Laboratory contaminants in lipid chemistry: Detection by thin‐layer chromatography and infrared spectrophotometry and some procedures minimizing their occurrence

Rouser, George; Kritchevsky, Gene; Whatley, Mary C.; Baxter, Claude F.

doi:10.1007/bf02533000

Cited by 42 publications

(3 citation statements)

References 6 publications

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“…Cineole was estimated by the Ortho-Cresol method 17. Separation of lipid classes of the oil into three major lipid classes, neutral lipid, glycolipid and phospholipid by silicic acid column chromatography on about 150 g. oil followed by Rouser et. al 18 .…”

Section: Methodsmentioning

confidence: 98%

Characterization of the Eucalyptus ( E. Globulus) Leaves Oil

Khan

Khatun

Hossain

et al. 2012

J. Bangla. Chem. Soc.

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Studies were carried out on the characterization of Eucalyptus globulus leaves oil. It is found that Eucalyptus globulus tree bears a lot of leaves which are grown under the soil texture and climatic condition of Bangladesh contain a fair amount of pale yellow coloured volatile oil. The oil of the leaves were extracted by hydro distillation method during the month of July to December. The highest yield (3.5%) of the oil was obtained from the maturity leaves of the tree in the month of July and the lowest yield (0.08) in the month of December. Physico-chemical characteristics of the extracted oil like saponification value, acid value, iodine value, specific gravity, refractive index, optical rotation and separation of lipid classes were studied by the conventional methods and the results suggested that the oil can be used suitably for the preparation of pharmaceutical and industrial products. DOI: http://dx.doi.org/10.3329/jbcs.v25i1.11780 Journal of Bangladesh Chemical Society, Vol. 25(2),97-100, 2012

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Section: Methodsmentioning

confidence: 98%

Characterization of the Eucalyptus ( E. Globulus) Leaves Oil

Khan

Khatun

Hossain

et al. 2012

J. Bangla. Chem. Soc.

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“…Lipid extraction of fetal tissue samples was determined as described previously (Rouser and Fleischer, 1967). Phospholipids were separated by ascending one-dimensional chromatography using the solvent system chloroform/methanol/water (65 :25 :4, v/v) as described previously (Rouser et al, 1966). The quantitative phosphorous determination, following two-dimensioned thin-layer chromatography was estimated according to Rouser et ai.…”

Section: Methodsmentioning

confidence: 99%

Chemical and biochemical studies in fetuses affected with nieman‐pick disease type A

et al. 1982

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Chemical and biochemical studies were performed on two unrelated fetuses affected with Niemann-Pick disease type A, following abortion at about the 19th week of gestation. Abortion was performed as a consequence of previous findings, in amniotic fluid cell cultures, that sphingomyelinase activity was completely absent. Phospholipid analyses of various organs of the fetuses, spleen and liver were the organs mostly affected. Interestingly enough considerable accumulation of sphingomyelin was found in the placenta. The brain was the only organ in which sphingomyelin storage could not be proved. In addition to sphingomyelin a slight accumulation of cholesterol was noticed. Deficiency of sphingomyelinase activity measured at pH 5.0 was the general characteristic of the affected tissues. It is concluded that the accumulation of sphingomyelin in various organs throughout the body of fetuses affected with Niemann-Pick disease is suggestive of the essential role of the enzyme sphingomyelinase and its biochemical maturation, even during the early stages of gestation.

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“…The detection of phthalic acid esters in soils, waters, and animal tissues is not too surprising, in view of the sensitivities of present day analytical methods, and the ease with which they are leached and volatilized from some formulations (1,4,38). Whether the levels reported are affecting the balance and development of Environmental Health Perspectives natural communities of organisms or not, is quite uncertain.…”

Section: Concluding Notementioning

confidence: 99%

Biodegradation of the Phthalates and Their Esters by Bacteria

Keyser¹,

Pujar²,

Eaton³

et al. 1976

Environmental Health Perspectives

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Recent studies on the biodegradation of phthalate esters in natural ecosystems, sewage, and laboratory cultures are reveiwed. There is ample evidence to demonstrate that bacteria are major elements in the biodegradative processes and that in most situations complete oxidation of the aromatic ring occurs; much less is known about the catabolism of the alcoholic moiety, e.g., 2-ethylhexanol. Evidence is presented to support catabolic pathways in pseudomonads and niicrococci that are initiated by successive hydrolyses of the diesters to give the phthalate anion.Thereafter a dioxygenase catalyzes the formation of 4,5-dihydro-4,5-dihydroxyphthalate, which is oxidized by an NAD-dependent dehydrogenase to give 4,5,dihydroxyphthalate. Protocatechuate, formed by decarboxylation of 4,5-dihydroxyphthalate, is the substrate for ring cleavage enzymes.Whereas flurorescent pseudomonads use the (J-ketoadipate pathway, the nonfluorescent strains and nhicrococci examined use a meta-cleavage (4,5-1 route. All the intermediates proposed have been accumulated by enzymes purified from Pseudomonas fluorescens. Isophthalate and terephthalate (anions) are readily used as carbon sources by aerobic bacteria, and preliminary evidence is consistent with catabolic routes for these isomers converging at the ring-cleavage substrate protocatechuate. Some possible effects and interactions of synthetic organic chemicals with the natural microflora, and the influence of other vectors, is discussed in relation to the maintenance of the ca'rbon cycle and environmental pollution.Phthalic acid esters (PAEs) have been synthesized on a massive scale for the last two to three decades, mainly for the formulation of plastics. In 1972 production was in excess of 109 lb/yr (1). A conference, sponsored by the National. Institute of Environmental Health Sciences four years ago, focused attention on the accumulation of phthalic acid esters in the environment, and how this might be potentially disadvantageous for the normal development of many life forms, including man. The papers presented were collectively published (1) and two excellent commentary reviews of the meeting appeared in Science (2) and Chemical and

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Laboratory contaminants in lipid chemistry: Detection by thin‐layer chromatography and infrared spectrophotometry and some procedures minimizing their occurrence

Cited by 42 publications

References 6 publications

Characterization of the Eucalyptus ( E. Globulus) Leaves Oil

Characterization of the Eucalyptus ( E. Globulus) Leaves Oil

Chemical and biochemical studies in fetuses affected with nieman‐pick disease type A

Biodegradation of the Phthalates and Their Esters by Bacteria

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