Laboratory detection and identification ofAspergillusspecies by microscopic observation and culture: the traditional approach

Abstract

Molecular and immunologic tests promise better, faster laboratory diagnosis of aspergillosis, but microscopy and culture remain commonly used and essential tools. Procedural changes, as well as adequate training of laboratory professionals, can enhance the value of these traditional tools. Using Blankophor or Calcofluor for microscopic examinations; improving recognition of morphologic characteristics of opportunistic fungi in stained smears of specimens; maximizing the growth rate and production of conidia by Aspergillus spp. in culture; and recognizing atypical variants of common aspergilli can improve the laboratory's contribution to rapid diagnosis. Surveys indicate that the number of laboratory professionals is declining as the demand for healthcare is rising. Effective recruitment, retention, and training of personnel must be concurrent with advances in technology.

EFFECT OF SOME ECOLOGICAL FACTORS ON THEGROWTH OF <I>ASPERGILLUS NIGER</I> AND <I>CLADOSPORIUM SPHAEROSPERMUM</I>

“…They have been implicated in human health problems, damage to building materials, books, clothes and stored foods (Burshtein et al, 2011;Rahoma, 2011;Chadeganipour et al, 2010;Haleem et al, 2009;Tasic and Miladinovic-Tasic, 2007;McClenny, 2005;Unlu et al, 2003;Yano et al, 2003).…”

Synthesized zinc peroxide nanoparticles (ZnO₂-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

“…The color and mycelial growth were observed daily. In addition, the spores-producing A. niger was identified up to the genus and species level based on macroscopic and microscopic criteria 44 as shown in supplementary additional files ( Figure S1). …”

“…Phenotypic identification and conventional biochemical tests for bacteria and fungi were performed. [42][43][44] Moreover, isolated yeasts were identified according to the protocols. 45,46 In order to confirm the phenotypic observation of P. aeruginosa and A. niger from polymicrobial cultures, P. aeruginosa were re-inoculated on cetrimide agar supplemented with 15 µg/mL nalidixic acid and checked by using VITEK ® 2 automated systems (BioMérieux, Marcy-L'E'toile, France).…”

PRODUCTION & PURIFICATION OF A LOW MOLECULAR WEIGHT EXTRACELLULAR TANNASE FROM ASPERGILLUS TPF-4

“…using manual about the genus Aspergilli [14] [15] and designated as Aspergillus TPF-4( Fig. 1) Fig 1: Growth of AspergillusTPF-4 on potato dextrose agar plate.…”

“…using manual about the genus Aspergilli [14] [15] and designated as Aspergillus TPF-4. Inoculation of Aspergillus TPF-4 spores was done on PDA (Potato Dextrose Agar) plates, grown at 25 o C for 6 days and storage was done at 4°C.…”