Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

G, Ranimol; Paul, Chinju; Sunkar, Swetha

doi:10.2166/wst.2021.200

Cited by 10 publications

(5 citation statements)

References 38 publications

(37 reference statements)

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“…Small peaks at 2872, 647, and 576 cm -1 which were absent in PLA; however, available in PVP pointed out that there was an association between PLA and PVP. These peaks are also in accordance with the studies of Ranimol et al (2021) who reported similar results for neat PVP. A slight shift was noted in the peak from 1269 cm -1 to 1291 cm -1 upon essential oil incorporation to the PLA-PVP fiber.…”

Section: Ir-spectra Of the Fibrous Webssupporting

confidence: 93%

Fabrication of Mandarin (Citrus reticulate L.) peel essential oil and nano-calcium carbonate incorporated polylactic acid/polyvinylpyrrolidone electrospun webs

Parın

Sıcak

Eliuz

et al. 2022

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Fibrous materials from polylactic acid (PLA) and polyvinylpyrrolidone (PVP) containing mandarin peel essential oil (MPEO) and/or nano-calcium carbonate were prepared by electrospinning process. The electrospun webs were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and microbiological tests. IR spectra results indicated the existence of interaction among additives and polymer matrix. Obtained SEM results showed that incorporation of essential oil to the polymer matrix have a remarkable effect on the fiber morphology. Essential oil incorporation increased the fiber diameters. The electrospun composite webs were ineffective against Acinetobacter baumannii, Bacillus subtilis, Escherichia coli, Enterococcus fecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus.

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Section: Ir-spectra Of the Fibrous Webssupporting

confidence: 93%

Fabrication of Mandarin (Citrus reticulate L.) peel essential oil and nano-calcium carbonate incorporated polylactic acid/polyvinylpyrrolidone electrospun webs

Parın

Sıcak

Eliuz

et al. 2022

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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“…Therefore, storage stability becomes a crucial factor to be credited when designing a robust biocatalyst 47 . Thus, it is expected that the heterogeneous biocatalysts maintain their catalytic activity even over the days of storage 84 . In this study, the immobilized and free laccase stability was measured from storage at 5°C for 30 days.…”

Section: Resultsmentioning

confidence: 99%

“…47 Thus, it is expected that the heterogeneous biocatalysts maintain their catalytic activity even over the days of storage. 84 In this study, the immobilized and free laccase stability was measured from storage at 5 C for 30 days. Figure 8 shows the performance of the tested two enzymes.…”

Section: Storage Stabilitymentioning

confidence: 99%

Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

Vieira

Henriques

Gurgel

et al. 2023

J of Applied Polymer Sci

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This work evaluates the potential of polyacrylonitrile particles (PAN) as support for laccase from Trametes versicolor immobilization. The slurry polymerization method forms mesoporous particles with low surface area and low maximum pore volume in desorption. The particles were chemically modified by consecutive alkaline and acid hydrolysis, followed by amination and activation with glutaraldehyde to enzyme immobilization. The laccase immobilization yield was 99.48% and 14.29% using the functionalized and non-functionalized particles, respectively. The enzyme activity was measured by the oxidation of 2,2 0 -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) at different pHs and temperatures. The PAN/laccase derivative was hyperactivated at pH 3, up to 3 times higher than the free enzyme, and performed better at 50 C after 6 h of incubation, with relative activity up to 33% higher than the free enzyme. However, both enzymes denatured when the conditions reached pH 8 and 70 C. The PAN/laccase retained 89% of the initial activity after 30 days of storage at 5 C. It was possible to reuse the enzymatic derivative for 5 cycles, with up to 50% residual activity, under 50 C and pH 3. These results show the potential of this new support for laccase immobilization and further applications of industrial interest.

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“…However, the decolorization was affected when temperature and pH were increased up to a certain level. It led to enzyme inactivation due to high temperature and the dispersion of dye molecules across the matrix due to the increase in pH (Ranimol et al 2021). The decolorization increases with the time duration and equilibrium was sorted out and the temperatures above 40 o C inhibit the enzyme activity leading to diminishing the decolorization (Iqbal et al 2021).…”

Section: Dye Decolorization Box-behnken Methodsmentioning

confidence: 99%

Ameliorating Direct Blue Dye Degradation Using Trametes versicolor Derived Laccase Enzyme Optimized through Box–Behnken Design (BBD) via Submerged Fermentation

et al. 2022

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The major intend of this study was to elucidate the laccase production by Trametes versicolor under submerged fermentation using fruit waste peel as substrate. The textile dye was decolorized by the procured crude enzymatic extract using the response surface methodology. The submerged media with organic fruit peel waste extract (jackfruit, pineapple & kaffir) supplemented with gypsum, calcium carbonate, and nutrient broth were considered superior for laccase production. The produced laccase enzyme was used in dye decolorization at the optimum conditions using the Box-Behnken design. Subsequently, the experiment was designed with four variables (dye concentration, pH, temperature & time) with three factors to achieve the maximum direct blue dye decolorization. The highest laccase activity level was obtained from jackfruit peel extract with 3.86U/ml on 15th day at 25oC with pH 5.0 when compared to the other two extracts. The maximum laccase activity with guaiacol was obtained at optimum pH 4 and 40oC. The predicted value was experimentally validated by attaining 81.25% of dye color removal. From the result, the optimum conditions for direct blue color removal were: dye concentration 40ppm, pH 4.0, temperature 40oC at 24 hours. From the results of this study, it was concluded that the jack fruit peel was a more suitable substrate for laccase production. The dye decolorization results were recommended that Box-Behnken design for parameters optimization. The T. versicolor laccase was more proficient for textile dye decolorization. The opportunity was created by using the laccase enzyme for the biological treatment of textile dyeing effluent before discharging into the environment.

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Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Cited by 10 publications

References 38 publications

Fabrication of Mandarin (Citrus reticulate L.) peel essential oil and nano-calcium carbonate incorporated polylactic acid/polyvinylpyrrolidone electrospun webs

Fabrication of Mandarin (Citrus reticulate L.) peel essential oil and nano-calcium carbonate incorporated polylactic acid/polyvinylpyrrolidone electrospun webs

Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

Ameliorating Direct Blue Dye Degradation Using Trametes versicolor Derived Laccase Enzyme Optimized through Box–Behnken Design (BBD) via Submerged Fermentation

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