Labial salivary gland biopsy is a reliable test for the diagnosis of primary and secondary amyloidosis. a prospective clinical and immunohistologic study in 59 patients

Hachulla, É.; Janin, Anne; Flipo, R.M.; Saı̈le, Rachid; Façon, Thierry; Bataille, D.; Vanhille, Philippe; Hatron, Pierre‐Yves; Devulder, B; Duquesnoy, B

doi:10.1002/art.1780360518

Cited by 92 publications

(32 citation statements)

References 13 publications

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“…Amyloid deposits in LSG usually appeared in a periductal distribution on CV or CR stained sections. These features were also found by Delgado and Mosqueda (10) and Hachulla et al (11). In the present study, the use of anti-AA antibody was helpful for confirmation of AA amyloidosis, particularly in cases with scant deposits of this substance.…”

Section: Discussionsupporting

confidence: 88%

Detection of AA-type amyloid protein in labial salivary glands

Sacsaquispe

Mayolo²,

Vicetti³

et al. 2011

Med Oral

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Objectives: Among the diverse forms of amyloidosis, secondary type is the most frequent one. Diagnosis of amyloid deposition is based on the identification of the fibrillary protein amyloid by means of Congo Red (CR) or crystal violet (CV) stains, but these techniques do not differentiate between the different types of amyloid fibrils. The aim of this study was to identify by immunofluorescence (IF) AA amyloid a pathological fibrillar low-molecularweight protein formed by cleavage of serum amyloid A (SAA) protein in labial salivary gland (LSG) biopsies from patients with secondary amyloidosis. Study design: 98 LSG were studied, 65 were from patients with secondary amyloidosis and 33 from subjects with chronic inflammatory diseases without evidence of this anomaly. All sections were stained with hematoxylin and eosin (H&E), CV, CR and IF using anti-AA antibodies. Positive and negative controls were used for all techniques.Results: CV and CR demonstrated that the amyloid substance was found mainly distributed periductally (93.8%), followed by periacinar and perivascular locations (p<0.001); however, the IF demonstrated that amyloid AA substance predominates in the periacinar area (73.8%), followed by periductal and perivascular locations (p<0.001). IF has a sensitivity of 83%, 100% of specificity, 100% of predictive positive value and 75% of predictive negative value.Conclusions: The results of this study confirm the efficacy of the LSG biopsy as a highly reliable method for diagnosis of secondary amyloidosis.

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Section: Discussionsupporting

confidence: 88%

Detection of AA-type amyloid protein in labial salivary glands

Sacsaquispe

Mayolo²,

Vicetti³

et al. 2011

Med Oral

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“…All LNs were free from cancer metastasis. The constitutive pattern of CCL21 expression was also investigated in noninflamed peripheral tissues, including two normal synovial tissues collected during lower limb amputation, two normal minor salivary glands obtained from individuals with suspected amyloidosis 28 (normal histology assessed by routine examination; Congo red-negative), one normal colon obtained during surgical resection for cancer at a minimum of 5 cm from the tumor (normal histology assessed by routine examination), and one normal skin sample obtained from mastectomy. All tissues were fixed in formalin and embedded in paraffin.…”

Section: Tissuesmentioning

confidence: 99%

CCL21 Expression Pattern of Human Secondary Lymphoid Organ Stroma Is Conserved in Inflammatory Lesions with Lymphoid Neogenesis

Manzo

Bugatti

Caporali

et al. 2007

The American Journal of Pathology

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CCL21 is a homeostatic lymphoid chemokine instrumental in the recruitment and organization of T cells and dendritic cells into lymphoid T areas. In human secondary lymphoid organs (SLOs), CCL21 is produced by cells distributed throughout the T zone, whereas high endothelial venules (HEVs) lack CCL21 mRNA. A critical question remains whether the development of ectopic lymphoid tissue (ELT) in chronic inflammation recapitulates the features of SLOs. Thus, we systematically investigated in situ the cellular sources of CCL21 in SLOs and ELTs in several human diseases characterized by lymphoid neogenesis. By in situ hybridization and the use of combinatorial cell markers, we show that CCL21-producing vessels in inflamed tissues systematically display typical markers of lymphatic vessels, whereas, as in SLOs, ectopic HEVs do not synthesize detectable levels of CCL21. We also provide first-time evidence that a common pattern of CCL21 expression by CD45-nega-

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“…Biopsy of the minor LSG is a sensitive, easily performed, and reproducible technique for amyloidosis diagnosis (6). In our patient, no amyloid deposits were found in the biopsied tissues or in LSG.…”

Section: Lambda Light Chain Deposition Disease Presenting As Sicca Symentioning

confidence: 46%