1995
DOI: 10.1016/0733-5210(95)90046-2
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Laboratory and pilot plant extraction and purification of β-glucans from hull-less barley and oat brans

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Cited by 93 publications
(62 citation statements)
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“…The most traditional extraction methods involve several steps: (i) inactivation of endogenous enzymes in the grain, (ii) extractions with water or alkali solutions, (iii) removal of contaminating protein and starch using hydrolytic enzymes and/or selective adsorption, (iv) precipitation of b-glucans from the purified solutions with alcohol and freeze drying or alternatively drum or spray drying of the extracts (Bhatty, 1993(Bhatty, , 1995Cavallero, Empilli, Brighenti, & Stanca, 2002;Goering & Eslick, 1991;Wang, Lynch, & Goering, 1996). The resulting preparations may contain between 33% and 87% of b-glucans, however, they are expensive and have only been used in a few food products (Cavallero et al, 2002) or nutritional trials (Behall, Scholfield, & Hallfrisch, 2006;Delaney et al, 2003).…”
Section: Wet Extractionsmentioning
confidence: 99%
“…The most traditional extraction methods involve several steps: (i) inactivation of endogenous enzymes in the grain, (ii) extractions with water or alkali solutions, (iii) removal of contaminating protein and starch using hydrolytic enzymes and/or selective adsorption, (iv) precipitation of b-glucans from the purified solutions with alcohol and freeze drying or alternatively drum or spray drying of the extracts (Bhatty, 1993(Bhatty, , 1995Cavallero, Empilli, Brighenti, & Stanca, 2002;Goering & Eslick, 1991;Wang, Lynch, & Goering, 1996). The resulting preparations may contain between 33% and 87% of b-glucans, however, they are expensive and have only been used in a few food products (Cavallero et al, 2002) or nutritional trials (Behall, Scholfield, & Hallfrisch, 2006;Delaney et al, 2003).…”
Section: Wet Extractionsmentioning
confidence: 99%
“…Based on the new knowledge gathered in the last eight years on the detection of β-glucans in cereals, especially oats (Ren et al 2003;Johansson et al 2004;Tosh et al 2004) and barley (Zhang et al 2002;Burkus & Temelli 2003;Rimsten 2003), frequently applied methods are predominantly the enzymatic ones using α-amylase, 1,-3 and 1,4-β-d-glucanase, and β-d-glucosidase, cellulase, or lichenase (Genc et al 2001;Johansson et al 2004;Tosh et al 2004;Demirbas 2005;Lambo et al 2005;McCleary 2006) but also physical methods (centrifugation-dialysis filtration) (Lambo et al 2005) or extraction using NaOH (Bhatty 1995). The detection of β-glucans is carried out via HPLC (Pérez-Vendrel et al 1995) and HPEC-PAD (Johansson et al 2004), or via spectrophotometry in the UV (Demirbas 2005) and IR spectral regions (PN 01/01 Fungal glucan, 2001), respectively.…”
Section: Detection Methodsmentioning
confidence: 99%
“…The β-glucan yield or the % total recovered from barley bran and oat bran were 81% and 61% respectively. Later, the same approach was used by Bhatty [27] for pilot scale extraction. Although ethanol deactivation and extraction at higher pH with NaOH have been found to yield a high viscosity β-glucan gum, these processes are accompanied with a decrease in yield and an added risk of degradation of the β-glucan polymer [28].…”
Section: β-Glucanmentioning
confidence: 99%