Laboratory simulation of microbial enhanced oil recovery using Geobacillus toebii R-32639 isolated from the Handil reservoir

Abstract

Microbial enhanced oil recovery (MEOR) is a biological based technology to enhance crude oil (CO) recovery at old oil wells by manipulating function or structure or both of the microbial environments existing in oil reservoirs. Even if many biosurfactant-producing microbes can be found in different environments, the ability of Geobacillus toebii R-32639 to enhance CO recovery needs to be verified. In this study, artificial cores (ACs) are used to simulate the application of MEOR by three scenarios of injecting nutrients, microbial culture and bioproducts. The use of Geobacillus toebii R-32639 as biosurfactant producer enables a lowering by 25.3% of interfacial tension and by 14.1% of CO viscosity and also enables the degrading of a 7.4-28.8% fraction of (C 12 -C 34 ) hydrocarbons after 7 days of incubation. The injection of microbial culture into a laboratory-scale CO reservoir of the AC for the desirable purpose of enhanced oil recovery could feasibly achieve an average recovery factor of 14.27% but lower by 5.50% the AC porosity and by 91.0% the AC permeability; this is the best-case scenario of the microbial core flooding (MCF) experiments. The simulation of MEOR by using bacterial strains of Geobacillus toebii R-32639 provides valuable insight into the use of MCF experiments to obtain greater oil production from existing reservoirs.

Fundamentals of mass transfer and kinetics for biosorption of oil and grease from agro-food industrial effluent by Serratia marcescens SA30

“…The results ( Fig. 4 and 5 42 to having a great effect on the actual rates of global, external and internal mass transfer when the concentration of O&G in AFIE as the sources of carbon and energy for such biosurfactant-producing bacteria increases. Due to Serratia marcescens SA30 strains accumulated O&G intracellularly (see Fig.…”

Kinetics of substrate utilization and bacterial growth of crude oil degraded by Pseudomonas aeruginosa

“…The creation and evolution of technology for oil and gas industry can increase the capacity of oil and gas production [ 1 , 2 ]. However, the operation of oil and gas refineries may negatively affect the environmental quality, including air and water pollution [ 3 ].…”

Microbial enhanced oil recovery: interfacial tension and biosurfactant-bacteria growth

“…Koya-Goshu model has the most parameters to be estimated compared to all the previous models Table 3 lists the detailed experimental data, usage in this research, bacteria name or type, medium of cultivation, and the supplied nutrient. Detailed composition through GCMS analysis of Handil Field crude oil is available in the literature (Fulazzaky et al 2015). All data have incubation temperature of 50-55 °C.…”