LABELING OF ERYTHROCYTES<i>IN VITRO</i>WITH RADIOIODINE-TAGGED<i>l</i>-TRIIODOTHYRONINE AS AN INDEX OF THYROID FUNCTION: AN IMPROVED HEMATOCRIT CORRECTION*

Adams, Ralph; Specht, Norman W.; Woodwar, Divor

doi:10.1210/jcem-20-10-1366

Cited by 18 publications

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“…It is known that T3 RCU test in vitro as an index of thyroid function changes remarkably with variations in hematocrit (Adams et al, 1960;Barrett et al, 1960). However, this factor has not been examined in the present study.…”

Section: Correlation Between the Free T4 Index And The Red-cell T3mentioning

confidence: 99%

Determination of Triiodothyronine in Red Blood Cells by Radioimmunoassay

et al. 1976

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Section: Correlation Between the Free T4 Index And The Red-cell T3mentioning

confidence: 99%

Determination of Triiodothyronine in Red Blood Cells by Radioimmunoassay

et al. 1976

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“…None of the patients were pregnant or on ovulation suppressant or salicylate therapy. The results are given in terms of the triiodothyronine-binding coefficient for Sephadex since this gives values which can readily be compared with 8 those obtained in similar tests using the red cells (Adams et al, 1960). The values obtained in the euthyroid patients are not quite normally distributed; the normal range has been taken to be 0 050 to 0-100.…”

Section: Resultsmentioning

confidence: 99%

“…CALCULATION OF REsuLTs The distribution of a small tracer dose of radioactive triiodothyronine added to serum may be described by expressing the quantity of 'free' triiodothyronine as a fraction of the protein-bound triiodothyronine: this is the 'T3-binding coefficient' (Adams, Specht, and Woodward, 1960) in the Sephadexserum system under the given experimental conditions. Counts in last 4 ml.…”

Section: Methodsmentioning

confidence: 99%

Use of Sephadex column chromatography in the assessment of thyroid status

Gimlette

1967

J Clin Pathol

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SYNOPSIS A method is described for assessing thyroid status using Sephadex column chromatography of serum samples with added 1251 triiodothyronine. The method is simple and inexpensive. It gave a correct diagnostic result in 97% of 258 euthyroid, 90% of 80 hyperthyroid, 78% of 59 hypothyroid, and 67 % of 50 euthyroid, ill patients.The principle of the method and its limitations are briefly discussed.Since the red cell uptake of 131I-labelled triiodo-

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“…2. ) In the majority the triiodothyronine binding coefficient (Adams, Specht, and Woodward, 1960) was also estimated by the method of Hamolsky, Golodetz, and Freedberg (1959) and the results are compared (Fig. 3 patients the distinction between normal and hyperthyroid (more than 0-80% dialysable in 16 hours or T3 binding coefficient over 0-115) was made a little more successfully by dialysable thyroxine than by tri-iodothyronine binding coefficient; the differentiation of hypothyroidism was less satisfactory by both methods.…”

Section: Resultsmentioning

confidence: 99%

A simple technique using `dialysable' thyroxine for assessment of thyroid status

Gimlette

1964

Journal of Clinical Pathology

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SYNOPSIS A simple method for assessing thyroid status is described in which the percentage of radioactive thyroxine dialysed through a semi-permeable membrane between plasma samples is estimated. The results compare favourably with other tests in vitro.Two factors which influence thyroid status are the plasma content of thyroid hormone (mainly thyroxine) and the binding capacity of the thyroxinebinding protein; these dispose the quantity of thyroxine available to the tissues. Unfortunately neither protein-bound iodine or plasma thyroxine (Ekins, 1960) nor thyroxine-binding protein capacity (Robbins, 1956) are very simple to estimate as a routine. The quantity of thyroxine not bound to protein, or available, is too small to be estimated directly. By the addition of a small trace of 1311-labelled thyroxine to plasma it is possible to estimate the proportion of total thyroxine which is free, or available, by dialysis from plasma to buffer solution (Sterling and Hegedus, 1962) and this has been found to give a good indication of thyroid status. The technique is, however, somewhat exacting since the proportion of thyroxine 1311 which is dialysable from plasma to buffer is exceedingly small, owing to the high affinity of thyroxine-binding protein for thyroxine. It is easier to estimate the proportion of a trace of 1311 thyroxine which is dialysable in a given time across a semi-permeable membrane separating two plasma samples (Christensen, 1959

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LABELING OF ERYTHROCYTESIN VITROWITH RADIOIODINE-TAGGEDl-TRIIODOTHYRONINE AS AN INDEX OF THYROID FUNCTION: AN IMPROVED HEMATOCRIT CORRECTION*

Cited by 18 publications

References 0 publications

Determination of Triiodothyronine in Red Blood Cells by Radioimmunoassay

Determination of Triiodothyronine in Red Blood Cells by Radioimmunoassay

Use of Sephadex column chromatography in the assessment of thyroid status

A simple technique using `dialysable' thyroxine for assessment of thyroid status

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