Laboratory Assays of the Effects of Oxfendazole on Biological Parameters of<i>Galleria mellonella</i>(Lepidoptera: Pyralidae)

Sugeçti, Serkan; Büyükgüzel, Ender; Büyükgüzel, Kemal

doi:10.18474/jes15-36.1

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…Twenty adult females were placed in glass jars (1000 ml) and provided a piece of old honeycomb with the diet (contents described below) for egg deposition and feeding of newly hatched larvae. Then, larvae were reared in 1-l glass jars (8 cm diameter, 18 cm high) (S ßis ßecam, Tuzla, Turkey) with an artificial diet in an incubator (ES 500; N€ uve, Ankara, Turkey) set at 30 AE 1°C, 65 AE 5% r.h., and L12:D12 photoperiod (B€ uy€ ukg€ uzel et al, 2010;Sugec ßti et al, 2016). The artificial diet content included the following: 420 g of wheat bran, 150 ml of filtered honey, 150 ml of glycerol (Merck, Darmstadt, Germany), 20 g of ground dark old honeycomb, and 30 ml of pure water (Bronskill, 1961).…”

Section: Insect Culturementioning

confidence: 99%

Biochemical and immune responses of model organism Galleria mellonella after infection with Escherichia coli

Sugeçti

2021

Entomologia Exp Applicata

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Galleria mellonella L. (Lepidoptera: Pyralidae) is used as an invertebrate model organism to study the virulence of bacteria pathogenic to humans. In this study, the oxidative effects of uropathogenic Escherichia coli (Migula) (Enterobacteriaceae) on the model organism were investigated. The levels of metabolic enzymes, non-enzymatic antioxidants, and biochemical parameters were measured in the hemolymph of G. mellonella larvae 2, 4, 6, and 8 h after E. coli inoculation. The results show that the levels of cell damage indicatorssuch as aspartate transferase, alanine transferase, and lactate dehydrogenaseincreased 8 h after E. coli infection. The levels of creatine kinase, alkaline phosphatase, and amylase increased in order to regulate the energy metabolism (negatively affected by the E. coli infection) and to reduce the biochemical effects of oxidative damage. Amounts of the non-enzymatic antioxidants uric acid, bilirubin, and albumin also increased after E. coli infection, whereas the amount of total protein was not affected. Altogether, E. coli infection caused oxidative stress and damage to the hemolymph of G. mellonella.

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Section: Insect Culturementioning

confidence: 99%

Biochemical and immune responses of model organism Galleria mellonella after infection with Escherichia coli

Sugeçti

2021

Entomologia Exp Applicata

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“…Ayrıca; parazit tedavisinde kullanılan antihelmintik antibiyotiklerin model organizmalar üzerinde toksikolojik etkileri ve doz belirlenmesinde önem arz etmektedir. [30] EntamoEba histolytica'nın türk‹ye prevelansı…”

Section: Amebiyazisunclassified

Entamoeba histolytica pathogenesis and molecular diagnostic methods

Sugeçti¹

2019

FNG & Bilim Tıp Dergisi

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Amebiasis is a protozoal disease of the intestines, caused by Entamoeba histolytica. The prevalence of E. histolytica is high in our country and in the world. E. histolytica is more common in undeveloped and developing countries. The diagnosis and treatment of this parasite is very important in preventing the spread of the disease. Similarities with Entamoeba dispar and E. histolytica cause constant misunderstanding in laboratory diagnoses. For this reason, fast and reliable laboratory diagnosis of parasites is crucial for human and animal health.Entamoeba histolytica pathogenesis and molecular diagnostic methods ÖZ Amebiyazis, Entamoeba histolytica'nın (E. histolytica) neden olduğu, bağırsakların protozoal bir hastalığıdır. E. histolytica prevalansı ülkemizde ve dünyada oldukça yüksektir. E. histolytica gelişmemiş ve gelişmekte olan ülkelerde daha sık görülmektedir. Bu parazitin tanısı ve tedavisi, hastalığın yayılmaması için oldukça önemlidir. Entamoeba dispar ve E. histolytica laboratuvar tanılarında benzerlikleri nedeniyle sürekli karıştırılan bir parazittir. Bu nedenle parazitin laboratuvar tanısın hızlı ve güvenilir olması insan ve hayvan sağlığı için oldukça önemlidir.

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“…For continuing insect culture, these newly hatched larvae were reared through seventh instar at the same artificial diets. The last larval instars were transferred into new jars to obtain pupal and adult stages (Büyükgüzel et al, 2010, Sugeçti et al 2016).…”

Section: Insect Culturementioning

confidence: 99%

“…Insect species belonging to the Lepitoptera order are particularly harmful for agricultural areas. For this reason, studies on chemical and biological control with these species have gained importance (Büyükgüzel 2001, Büyükgüzel and Kalender 2009, Büyükgüzel et al 2013, Sugeçti et al 2016, Sertçelik et al 2018. The use of insecticides against pest insects has also been increased in order to prevent these losses and to increase product yield.…”

Section: Introductionmentioning

confidence: 99%

“…In Galleria mellonella L. is used as an alternative invertebrate host to experimental animals which are able to determine the pathogenicity of certain chemicals, fungi or bacteria that infect humans and animals (Ramarao et al, 2012, Maguire et al, 2016. Moreover, this insect has been used as a model for evaluating the insecticidal properties of anthelmintic drugs with clinical prescription in recent years (Büyükgüzel and Kalender, 2007, Sugeçti et al, 2016, Sertçelik et al, 2018.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Oxfendazole on Some Biochemical Parameters in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet

Sugeçti

Büyükgüzel

2018

KSEJ

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Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations (0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes in metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest concentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl transferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL) activities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when compared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations because of its low acute toxicity to humans and other non-target organisms.

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Laboratory Assays of the Effects of Oxfendazole on Biological Parameters ofGalleria mellonella(Lepidoptera: Pyralidae)

Cited by 12 publications

References 25 publications

Biochemical and immune responses of model organism Galleria mellonella after infection with Escherichia coli

Biochemical and immune responses of model organism Galleria mellonella after infection with Escherichia coli

Entamoeba histolytica pathogenesis and molecular diagnostic methods

Effects of Oxfendazole on Some Biochemical Parameters in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet

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