Laboratory Approaches to the Diagnosis of Adenovirus Infection Depending on Clinical Manifestations

Terletskaia-Ladwig, Elena; Leinmüller, M.; Schneider, François; Meier, Simone Martina; Enders, Martin

doi:10.1007/s15010-007-6340-4

Cited by 16 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PCR has the highest sensitivity and specificity, whereas viral culture can be insensitive and take up to 21 days. 91,95 The treatment of adenovirus infections is supportive for mild disease; there are no approved antiviral drugs. Although often used in severe cases, ribavirin (a guanosine analogue) has not been shown to be effective in small clinical studies.…”

Section: Adenovirusmentioning

confidence: 99%

Influenza and Endemic Viral Pneumonia

Ramsey

Kumar

2013

Critical Care Clinics

View full text Add to dashboard Cite

Viruses are a common and important cause of severe community-acquired pneumonia, and may lead to severe respiratory disease and admission to the intensive care unit. Influenza is the most common virus associated with severe viral pneumonia, although other important causes include respiratory syncytial virus, adenovirus, metapneumonia virus, and coronaviruses. Viral pneumonias tend to have a seasonal predilection and are often preceded by a typical viral prodrome. This article focuses on severe influenza pneumonia, including the 2009 H1N1 pandemic, and briefly discusses other causes of severe respiratory disease of viral etiology.

show abstract

Section: Adenovirusmentioning

confidence: 99%

Influenza and Endemic Viral Pneumonia

Ramsey

Kumar

2013

Critical Care Clinics

View full text Add to dashboard Cite

show abstract

“…In children, viruses are the major causes of acute gastroenteritis [Parashar et al, ; Terletskaia‐Ladwig et al, ]. Rotavirus, adenovirus, astrovirus, norovirus, and sapovirus, have all been established as etiological agents of acute gastroenteritis in children [Hammond et al, ; Wilhelmi et al, ; Claas et al, ; Colomba et al, ; Unicef WHO, ; Churgay and Aftab, ; Chhabra et al, ; Payne et al, ].…”

Section: Introductionmentioning

confidence: 99%

“…Electron microscopy (EM) has been the gold standard diagnostic method for acute gastroenteritis, but this method requires a high concentration of viral particles per gram of stool, is labor‐intensive, [Kuypers et al, ; Wei et al, ] and is now infrequently used. Many laboratories use commercially available immunoassays for the diagnosis of rotavirus, norovirus, and adenovirus diseases; however, these tests are limited by their ability to detect a single viral pathogen at a time, poor sensitivity and specificity, and limited availability outside suspected outbreaks [de Bruin et al, ; Terletskaia‐Ladwig et al, ; Harris et al, ; Mans et al, ; Chhabra et al, ; Gautam et al, ]. Laboratories have been transitioning toward molecular‐based techniques like RT‐PCR for detection of etiologic viruses in patients with diarrheal illness.…”

Section: Introductionmentioning

confidence: 99%

Viral gastroenteritis in children in Colorado 2006–2009

Osborne

Montano

Robinson

et al. 2015

Journal of Medical Virology

View full text Add to dashboard Cite

Acute gastroenteritis accounts for a significant burden of medically attended illness in children under the age of five. For this study, four multiplex reverse transcription PCR assays were used to determine the incidence of adenovirus, astrovirus, coronavirus, norovirus GI and GII, rotavirus, and sapovirus in stool samples submitted for viral electron microscopy (EM) to the Children's Hospital Colorado. Of 1105 stool samples available, viral RNA/DNA was detected in 247 (26.2%) of 941 pediatric samples (median age = 2.97 years, 54% male) with 28 (3.0%) positive for more than one virus. Adenovirus, astrovirus, norovirus GI, norovirus GII, rotavirus, and sapovirus were detected in 95 (10.0%), 33 (3.5%), 8 (0.9%), 90 (9.6%), 49 (5.2%), and 2 (0.2%) of the pediatric samples, respectively. No coronaviruses were identified. Sequencing of norovirus positive samples indicated an outbreak of norovirus strain GII.4 in 2006 with evidence of numerous circulating strains. Multiple samples from the same immunocompromised patients demonstrated symptomatic shedding of norovirus for up to 32 weeks and astrovirus for 12 weeks. RT-PCR detected 99 of 111 (89%) adenovirus-positive samples versus 12 (11%) by EM, and 186 of 192 (97%) sapovirus/astrovirus/norovirus-positive samples versus 21 (11%) by EM. Noroviruses and adenoviruses are common causes of gastroenteritis in children. Immunocompromised patients can be infected with multiple viruses and shed viruses in their stools for prolonged periods. This data support the superiority of RT-PCR compared to EM for diagnosis of viral gastroenteritis.

