Laccase immobilization and surface modification of activated carbon fibers by bio-inspired poly-dopamine

Zhang, Chencheng; Gong, Lili; Mao, Qinghui; Han, Ping; Lü, Xiaoping; Qu, Jiangang

doi:10.1039/c8ra01265b

Cited by 38 publications

(20 citation statements)

References 53 publications

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“…Immobilization process may block laccase active sites and thereby decrease enzyme substrate affinity and maximize reaction rate (Antecka et al 2018). Low V max for laccase immobilized on both surface modified and unmodified activated carbon fibers were reported by (Zhang et al 2018).…”

Section: Kinetic Constants Of Free and Immobilized Enzymementioning

confidence: 99%

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Paul²,

Sunkar

2021

Water Science and Technology

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Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzyme immobilized onto Zein – Polyvinyl pyrrolidone composite nanofiber is done. The nanofibers were characterized by SEM and FTIR analysis. pH and temperature profiles of immobilized enzyme were found to be broader than its free counterpart. The Km value was found to be 0.243 mM for free laccase and 0.958 mM for immobilized laccase. Similarly Vmax for the free enzyme was 3.572 U/mg compared to 2.48 U/mg of immobilized laccase. The relative activity of immobilized laccase was 64.91% after storage for 30 days at room temperature while it was 28.64% for free laccase. The temperature and pH for AR 1 decolorization were optimized and was found to be 60 °C and 5 respectively. Also decolorization percentage was found to be 91.67% for immobilized laccase and 72.03% of free laccase in the presence of natural mediators like vanillin. From phytotoxicity studies it was found that the germination rate, shoot and root length was increased compared to untreated dye. Therefore, Zein-PVP nanofiber immobilized laccase could be an ideal candidate for the textile dye decolorization.

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Section: Kinetic Constants Of Free and Immobilized Enzymementioning

confidence: 99%

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Paul²,

Sunkar

2021

Water Science and Technology

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“…This occurred because most of the AOX was adsorbed on the surface of the activated carbon. Thus, the active sites on the surface were occupied and further adsorption was inhibited [36]. We therefore concluded that the optimal activated carbon dosage to achieve maximum AOX removal efficiency was 1.75 g l −1 .…”

Section: Resultsmentioning

confidence: 99%

“…This occurred because most of the AOX was adsorbed on the surface of the activated carbon. Thus, the active sites on the surface were occupied and further adsorption was inhibited [36]. We therefore concluded that the optimal adsorption capacity (mg g -1 ) 0 where q e is the adsorption capacity (mg g 21 ) and C e is the AOX concentration after adsorption (mg l 21 ).…”

Section: Effect Of the Activated Carbon Dosage On The Adsorption Capacitymentioning

confidence: 94%

Adsorptive removal of adsorbable organic halogens by activated carbon

et al. 2018

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Current research mainly focuses on the reduction of adsorbable organic halogen (AOX) sources, while studies on AOX monitoring and management in the environment are scarce. Organic pollutants in water are mainly fixed by sediments. Thus, in this paper, activated carbon was used to simulate the adsorption of AOX by sediments. AOX volatilization and degradation were also studied to exclude their effect on adsorption. Micromolecule chlorides were more easily volatilized and degraded than chlorobenzene and chlorophenol. The adsorption of activated carbon to AOX in bleaching wastewater was also studied and the optimum conditions for AOX removal were elucidated (particle size, 62 µm; time, 120 min; pH, 2.5; temperature, 40°C; and activated carbon dosage, 1.75 g l−1). AOX adsorption by activated carbon is a chemical process. Hence, the chemical compositions of the bleaching effluent with and without adsorption were analysed by GC-MS. The results revealed that activated carbon exhibits a good AOX removal effect, thereby providing a theoretical basis for monitoring the AOX distribution in the environment.

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“…Enzyme immobilization is defined as the attachment of soluble enzymes to a support material that results in the reduction or total loss in mobility of the attached enzyme [13]. Immobilizing laccase on various supports can protect them from denaturation, improve their stability, maintain good catalytic efficiency, facilitate efficient recovery and lead to more economical process [10] [14]. Thus, it is important to define an appropriate and suitable support for laccase immobilization [10].…”

Section: Methods Of Laccase Immobilizationmentioning

confidence: 99%

A Short Review on Recent Development of Laccase Immobilization on Different Support Materials

Jafri¹,

Rahman²,

Ali³

2021

J-SuNR

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Laccase is a bio catalytic agent and multi-copper enzyme containing oxidases that are potentially great for oxidizing large number of phenolic and non-phenolic compounds. However, drawbacks do arise when laccase use in large scale; low in stability, high production cost, non-reusability, sensitive towards denaturing and poor storage ability of free enzymes. These problems lead to the progress in laccase immobilization in order to facilitate the efficient recovery and re-use of the enzyme, thus enabling cost-effective in continuous processes. Apart from discussing on different methods in laccase immobilization such as entrapment, encapsulation and cross-linking in general, we have reviewed a recent development in laccase immobilization on different supports or carriers binding (natural and synthetic). Future works are recommended to focus on innovative strategies on the modified supports to improve the enzyme immobilization as well as sensible entrapment techniques for industrial applications.

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Laccase immobilization and surface modification of activated carbon fibers by bio-inspired poly-dopamine

Abstract: In this study, we developed a new synthesis method for modifying activated carbon fibers (ACFs) by dopamine with oxidation-based self-polymerization (DA-ACFs).

Cited by 38 publications

References 53 publications

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Adsorptive removal of adsorbable organic halogens by activated carbon

A Short Review on Recent Development of Laccase Immobilization on Different Support Materials

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