Laboratory Diagnosis of Cystic Fibrosis

Webster, H. L.; Rundell, Clark

doi:10.3109/10408368209085074

Cited by 44 publications

(19 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rate of evaporation from the open end of the spiral capillary tube of the Macroduct is nil (0·16 ìl h¢) (Webster, 1983). Pilot data showed a 97% recovery when Microduct collectors were spiked with an 18 mmol l¢ ethanol standard (Sigma) for 15 min.…”

Section: Methodsmentioning

confidence: 98%

“…Sweat was harvested from an approximately 7 cmÂ area on the flexor surface of the forearm for 15 min immediately following pilocarpine iontophoresis using a Macroduct sweat collector (Wescor, Logan, UT, USA). The rate of evaporation from the open end of the spiral capillary tube of the Macroduct is nil (0·16 ìl h¢) (Webster, 1983). Pilot data showed a 97% recovery when Microduct collectors were spiked with an 18 mmol l¢ ethanol standard (Sigma) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Sweat ethanol concentrations are highly correlated with co-existing blood values in humans

Buono¹

1999

Exp. Physiol.

View full text Add to dashboard Cite

summaryThis study compared the concentration of ethanol, both absolute and relative to water content, in sweat and blood. Ten male volunteers consumed approximately 13 mmol (kg body weight)¢ of ethanol. Blood and sweat samples were collected approximately 1, 2 and 3 h following ingestion. Sweat was collected following pilocarpine iontophoresis using an anaerobic technique that prevented ethanol evaporation. In addition, the water content of sweat and blood samples was determined. The correlation between sweat and blood ethanol, expressed in mmol l¢, was r = 0·98. The slope of the relationship was 0·81. When corrected for the water content in each sample, and expressed as mmoles per litre of water, the correlation remained very high (r = 0·97) while the slope increased to 1·01. These results suggest that rapid and complete equilibrium of ethanol occurs across the sweat gland epithelium.

show abstract

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Sweat ethanol concentrations are highly correlated with co-existing blood values in humans

Buono¹

1999

Exp. Physiol.

View full text Add to dashboard Cite

show abstract

“…Pilocarpine iontophoresis was standardized by Gibson and Cooke in 1959 (Quantitative Pilocarpine Iontophoretic Test [or QPIT] method [89]). Subsequently, in 1983, a simplified version of sweat collection via pilocarpine iontophoresis was introduced (Wescor Macroduct system [100]). Pilocarpine iontophoresis is the gold-standard sweat-testing method for diagnosing cystic fibrosis (CF) [101]; however, it is important to note that the sweating response differs significantly between pharmacological and thermal and/or exercise-induced sweating.…”

Section: Methodological Sources Of Variability In Sweating Rate and Smentioning

confidence: 99%

Sweating Rate and Sweat Sodium Concentration in Athletes: A Review of Methodology and Intra/Interindividual Variability

2017

View full text Add to dashboard Cite

Athletes lose water and electrolytes as a consequence of thermoregulatory sweating during exercise and it is well known that the rate and composition of sweat loss can vary considerably within and among individuals. Many scientists and practitioners conduct sweat tests to determine sweat water and electrolyte losses of athletes during practice and competition. The information gleaned from sweat testing is often used to guide personalized fluid and electrolyte replacement recommendations for athletes; however, unstandardized methodological practices and challenging field conditions can produce inconsistent/inaccurate results. The primary objective of this paper is to provide a review of the literature regarding the effect of laboratory and field sweat-testing methodological variations on sweating rate (SR) and sweat composition (primarily sodium concentration [Na+]). The simplest and most accurate method to assess whole-body SR is via changes in body mass during exercise; however, potential confounding factors to consider are non-sweat sources of mass change and trapped sweat in clothing. In addition, variability in sweat [Na+] can result from differences in the type of collection system used (whole body or localized), the timing/duration of sweat collection, skin cleaning procedure, sample storage/handling, and analytical technique. Another aim of this paper is to briefly review factors that may impact intra/interindividual variability in SR and sweat [Na+] during exercise, including exercise intensity, environmental conditions, heat acclimation, aerobic capacity, body size/composition, wearing of protective equipment, sex, maturation, aging, diet, and/or hydration status. In summary, sweat testing can be a useful tool to estimate athletes’ SR and sweat Na+ loss to help guide fluid/electrolyte replacement strategies, provided that data are collected, analyzed, and interpreted appropriately.

show abstract

“…Pilocarpine, an acetylcholine agonist, was delivered via reagent-impregnated (0.5% pilocarpine) solid agar gel discs (Pilogel, Wescor). Sweat was collected for 15 min immediately after iontophoresis using a Macroduct sweat collector (Wescor) according to the procedures outlined by Webster (1983). A different forearm site was used on each of the 3 measurement days to avoid any potential effects of desensitization of the glands from repeated pharmacological stimulation (Chen and Elizondo, 1974).…”

Section: Methodsmentioning

confidence: 99%