Laboratory Guide to Insect Pathogens and Parasites

Poinar, George O.; Thomas, Gerard M.

doi:10.1007/978-1-4684-8544-8

Cited by 65 publications

(52 citation statements)

References 75 publications

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“…Also separating it from the Laboulbeniales is the wasp host since the only known hymenopteran hosts of Laboulbeniales are ants (Hymenoptera: Formicidae) [7] and in general, Hymenoptera are very infrequently parasitized by fungi [4,5]. It is obvious that Spheciophila adercia gen et sp.…”

Section: Discussionmentioning

confidence: 99%

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A Mid-Cretaceous Ectoparasitic Fungus, Spheciophila adercia gen et sp. nov., Attached to a Wasp in Myanmar Amber

Poinar¹

2016

Fungal Genom Biol

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Section: Discussionmentioning

confidence: 99%

“…Today, while several groups of fungi parasitize the integument of arthropods [4,5], the most common and only group that forms thalli on the surface of arthropods is the Laboulbeniales [6,7]. This Order consists of small, microscopic obligate ectoparasites that normally occur on the integument of their hosts.…”

Section: Introductionmentioning

confidence: 99%

A Mid-Cretaceous Ectoparasitic Fungus, Spheciophila adercia gen et sp. nov., Attached to a Wasp in Myanmar Amber

Poinar¹

2016

Fungal Genom Biol

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“…The mosquitoes were identified by morphological characteristics (Nagpal et al 1995). The dead, paralyzed, moribund, irritable/sluggish, fragile, putrefied, blackish and fluid oozing larvae were recorded as the unhealthy insects (Poinar and Thomas 1984). The normal, healthy larvae were surface-sterilized by 70 % ethanol (5 min) and washed three times with sterile distilled water (Chatterjee et al 2010).…”

Section: Collection Of Mosquito Larvaementioning

confidence: 99%

“…The gut extracts were diluted up to 10 -2 level with sterile distilled water and pour plated on 5 plates at 100 ll/100 ml with nutrient agar (g/l: peptone 5, beef extract 3, agar 3, pH 7) media and cultured in B.O.D incubator at 30 ± 0.1°C for 24 h and maintained at 4 ± 0.1°C in a refrigerator on nutrient agar (Poinar and Thomas 1984;Lacey 1997).…”

Section: Isolation Of the Bacterial Isolatesmentioning

confidence: 99%

Characterization and control of symbiotic Bacillus cereus isolated from the mid gut of Anopheles subpictus Grassi

Mukhopadhyay

Chatterjee

2016

J Parasit Dis

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An investigation was done to find out the role of gut bacterium on the larval development and survival of Anopheles subpictus, the vector responsible for the transmission of malaria. An. subpictus mosquitoes breed exclusively in stagnant water, including shrimp/fish ponds with high nutrient level. One bacterial strain (CX2) was isolated from the midgut of late third instar of mosquito larvae. The bacterial isolate was sensitive to recommended doses of tetracyclin (30 lg/disc), doxycycline (30 lg/disc), kanamycin (30 lg/disc), gentamycin (10 lg/disc), streptomycin (10 lg/disc), vancomycin (30 lg/disc), ofloxacin (5 lg/disc), levofloxacin (5 lg/disc), gatifloxacin (10 lg/ disc), but resistant to ampicillin (10 lg/disc).The larvae which were fed with the mixture of two antibiotics tetracylin (30 lg/disc) and doxycyclin (30 lg/disc) (1:1) could not survive in rice-field water. In the control experiments without any antibiotic treatment, 95-100 % survival and 95 % adult emergence were observed. The study indicates that the elimination of gut bacteria suppressed larval growth. Phylogenetic analysis of the 16S rRNA gene sequence was also done. Based on the morphological, biochemical, FAME analysis and phylogenetic analysis, the bacterial isolate CX2 was identified as Bacillus cereus. Poly acrylamide gel electrophoresis analysis revealed that the isolate showed discrete bands ranging from 24.272 to 60.049 kDa proteins. Water extract and methanol extract of Tamarindus indica showed inhibitory effect against B. cereus.

