Laboratory determinations in Anisakis simplex allergy+

Pozo, M. D. Del; Moneo, Ignacio; Corrés, L. Fernández de; Audícana, M.; Muñoz, D.; Fernández, E.; Navarro, J. A.; García, Milagros

doi:10.1016/s0091-6749(96)80073-x

Cited by 71 publications

(56 citation statements)

References 15 publications

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“…Although the pattern of IgE bands in immunoblot testing of our volunteers differed from that reported by Del Rey, who found a strong presence of anti-Anisakis IgE, our data are similar to those of Del Pozo, who demonstrated the presence of anti-Anisakis IgG and absence of anti-Anisakis IgE antibodies in the sera of their subjects (Del Pozo et al 1996).…”

Section: Discussionsupporting

confidence: 77%

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Figueiredo

Vericimo

Cardoso

et al. 2013

Acta Parasitologica

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Although the incidence of anisakiasis is rising worldwide, its frequency is still unknown in Brazil. The aim of this study was to verify immunoreactivity to Anisakis simplex antigens in healthy adults and determine its possible relationship with frequency of fish consumption and allergy symptoms. A prospective cross-sectional study was carried out with 67 volunteers recruited from a military facility in Niterói, Brazil. The subjects completed a structured questionnaire and serum titers of specific anti-Anisakis IgE and IgG antibodies were measured. The association between frequency of fish intake and IgE reactivity was evaluated by Fisher's exact test. Almost all subjects (97.0%, 65/67) that consumed seafood; 64.6% (42/65) ate fish at least once weekly. Of all seafood consumers, 56.9% (37/65) reported allergy symptoms, being gut allergies most often cited (35.5%). IgE seroreactivity to Anisakis simplex was found in 20.9% of subjects (14/67), with 13.4% (9/67) reacting exclusively to somatic antigen, 3.0% (2/67) exclusively to excretory/secretory antigens and 4.5% (3/67) to both antigens. There was a significant association between frequency of fish consumption and positive serology (p = 0.019). An immunoblot assay for Anisakis antigens showed different positive bands for IgG. The direct relationship between ELISA reactivity and frequency of fish intake and absence of association with allergy symptoms suggests previous contact with Anisakis simplex antigens.

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Section: Discussionsupporting

confidence: 77%

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Figueiredo

Vericimo

Cardoso

et al. 2013

Acta Parasitologica

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“…In these cases, specific IgE determination against the parasite not only cannot be considered to be a reliable indicator of allergy but also can be a confusing factor, since such antibody has been detected in 25% of otherwise healthy controls (27,83). This is not surprising, since cross-reactivity has also been described for inhalant allergens from a wide variety of sources, and 43% of subjects with positive IgE to pneumoallergens do not present respiratory symptoms (248).…”

Section: Sensitization Without Allergic Symptomsmentioning

confidence: 99%

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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SUMMARY Infection of humans with the nematode worm parasite Anisakis simplex was first described in the 1960s in association with the consumption of raw or undercooked fish. During the 1990s it was realized that even the ingestion of dead worms in food fish can cause severe hypersensitivity reactions, that these may be more prevalent than infection itself, and that this outcome could be associated with food preparations previously considered safe. Not only may allergic symptoms arise from infection by the parasites (“gastroallergic anisakiasis”), but true anaphylactic reactions can also occur following exposure to allergens from dead worms by food-borne, airborne, or skin contact routes. This review discusses A. simplex pathogenesis in humans, covering immune hypersensitivity reactions both in the context of a living infection and in terms of exposure to its allergens by other routes. Over the last 20 years, several studies have concentrated on A. simplex antigen characterization and innate as well as adaptive immune response to this parasite. Molecular characterization of Anisakis allergens and isolation of their encoding cDNAs is now an active field of research that should provide improved diagnostic tools in addition to tools with which to enhance our understanding of pathogenesis and controversial aspects of A. simplex allergy. We also discuss the potential relevance of parasite products such as allergens, proteinases, and proteinase inhibitors and the activation of basophils, eosinophils, and mast cells in the induction of A. simplex-related immune hypersensitivity states induced by exposure to the parasite, dead or alive.

