Labeled extra virgin olive oil as food supplement; phenolic compounds in oils from some autochthonous Croatian olives

Brala, Cvijeta Jakobušić; Benčić, Đani; Šindrak, Zoran; Barbarić, Monika; Uršić, Stanko

doi:10.3989/gya.0228151

Cited by 3 publications

(6 citation statements)

References 51 publications

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“…All analysed compounds were present in low concentrations, below 10 mg/kg. Similar values were previously obtained for other Croatian olive oils [ 69 , 79 ].…”

Section: Resultssupporting

confidence: 90%

“…Five phenolic compounds—the simple polyphenols HTyr, tyrosol (Tyr), and cinnamic acid and the flavonoids apigenin and lignan pinoresinol—were determined by HPLC chromatography using a DAD detector following the previously described procedure [ 69 , 70 ] ( Figure S3 ). Prior to HPLC analysis, polyphenols were extracted from olive oil by US-LLE using methanol and hexane [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

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Extra Virgin Olive Oil Secoiridoids Modulate the Metabolic Activity of Dacarbazine Pre-Treated and Treatment-Naive Melanoma Cells

et al. 2022

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Nowadays, many individuals, whether healthy or diagnosed with disease, tend to expose themselves to various easily accessible natural products in hopes of benefiting their health and well-being. Mediterranean populations have traditionally used olive oil not only in nutrition but also in cosmetics, including skincare. In this study, the phenolic profile—composed of twelve compounds altogether, including the secoiridoids oleocanthal (OCAL) and oleacein (OCEIN)—of extra virgin olive oil (EVOO) from autochthonous cultivars from Croatia was determined using 1H qNMR spectroscopy and HPLC-DAD analysis, and its biological activity was investigated in melanoma cell lines. The EVOO with the highest OCEIN content had the strongest anti-cancer activity in A375 melanoma cells and the least toxic effect on the non-cancerous keratocyte cell line (HaCaT). On the other hand, pure OCAL was shown to be more effective and safer than pure OCEIN. Post-treatment with any of the EVOO phenolic extracts (EVOO-PEs) enhanced the anti-cancer effect of the anti-cancerous drug dacarbazine (DTIC) applied in pre-treatment, while they did not compromise the viability of non-cancerous cells. The metastatic melanoma A375M cell line was almost unresponsive to the EVOO-PEs themselves, as well as to pure OCEIN and OCAL. Our results demonstrate that olive oils and/or their compounds may have a potentially beneficial effect on melanoma treatment. However, their usage can be detrimental or futile, especially in healthy cells, due to inadequately applied concentrations/combinations or the presence of resistant cells.

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“…All analysed compounds were present in low concentrations, below 10 mg/kg. Similar values were previously obtained for other Croatian olive oils [ 69 , 79 ].…”

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Extra Virgin Olive Oil Secoiridoids Modulate the Metabolic Activity of Dacarbazine Pre-Treated and Treatment-Naive Melanoma Cells

et al. 2022

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“…The determined concentrations of hydroxytyrosol in VOO and VOO-Sea, that amount to 18.2 mg/kg and 12.9 mg/kg, respectively, are generally in the range similar to the values in some Spanish (6.9–72.7 mg/kg of oil) or Italian EVOOs (0.85–36.7 mg/kg of oil) [ 17 , 19 , 20 ], and are even higher than those previously published for Oblica variety (6.2–11.3 mg/kg of oil) [ 11 , 21 , 22 ]. Furthermore, the tyrosol concentration determined in VOO and VOO-Sea (8.10 mg/kg and 7.20 mg/kg respectively) is very similar to the values in the same literature.…”

Section: Resultssupporting

confidence: 82%

“…A HPLC-DAD analysis was performed essentially by applying the procedure [ 11 ]. The HPLC system (Perkin Elmer Series 200, Shelton, CT, USA) equipped with diode array detector (DAD) was used.…”

Section: Methodsmentioning

confidence: 99%

Antique Traditional Practice: Phenolic Profile of Virgin Olive Oil Obtained from Fruits Stored in Seawater

Torić

Barbarić

Uršić

et al. 2020

Foods

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Virgin olive oil (VOO) is a functional food specific to the Mediterranean diet and related to human health, especially as a protector of cardiovascular health, in the prevention of several types of cancers, and in modification of immune and inflammatory response. Phenolic compounds have central importance for these extraordinary health benefits. In the production of high-quality olive oils, it is very important to process freshly picked olives and avoid any storage of fruits. However, in Croatia there is a very traditional and environmentally friendly method of olive oil production, where olive fruits are stored in seawater for some time prior to processing. This practice is also notable nowadays since there are people who prefer the characteristic flavor of the “seawater olive oil”, although some people argue against its quality and biomedical relevance. In this study, the phenolic contents of VOO prepared from the immediately processed fresh olives and olives processed after storage in seawater were compared with the use of high-performance liquid chromatography-mass spectrometry (HPLC-MS) and spectrophotometric analysis. The results suggest that “seawater olive oil” should be considered as a safe food of biomedical relevance, as it still contains a significant proportion of important phenolics like hydroxytyrosol, tyrosol and oleacein (e.g., 63.2% of total phenols in comparison to VOO).

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“…Following the procedure, according to Jakobušić Brala et al [22] and Owen et al [23], an HPLC-DAD analysis was performed. HPLC analyses were performed in a Perkin Elmer Series 200 system (USA) with diode array detector (DAD) at 25 • C. The column used in the analysis was C18 Restek column (5 µm, 250 × 4.0 mm).…”

Section: Hplc-dad Analysismentioning

confidence: 99%

Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells

et al. 2020

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The roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenolic profiles of extra virgin olive oils (EVOOs) from different autochthonous cultivars from Croatia (Oblica, Bjelica, Buža, Žižolera) and investigated the biological effect of EVOO phenolic extracts (EVOO-PEs) on human cervical (HeLa) and human colon (SW48) cancer cell lines alone and in combination with cisplatin (cDDP), carboplatin (CBP), 5-fluorouracil (5-FU) and irinotecan. The quantitative evaluation of olive oil polyphenols was performed by HPLC-DAD and spectrophotometric analysis. The biological effect of EVOO-PEs alone and in combination with anticancer drugs was measured by MTT assay. Analysed EVOO-PEs differ in phenolic profile and inhibited HeLa and SW48 cells in a dose-dependent manner. Further, it is shown that EVOO-PEs (Oblica-Sea, Buža and Žižolera), in combination with anticancer drugs, increase the metabolic activity of HeLa and SW48 cells and have a protective role. These data imply careful consummation of olive oil during chemotherapy of cancer patients.

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Labeled extra virgin olive oil as food supplement; phenolic compounds in oils from some autochthonous Croatian olives

Cited by 3 publications

References 51 publications

Extra Virgin Olive Oil Secoiridoids Modulate the Metabolic Activity of Dacarbazine Pre-Treated and Treatment-Naive Melanoma Cells

Extra Virgin Olive Oil Secoiridoids Modulate the Metabolic Activity of Dacarbazine Pre-Treated and Treatment-Naive Melanoma Cells

Antique Traditional Practice: Phenolic Profile of Virgin Olive Oil Obtained from Fruits Stored in Seawater

Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells

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