Laboratory rearing of the legume pod borer, Maruca testulalis Geyer (Lepidoptera: Pyralidae) on a semi-synthetic diet

Onyango, Fredrick; Ochieng‘-Odero, J. P. R.

doi:10.1017/s174275840001818x

Cited by 10 publications

(14 citation statements)

References 1 publication

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“…However, in our study, no decline was observed after 8 successive generations of laboratory rearing, and the fecundity of the M. vitrata females was higher than that found in Onyango and Ochieng'-Odero's work (1993), in which the rearing density was 5 larvae per container (7.5 × 2.5 cm). Therefore, we consider that D-New and a medium rearing density (15–25 larvae per box, 10 cm diameter) are suitable for continuous rearing.…”

Section: Discussioncontrasting

confidence: 85%

New Artificial Diet for Continuous Rearing of the Bean Pod Borer,Maruca vitrata

Wang

Zheng

et al. 2013

Journal of Insect Science

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The bean pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae), is a serious pantropical pest of grain legumes. A suitable artificial diet is desirable for producing uniform insects for commercial purposes or research. Three previously described artificial diets, 1 newly-developed artificial diet, and cowpea (Vigna unguiculata (L.) Walp. (Fabales: Fabaceae)), the natural hostplant of M. vitrata, were used for rearing M. vitrata, and the life parameters were examined. The results indicated that insects completed a full life cycle only when the larvae were fed cowpea or the diet reported by Onyango and Ochieng'-Odero (1993), called the “D-OO diet.” However, the rearing efficiency (i.e., larval and pupal survival, longevity of adults, and fecundity) on the D-OO diet was inferior to the rearing efficiency on cowpea. Subsequently, a new artificial diet was formulated based on soybean powder, Glycine max (L.) Merr. (Fabales: Fabaceae), and wheat germ, Triticum aestivum L. (Poales: Poaceae). The egg production, egg hatching, larval developmental duration, and pupal survival of the M. vitrata reared on the new artificial diet were found to be significantly improved relative to the D-OO diet, but were not significantly better than on the host-plant cowpea. The optimum rearing density was 15–25 larvae per box. There were no significant changes in reproductive potential after 8 successive generations of rearing on the new diet. These results indicated that the newly developed diet could serve as a viable alternative to cowpea plant for continuous rearing of M. vitrata.

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Section: Discussioncontrasting

confidence: 85%

New Artificial Diet for Continuous Rearing of the Bean Pod Borer,Maruca vitrata

Wang

Zheng

et al. 2013

Journal of Insect Science

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“…This was followed by 30.6 ± 3.1, 26.6 ± 4.1, 21.8 ± 2.1 and 18.8 ± 1.1 in modified D-OOD, D-OOD, D-NEW and D-OO diet, respectively. These findings are in close agreement with Wang et al (2013) who reported rearing of M. vitrata for 8 successive generations without decline in the performance on new artificial diets and partially line with Onyango and Ochieng-Odero (1993) who reported that Maruca reared on soybean flour and cowpea flower powder as base ingredients in semi-synthetic diet for 10 successive generations.…”

Section: Results and Discussion I Comparison Of Developmental Paramesupporting

confidence: 91%

Refinement and evaluation of artificial diet for rearing of legume pod borer, Maruca vitrata Geyer (Lepidoptera: Crambidae)

Rachappa¹,

Hanchinal²,

Shekhara³

et al. 2017

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The investigation on refinement and evaluation of artificial diet for M. vitrata, were carried out during 2014 and 2015 under walk in plant growth chamber established at ARS, Gulbarga, Karnataka, India. Comparison of developmental parameters of three successive generations of M. vitrata reared on different diets revealed that, maximum survival of egg, larva and pupa was 78.4 ± 3.1, 67.6±5.5 and 73.0±5.0 respectively in modified D-OOD diet and was superior over rest of the diets (D-OOD, D-New and D-OO) while it was on par with natural host plant. Maximum fecundity of 62.34±4.1 eggs/ female was observed in refined D-OOD diet was on par with pigeonpea host plant and superior over other diets. Biology studies of Maruca on refined artificial diet in comparison with natural host plant pigonpea were revealed that, incubation period (2.6 ± 0.46 and 2.85 ± 0.40 days), total larval duration (14.28± 1.02 and 14.78±1.52 days), pupal period (9.41±0.70 days and 10.33±1.08 days) did not vary significantly between modified diet and natural host. Fecundity in refined artificial diet was 66.00 ± 4.00 and it was 65.99± 7.02 eggs on natural host plant. Per cent egg hatching was almost same in both the cases.

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“…The larvae from the field were introduced to a semisynthetic diet prepared following the procedure described by Onyango and Ochieng'-Odero (1993), and the culture was maintained in the rearing room at 26-308C, 60 -75% relative humidity (RH) and a 12 hr of light:12 hr of dark photoperiod. When larvae pupated, the pupae were carefully harvested from the diet using a pair of soft forceps onto Petri plates (14 cm diameter) and later introduced into a sleeved Perspex glass cage (30 £ 30 £ 30 cm).…”

Section: Insectsmentioning

confidence: 99%

Pathogenicity and performance of two candidate isolates of Metarhizium anisopliae and Beauveria bassiana (Hypocreales: Clavicipitaceae) in four liquid culture media for the management of the legume pod borer Maruca vitrata (Lepidoptera: Crambidae)

Tumuhaise

Ekesi

Mohamed

et al. 2015

Int. J. Trop. Insect Sci.

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Pathogenicity and performance of two candidate isolates of Metarhizium anisopliae and Beauveria bassiana (Hypocreales: Clavicipitaceae) in four liquid culture media for the management of the legume pod borer Maruca vitrata (Lepidoptera: Crambidae)Abstract. The aim of this study was to identify and develop entomopathogenic fungi as biopesticides for management of Maruca vitrata (Fabricius). Fourteen isolates of Metarhizium anisopliae (Metchnikoff) Sorokin and six of Beauveria bassiana (Bals.-Criv.) Vuill. were screened against first instar larvae of M. vitrata. The two most virulent isolates were selected and further tested for production in four liquid media. Different formulations of one isolate were evaluated against different developmental stages. Metarhizium anisopliae ICIPE 18 and ICIPE 69 caused highest larval mortality of 91 and 81%, with lethal time to 50% mortality (LT 50 ) values of 1.8 and 1.7 days and LC 50 of 1.07 £ 10 7 and 3.01 £ 10 6 conidia/ml, respectively. ICIPE 69 yielded more biomass and propagules in two liquid media than ICIPE 18. All developmental stages and adult moths were susceptible to fungal infection and horizontal transmission caused a significant reduction in fecundity. Isolate ICIPE 69 was identified as the most potent isolate for managing M. vitrata based on demonstrated efficacy against different developmental stages and amenability to production in liquid media and could be utilized as an alternative to pesticides in cowpea IPM.

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Laboratory rearing of the legume pod borer, Maruca testulalis Geyer (Lepidoptera: Pyralidae) on a semi-synthetic diet

Cited by 10 publications

References 1 publication

New Artificial Diet for Continuous Rearing of the Bean Pod Borer,Maruca vitrata

New Artificial Diet for Continuous Rearing of the Bean Pod Borer,Maruca vitrata

Refinement and evaluation of artificial diet for rearing of legume pod borer, Maruca vitrata Geyer (Lepidoptera: Crambidae)

Pathogenicity and performance of two candidate isolates of Metarhizium anisopliae and Beauveria bassiana (Hypocreales: Clavicipitaceae) in four liquid culture media for the management of the legume pod borer Maruca vitrata (Lepidoptera: Crambidae)

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