Labeling-free fluorescent detection of DNA hybridization through FRET from pyrene excimer to DNA intercalator SYBR green I

Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection

“…Over the last decade, label-free biosensors have been used for rapid and sensitive bimolecular detection in the monitoring of the environment, controlling of food products and their use in the medical diagnostics [6,7,8,9,10,11]. Among them, the label-free biosensor based on the hybridization between capture DNA and target DNA enables direct, sensitive and rapid detection of DNA without target amplification [12,13,14]. Various label-free sensing mechanisms are developed for DNA detection such as surface plasmon resonance (SPR), electrochemistry, reflectance spectroscopy, infrared spectroscopy and surface enhanced Raman spectroscopy (SERS) [15,16,17,18,19].…”

Pyrene Derivative Emitting Red or near-Infrared Light with Monomer/Excimer Conversion and Its Application to Ratiometric Detection of Hypochlorite

“…Pyrene is also used frequently as fluorophore with a unique luminescence pattern. , In dilute solutions (molecularly dissolved state), upon UV excitation, emission from the pyrene monomer (λ ≈ 380–410 nm) can be observed; at increased concentrations (aggregated state), the red-shifted emission band appears (λ ≈ 450–500 nm), which is attributed to the excimer formed when an excited-state molecule is brought into close proximity with another ground-state molecule. Thanks to the predictability of the two luminescence signals, pyrene moieties have been introduced in the design of numerous ratiometric sensors as reporters and other functional materials. − The ratiometric mode of pyrene-involved sensing, which involves the synchronous measurement of two fluorescence signals at different wavelengths followed by the calculation of their intensity ratio, is an elegant approach for achieving ratiometric sensing by simply using a single fluorophore molecule and is aimed to overcome unfavorable effects of the turn-on mode sensing. In recent years, some new pyrene-derivative chromophores emitting red fluorescence and none-pyrene chromophores with excimer-forming capability have been synthesized. , For example, Ramaiah’s group created a red fluorescence sensor based on pyrene and Cy fluorophore and used it in the detection of cyanide .…”

“…Thanks to the predictability of the two luminescence signals, pyrene moieties have been introduced in the design of numerous ratiometric sensors as reporters and other functional 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 3 materials [17][18][19][20][21][22][23][24][25][26][27][28] . The ratiometric mode of pyrene-involved sensing, which involves the synchronous measurement of two fluorescence signals at different wavelengths followed by calculation of their intensity ratio, is an elegant approach for achieving ratiometric sensing by simply using a single fluorophore molecule and is aimed to overcome unfavorable effects of the turn-on mode sensing.…”

A upconversion luminescene biosensor based on dual-signal amplification for the detection of short DNA species of c-erbB-2 oncogene

“…Conclusively, the as-obtained relative standard deviation (RSD) tested in the uniform plate and different plates are 2.82% and 3.56%, respectively, which shows that the developed biosensor exhibited good reproducibility and acceptable stability. Recently, many researches have focused on the different signal amplification techniques to detect DNA, and the reported corresponding LODs are 10 nM 32 , 10 pM 33 , 0.167 pM 34 , 0.1 pM 35 , and 30 fM 36 respectively (More details shown as in Supplementary Table S1 ). Compared with the above research, our proposed UCL biosensor based on dual signal amplification of ExoIII-assisted target cycles and long-range self-assembly DNA concatamers shows high sensitivity and stability.…”