Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries

Son, Weon‐Young; Tan, Seang Lin

doi:10.1093/humupd/dmq014

Cited by 97 publications

(88 citation statements)

References 89 publications

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“…Many factors affecting in vitro maturation rate have been reported. It has been demonstrated that priming of ovarian immature oocytes with follicle-stimulating hormone or human chorionic gonadotrophin prior to immature oocyte retrieval improves oocyte maturation rates and embryo quality as well as pregnancy rates in infertile women with polycystic ovaries or polycystic ovary syndrome [20,21]. The size of the follicles may also be important for the subsequent embryonic development, while the developmental competence of oocytes derived from the small antral follicles is not adversely affected by the presence of a dominant follicle [22].…”

Section: Discussionmentioning

confidence: 99%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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Purpose This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification. Methods A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2). Results Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p<0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p<0.05). Conclusion IVM procedure is more efficient when it is performed before oocyte vitrification.

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Section: Discussionmentioning

confidence: 99%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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“…The nuclear maturity of the collected oocytes was assessed under the dissecting microscope with high magnification (x80) using the spreading method [12]. If no germinal vesicle (GV) was observed in the oocyte cytoplasm, the cumulus masses were removed with hyaluronidase and mechanical pipetting was carried out after the oocyte retrieval was completed.…”

Section: In Vitro Maturationmentioning

confidence: 99%

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Son

Chung

Das

et al. 2012

J Assist Reprod Genet

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Purpose To analyze the fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle IVF in women with regular cycles. Methods Natural cycle IVF was performed in 28 patients who had normal ovaries, > 6 antral follicle counts and were less than 40 years old (n028 cycles). An hCG trigger of 10,000 IU was administered 36 h before oocyte collection when the diameter of the dominant follicle (DF) was over 12 mm. Oocytes were retrieved from DF as well as from the cohort of smaller follicles. Embryological aspects of the mature and immature oocytes retrieved from these cycles as well as the implantation and clinical pregnancy rates depending on the origin of the embryos transferred were evaluated. Result(s) Overall clinical pregnancy and implantation rates were 20.8 % and 6.7 %, respectively. There were no differences in in vitro maturation (IVM), fertilization and embryo development between immature oocytes retrieved with and without in vivo matured oocytes. However, the clinical and implantation rates in cycles with embryos produced from in vivo matured oocytes transferred were better than the cycles where only IVM embryos were transferred (30.8 %, 9.1 % vs. 9.1 %, 3.2 %). Conclusion(s) Although our results show that immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential.

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“…Several studies have reported pregnancies after IVM in PCOS with unstimulated cycles, also in cycles with FSH and hCG priming [2][3][4][5]. We believe that this is the first case report in which FSH priming was delayed until 13 th day of the cycle, when endometrial thickness reached 7 mm by endogenous estrogen.…”

Section: Discussionmentioning

confidence: 78%

“…In recent years, many improvements have been made in clinical and laboratory aspects of IVM treatment which have resulted in better pregnancy outcomes [4]. It was shown that pretreatment with FSH in the early days of folliculogenesis phase may increase the number of retrieved immature oocytes and/or maturation potential [5,6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Successful pregnancy following a novel endometrial preparation in a PCOS patient undergoing IVM: a case report

Eftekhar

Khalili

Aflatoonian

et al. 2012

J Assist Reprod Genet

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Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries

Abstract: The data from the meta-analyses suggests that the effect on clinical outcome of gonadotrophin priming of IVM still needs to be studied. In order to improve the IVM programs, it is essential to define not only the clinical aspects but also the laboratory and embryological aspects.

Cited by 97 publications

References 89 publications

In-vitro maturation of human oocytes: before or after vitrification?

In-vitro maturation of human oocytes: before or after vitrification?

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Successful pregnancy following a novel endometrial preparation in a PCOS patient undergoing IVM: a case report

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