A upconversion luminescene biosensor based on dual-signal amplification for the detection of short DNA species of c-erbB-2 oncogene

Lan, Jianming; Liu, Yingxin; Li, Li; Wen, Fadi; Wu, Fang; Han, Zhizhong; Sun, Wei‐Ming; Li, Chunyan; Chen, Jinghua

doi:10.1038/srep24813

Cited by 9 publications

(2 citation statements)

References 37 publications

(37 reference statements)

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“…Furthermore, NaYF 4 :Yb 3+ /Er 3+ luminescent labels were combined with the amplification effects of exonuclease III (Exo III) assisted target recycling and DNA concatemers for the detection of the c-erbB2 oncogene. 77 Here, signal amplification was achieved as a consequence of hybridization, degradation, and further hybridization in the presence of Exo III. Furthermore, the presence of AP1 and AP2 (auxiliary probes) triggered the development of super sandwich DNA concatemers to generate an amplified up-conversion luminescence signal (excitation at 980 nm) towards c-erbB2 oncogene (detection limit of 40 aM).…”

Section: Biosensors and Nano-biosensors For Breast Cancer Diagnosismentioning

confidence: 99%

Recent advances in biosensing approaches for point-of-care breast cancer diagnostics: challenges and future prospects

et al. 2021

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Section: Biosensors and Nano-biosensors For Breast Cancer Diagnosismentioning

confidence: 99%

Recent advances in biosensing approaches for point-of-care breast cancer diagnostics: challenges and future prospects

et al. 2021

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“…Typically, they can be excited by near infrared (NIR) light which avoids biofluorescence background212223. In addition, UCNPs have large anti-stokes emissions (>300 nm), good photo-stability, lower cytotoxicity and narrow multi-emission bands etc.…”

mentioning

confidence: 99%

Accurate Quantitative Sensing of Intracellular pH based on Self-ratiometric Upconversion Luminescent Nanoprobe

Zuo

Zhang

et al. 2016

Sci Rep

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Accurate quantitation of intracellular pH (pHi) is of great importance in revealing the cellular activities and early warning of diseases. A series of fluorescence-based nano-bioprobes composed of different nanoparticles or/and dye pairs have already been developed for pHi sensing. Till now, biological auto-fluorescence background upon UV-Vis excitation and severe photo-bleaching of dyes are the two main factors impeding the accurate quantitative detection of pHi. Herein, we have developed a self-ratiometric luminescence nanoprobe based on förster resonant energy transfer (FRET) for probing pHi, in which pH-sensitive fluorescein isothiocyanate (FITC) and upconversion nanoparticles (UCNPs) were served as energy acceptor and donor, respectively. Under 980 nm excitation, upconversion emission bands at 475 nm and 645 nm of NaYF4:Yb3+, Tm3+ UCNPs were used as pHi response and self-ratiometric reference signal, respectively. This direct quantitative sensing approach has circumvented the traditional software-based subsequent processing of images which may lead to relatively large uncertainty of the results. Due to efficient FRET and fluorescence background free, a highly-sensitive and accurate sensing has been achieved, featured by 3.56 per unit change in pHi value 3.0–7.0 with deviation less than 0.43. This approach shall facilitate the researches in pHi related areas and development of the intracellular drug delivery systems.

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A Universal Upconversion Sensing Platform for the Sensitive Detection of Tumour‐Related ncRNA through an Exo III‐Assisted Cycling Amplification Strategy

Zhang

Yang

et al. 2018

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Here, a sensitive and universal noncoding RNA (ncRNA) upconversion sensing nanoplatform is developed. Gold nanoparticles bearing one hairpin DNA (Hp) molecule are conjugated to the linker DNA modified NaYF :Yb, Er@NaYF upconversion nanoparticles by DNA hybridization, leading to quenching of the upconversion emission through fluorescence resonance energy transfer. A signal DNA (SDNA) sequence is designed to open Hp, recovering the upconversion emission. To achieve universality and high sensitivity of the nanoprobe, an exonuclease III (Exo III)-assisted cycling amplification strategy is introduced. A multifunctional hairpin DNA (mHp) containing ncRNA recognition sequence and SDNA sequence is designed to recognize ncRNA and trigger Exo III as a biocatalyst to stepwise disintegrate itself, releasing both ncRNA and SDNA. The released ncRNA can be reused to release more SDNA, which greatly improves the sensing sensitivity. By changing the recognition portion of mHp, various ncRNA can be detected. The sensitive detection of both homeobox (HOX) transcript antisense RNA segment and miR-21 is achieved with this novel strategy, even in human serum, indicating the universality and sensitivity of the proposed strategy. Additionally, the expression level of miR-21 in human breast cancer cell (MCF-7) lysate is successfully measured, suggesting its potential in clinical diagnosis.

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A upconversion luminescene biosensor based on dual-signal amplification for the detection of short DNA species of c-erbB-2 oncogene

Cited by 9 publications

References 37 publications

Recent advances in biosensing approaches for point-of-care breast cancer diagnostics: challenges and future prospects

Recent advances in biosensing approaches for point-of-care breast cancer diagnostics: challenges and future prospects

Accurate Quantitative Sensing of Intracellular pH based on Self-ratiometric Upconversion Luminescent Nanoprobe

A Universal Upconversion Sensing Platform for the Sensitive Detection of Tumour‐Related ncRNA through an Exo III‐Assisted Cycling Amplification Strategy

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