Laboratory Diagnosis of Amebiasis

Proctor, E. M.

doi:10.1016/s0272-2712(18)30523-7

Cited by 29 publications

(17 citation statements)

References 67 publications

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“…These methods are expensive and time-consuming, making them impractical for epidemiological surveys. The commercial development of antigen detection kits and cDNA probes [27] for dot-blot hybridization may provide more rapid, accurate, and less costly diagnostic procedures for the future [28], but it is necessary to evaluate any advantages in epidemiological surveys.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of antibodies againstEntamoeba histolyticain Mexico measured by ELISA

et al. 1995

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SummaryThe prevalence of antibodies againstEntamoeba histolyticawas studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture ofEntamoeba histolyticaHM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60538 samples suppliedbythe National Seroepidemiology Survey, antibodies were found in 4·49 % (4·32–;4·65% at 95 % confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1·25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country.Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame.

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Section: Discussionmentioning

confidence: 99%

Prevalence of antibodies againstEntamoeba histolyticain Mexico measured by ELISA

et al. 1995

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“…The microscopic examination was accomplished by normal saline preparation and Lugol's Iodine preparation directly from the stool. (7) The negative samples were examined by formol ether concentration technique. (8) Modified Ziehl-Neelsen stain was used for the identification of coccidian parasites only in case of HIV infected patients.…”

Section: Methods Of Stool Examinationmentioning

confidence: 99%

Prevalence of Intestinal Parasitic Infections in Patients Attending a Tertiary Care Hospital in Eastern Bihar

Kumar¹,

Biswas²,

Yasmin³

et al. 2014

jemds

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“…Iodine stains make the nucleus perfectly visible. The appearance of chromatoid bodies is the same as in wet mount preparations (164). Although several other stains, including Giemsa, methylene blue, Chorazole black E, Wright's, and iodine-trichrome, may be used successfully, Wheatley's trichrome staining or one of the modified iron hematoxylin stains for permanent smears has been suggested for routine use in the diagnosis of E. histolytica/E.…”

Section: Microscopymentioning

confidence: 97%

“…In wet mounts, the trophozoite nuclei cannot easily be seen (164 (164). If a fresh stool specimen cannot be examined immediately, it should be preserved with a fixative such as polyvinyl alcohol or kept cool (4°C).…”

Section: Microscopymentioning

confidence: 99%

“…If a fresh stool specimen cannot be examined immediately, it should be preserved with a fixative such as polyvinyl alcohol or kept cool (4°C). Occasionally motile trophozoites are seen even after 4 h at this temperature (170,229), although the trophozoites generally disintegrate rapidly in unfixed stool specimens (164). Stool specimens can be examined either unstained or stained with Lugol's or D'Antoni's iodine.…”

Section: Microscopymentioning

confidence: 99%

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Laboratory Diagnosis of Amebiasis

2003

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The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal E. histolytica. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples

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Laboratory Diagnosis of Amebiasis

Cited by 29 publications

References 67 publications

Prevalence of antibodies againstEntamoeba histolyticain Mexico measured by ELISA

Prevalence of antibodies againstEntamoeba histolyticain Mexico measured by ELISA

Prevalence of Intestinal Parasitic Infections in Patients Attending a Tertiary Care Hospital in Eastern Bihar

Laboratory Diagnosis of Amebiasis

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