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“…El diagnóstico se confirma al observar la larva, cuando es expectorada o extraída mediante endoscopia de zonas accesibles (esófago, estómago, colon); sin embargo, es más difícil cuando la larva migra hacia el intestino delgado o es extra-gastrointestinal, requiriendo con frecuencia cirugía (2,(12)(13)(14)(15)(16)(17), o cuando la infestación se hace cró-nica y la larva es destruida por el sistema inmune (7,(18)(19)(20). El diagnóstico inmunológico se basa en la prueba cutánea con extracto crudo de larva de A. simplex y en la determinación de IgE específica frente a este (21)(22)(23)(24). La utilización de alérgenos purificados de excreción-secreción (Ani s 1, Ani s 4) (25,26), anticuerpos monoclonales (UA3, que reconoce el alérgeno Ani s 7) (27) y la medición de proteína catiónica del eosinófilo (28) han mejorado el diagnóstico de esta parasitosis, principalmente en las formas alérgicas.…”

Section: Introductionunclassified

Utilidad de la anamnesis y de las técnicas inmunológicas en el diagnóstico de la anisakidosis en pacientes con abdomen agudo

Rey-Moreno

Valero-López²,

Gomez-Pozo

et al. 2008

Rev. esp. enferm. dig.

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Utilidad de la anamnesis y de las técnicas inmunológicas en el diagnóstico de la anisakidosis en pacientes con abdomen agudo A. del RESUMENIntroducción: Anisakis simplex puede producir síntomas digestivos y alérgicos. En este trabajo se evalúan los antecedentes epidemiológicos y los resultados inmunológicos para diferenciar entre pacientes con anisakidosis y aquellos con otras patologías digestivas que cursan con dolor abdominal.Pacientes y método: estudio de cohortes realizado con 134 pacientes: 52 fueron diagnosticados de anisakidosis por los hallazgos quirúrgicos y anatomopatológicos y/o seroconversión especí-fica frente a A. simplex (grupo A) y en 82 pacientes la anisakidosis fue excluida como diagnóstico (grupo NA: no-anisakidosis). Se han evaluado el antecedente de ingesta de pescado crudo, la prueba cutánea en prick (PC) y el inmunoblot IgE como elementos de diagnóstico.Resultados: los pacientes de los grupos A y NA mostraron resultados diferentes respecto a la de ingesta de pescado crudo (p < 0,0001) y la PC (p < 0,0001), con valores predictivos negativos (VPN) del 98,39 y 95,56% y positivos (VPP) del 70,83 y 87,50%, respectivamente. En el inmunoblot, se halló una banda de aproximadamente 60 kDa en el 86,2 y 19,2% de los pacientes del grupo A y NA, respectivamente (VPP: 62,50%; VPN: 94,03%).Conclusiones: en pacientes con dolor abdominal, la ingesta de pescado crudo o poco cocinado tiene elevada sensibilidad y VPN (98,39%) pero menor VPP (70,83%), por lo que nos ayuda principalmente a descartar la anisakidosis. La ausencia de sensibilización cutánea al extracto crudo de A. simplex apoya la ausencia de anisakidosis con una probabilidad alta (95,56%). La presencia de una banda de 60 kDa en el inmunoblot podría ser útil para su diagnóstico.Palabras clave: Anisakidosis. Anisakis simplex. Abdomen agudo. Anamnesis. Prueba cutánea. IgE específica. Inmunoblot. ABSTRACTIntroduction: Anisakis simplex can be a cause of digestive symptoms. Our aim was to evaluate the epidemiological antecedents and immunological data available for a differentiation between patients with anisakidosis and those with other acute abdominal problems.Patients and methods: this is a prospective cohort study involving 134 patients with acute abdominal problems: 52 patients were diagnosed with anisakidosis by means of surgical and pathological findings and/or specific IgE seroconversion against Anisakis simplex (group A), and in 82 patients anisakidosis had been ruled out (group NA: non-anisakidosis). We evaluated the antecedent of raw fish ingestion, the skin prick test, and IgE immunoblotting as diagnostic tools.Results: patients in groups A and NA differ in terms of prior raw fish ingestion (p < 0.0001) and positive SPT (p < 0.0001), with their respective negative predictive values (NPV) being 98.39% (95%CI: 90.17-99.92) and 95.56% (95%CI: 83.64-99.23). Regarding immunoblotting, in 86.2% of patients in group A a band of 60 kDa was detected, which was also detected in 19.2% of patients in group NA.Conclusions: a negative answer to the qu...

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Laboratory determinations in Anisakis simplex allergy+

Cited by 71 publications

References 15 publications

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

Utilidad de la anamnesis y de las técnicas inmunológicas en el diagnóstico de la anisakidosis en pacientes con abdomen agudo

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