and yeasts being inhibited by 0.5-6 μg /ml and 1.0 -5.0 μg /ml of this compound, respectively [50].…”

Section: Natamycinmentioning

confidence: 99%

“…However, selecting an appropriate encapsulation system must consider the interactions between the wall material with the antimicrobial compounds and the components of the food matrix to ensure better chemical stability of the substance and its delivery to the target [116]. More research is needed since most studies on dairy matrices are focused on milk [117,118], yogurts [102,115], and coating films for cheeses [48,119]. Dairy products are commonly exposed to microorganisms that can spoil the food and cause harm to human health.…”

Section: Encapsulation Of Compounds With Antimicrobial Activity In Da...mentioning

confidence: 99%

Natural Antimicrobials in Dairy Products: Benefits, Challenges, and Future Trends

Soutelino,

Silva,

Rocha

2024

Preprint

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This review delves into using natural antimicrobials in the dairy industry and examines various sources of these compounds, including microbial, plant, and animal sources. It highlights dairy products' most commonly used natural antimicrobials, such as nisin, lysozyme, lactoferrin, chitosan, and essential oils. It discusses the mechanisms by which they inhibit microbial growth and improve the shelf life of dairy products. Moreover, the review explores encapsulation technology as a promising approach for increasing the viability of natural antimicrobials. It discusses how encapsulation technology can protect these compounds from environmental factors that can reduce their effectiveness, such as pH, temperature, and oxygen exposure. The review also highlights the different materials that can be used for encapsulation, such as alginate, chitosan, and starch, and discusses their properties and suitability for encapsulating different natural antimicrobials. Finally, the review examines the benefits and challenges of using natural antimicrobials in dairy products. While natural antimicrobials offer several advantages, including improved safety, quality, and sensory properties of dairy products, it's crucial to be aware of the challenges associated with their use, such as potential allergenicity, regulatory requirements, and consumer perception. The review concludes by emphasizing the need for further research to identify and develop effective and safe natural antimicrobials for the dairy industry to ensure the quality and safety of dairy products for consumers.

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Therapeutic proteins: developments, progress, challenges, and future perspectives

Kumar,

Barwal,

Sharma

et al. 2024

3 Biotech

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Laccase-mediated functionalization of natamycin by gallic acids for the therapeutic effect on Aspergillus fumigatus keratitis

Cited by 8 publications

References 48 publications

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613

Natural Antimicrobials in Dairy Products: Benefits, Challenges, and Future Trends

Therapeutic proteins: developments, progress, challenges, and future perspectives

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