show abstract

“…Cell culture and immunofluorescence antigen detection methods are less sensitive than polymerase chain reaction (PCR) in detecting HADV infections [Terletskaia-Ladwig et al, 2007]. Cell culture and immunofluorescence antigen detection methods are less sensitive than polymerase chain reaction (PCR) in detecting HADV infections [Terletskaia-Ladwig et al, 2007].…”

Section: Introductionmentioning

confidence: 99%

“…The gold standard for HADVs diagnosis is timeconsuming and laborious viral culture and identification. Cell culture and immunofluorescence antigen detection methods are less sensitive than polymerase chain reaction (PCR) in detecting HADV infections [Terletskaia-Ladwig et al, 2007]. Therefore, timely identification of adenoviral respiratory tract infections, as well as early identification of patients at risk of invasive HADV diseases, requires prompt detection of HADV DNA by nucleic acid amplification [Gray et al, 2007;Lee et al, 2010;Lion et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

Design and application of a real-time polymerase chain for the detection and subsequent characterization of respiratory adenoviral infections

Lee

et al. 2014

J. Med. Virol.

View full text Add to dashboard Cite

Human adenoviruses (HADVs) comprise at least 54 types and cause a wide spectrum of respiratory tract infections; early diagnosis and epidemiological monitoring of HADV infections requires a rapid and sensitive assay. The use of a real-time polymerase chain reaction (PCR) assay was evaluated with one set of in-house designed primers for respiratory adenoviral infections. The assay was first validated by detecting successfully 6 representative types and 100 clinical isolates. A concomitant prospective surveillance of viral aetiology using conventional cultures and PCR assays in 160 febrile children with acute respiratory tract symptoms was conducted between May 2010 and July 2011. Viral aetiologies were confirmed in 72 (45%) cases using conventional cultures, including 51 adenoviral infections. The concordance between the real-time PCR and culture was good (Kappa = 0.94), and two additional culture-negative adenovirus infections were identified. During the study period (January 2011), an adenoviral community epidemic occurred. Adenovirus B3 was the predominant type in this epidemic (69.8%), followed by C2 (5.7%), C1 (5.7%), C5 (1.9%), E4 (1.9%), C6 (1.9%), F41 (1.9%), and 4 unclassified species C (7.5%). Significantly prolonged duration of fever (>5 days), higher leukocyte counts, higher neutrophil counts, and higher C-reactive protein levels were in the adenoviral infected group (n = 53, P < 0.001), compared with the non-adenoviral infected group (n = 107). In conclusion, this in-house real-time PCR is capable of detecting adenoviral respiratory infections of various types in children; and patients with adenoviral aetiology suffered from more severe clinical manifestations.

show abstract

Laboratory Approaches to the Diagnosis of Adenovirus Infection Depending on Clinical Manifestations

Cited by 16 publications

References 17 publications

Influenza and Endemic Viral Pneumonia

Influenza and Endemic Viral Pneumonia

Viral gastroenteritis in children in Colorado 2006–2009

Design and application of a real-time polymerase chain for the detection and subsequent characterization of respiratory adenoviral infections

Contact Info

Product

Resources

About