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“…Con un microscopio estereoscopio Leica se separaron por sexo. Posterior-mente, a 20 machos se les realizaron cortes de los genitales, cabeza, palpos, tibias y alas, estas estructuras se prepararon en laminillas con azul de algodón para aclarar los tejidos (Poinar y Thomas, 1984). Para su estudio se usó un microscopio óptico Leica M80 y una cámara modelo DFC295 (Software Leica Application Suite V3.0 ® ) de la misma marca; se tomaron fotografías de las estructuras importantes para la identificación de especies (todas las mediciones en este estudio se realizaron con un microscopio y software).…”

Section: Identificación De Especies Del Mosquito Fungoso Negrounclassified

Biología de Lycoriella ingenua y Bradysia impatiens (Diptera: Sciaridae)

Marín-Cruz

Cibrián-Tovar

Méndez-Montiel

et al. 2015

MYB

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ResumenSe estudiaron dos especies de mosquito fungoso negro, Lycoriella (Lycoriella) ingenua (Dufour, 1839) y Bradysia impatiens (Johannsen, 1912) (Diptera: Sciaridae), que causan daño a plántulas de Pinus montezumae (Lamb, 1832) en viveros forestales. Estos dípteros se identificaron morfológicamente y por métodos moleculares y se citan por primera vez como plaga en viveros forestales de México. La proporción sexual para L. ingenua fue de un macho por cada 1.25 hembras; para caracterizar esta especie se midieron huevos, pupas, larvas y cápsulas cefálicas. Con los datos morfométricos de longitud de cuerpo y ancho de la cápsula cefálica de larvas y mediante el método de conglomerados jerárquicos se determinaron los estadios larvarios de la especie. Para B. impatiens por cada macho existen 2.4 hembras. En la distinción de los estadios larvarios, el mejor parámetro fue el ancho de cápsula cefálica. Ambas especies presentaron cuatro estadios larvarios. Las dos especies se criaron en laboratorio a 21 ºC ± 2 ºC. El ciclo completo de L. ingenua requirió de 30.5 días ± 3.5 días. Para B. impatiens el tiempo para concluir ciclos biológicos fue de 27.5 días ± 2.5 días. Se determinó que las larvas de ambas especies consumen la raíz de Pinus montezumae. Se identificaron los hongos asociados a larvas y adultos de ambas especies, Fusarium circinatum fue el más importante y se registra por primera vez en viveros de P. montezumae; esta especie de hongo se identificó mediante morfología y por métodos moleculares.Palabras clave: ciclo biológico, estadios larvarios, hongos asociados, mosquito fungoso negro, proporción de sexos. AbstRActLycoriella (Lycoriella) ingenua (Dufour, 1839) and Bradysia impatiens (Johannsen, 1912) (Diptera: Sciaridae) are two black fungus gnats species studied that harm Pinus montezumae (Lamb, 1832) seedlings in forestry nurseries. These dipterous were morphological and molecularly identified and are mentioned for the first time as a pest in Mexico's forestry nurseries. The proportion of sexes to Lycoriella ingenua was of one male per 1.25 females; to characterize this species eggs, pupas, larvae and cephalic capsules were measured. The morphometric data of body length and cephalic cage width of larvae through the hierarchical conglomerated method determined the larval instars of the species. For B. impatiens there are 2.4 females per male. In the definition of the larval instars, the best parameter was that of the cephalic capsule. Both species presented four larval instars. Both species were bred under lab conditions at 21 °C ± 2 °C. The whole cycle of L. ingenua required 30.5 ± 3.5 days. For B. impatiens the time to conclude biological cycles was of 27.5 ± 2.5 days. It was determined that both species of larvae consume Pinus montezumae root. The fungi associated to larvae and fungi of both species were identified. Fusarium circinatum was the most important and it is registered for the first time in nurseries of Pinus montezumae; this species was morphological and molecularly identified.

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Laboratory Guide to Insect Pathogens and Parasites

Cited by 65 publications

References 75 publications

A Mid-Cretaceous Ectoparasitic Fungus, Spheciophila adercia gen et sp. nov., Attached to a Wasp in Myanmar Amber

A Mid-Cretaceous Ectoparasitic Fungus, Spheciophila adercia gen et sp. nov., Attached to a Wasp in Myanmar Amber

Characterization and control of symbiotic Bacillus cereus isolated from the mid gut of Anopheles subpictus Grassi

Biología de Lycoriella ingenua y Bradysia impatiens (Diptera: Sciaridae)

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