Label-Free Quantification of Anti-TNF-α in Patients Treated with Adalimumab Using an Optical Biosensor

Abstract

This study describes the development of an immunosensory label-free quantification methodology based on surface plasmon resonance (SPR) and its applicability in measuring/evaluating therapeutic drug monitoring (TDM) of anti-TNF-α monoclonal antibody (adalimumab) in rheumatoid arthritis (RA) patients. The experimental parameters evaluated in this study were immobilising ligands by pre-concentration assays, sensor surface regeneration, ascertaining the method’s sensitivity and correlating the results from quantifying plasma samples by ELISA immunoassay. The results showed that TNF-α quantification values (in RU) were significantly different when comparing patients (~50–250 RU) to controls (~10–20 RU). Likewise, there was 0.97 correlation for patients and 0.91 for healthy volunteers using SPR and ELISA comparison methodologies. SPR immunosensory detection provided a precise, sensitive strategy, along with real-time determination, for quantifying adalimumab, having great potential for clinical routine regarding TDM.

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor

“…SPR sensors have been widely applied for bioanalytes detection such as protein, small molecules, and miRNA. Many microfluidics and chips are currently integrated with SPR, − these advances in technology have made it possible to isolate exosome populations with greater sensitivity. Im et al, for example, established a nanoplasmonic assay that made it possible to profile exosomes according to membrane proteins and proteins found in the lysates .…”

Optical Biosensors for Therapeutic Drug Monitoring

“…However, these techniques require trained personnel and a clinical laboratory with the necessary equipment and reagents. Such techniques include radioimmunoassays (RIA), enzyme-linked immunosorbent assays (ELISA), and fluorescence polarization immunoassays (FPIA), which have been used for quantifying antibiotics, anticancer/antineoplastic, anti-arrhythmic, and biological drugs [33][34][35][36][37][38].…”

“…Such is the case for the measurement of the plasma levels of antibodies and anti-drug antibodies for infliximab (IFX) [189] and anti-TNF-alpha [35]. For the determination of these serum levels, sensor surfaces were used for activation via amino coupling sulfo-N-hydroxysuccinimide/1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (sulfo-NHS/EDC).…”

Determination of Tyrosine Kinase Inhibitors via Capillary Electrophoresis with Tandem Mass Spectrometry and Online Stacking Preconcentration

“…However, these techniques require trained personnel and a clinical laboratory with the necessary equipment and reagents. Such techniques include radioimmunoassays, enzyme-linked immunosorbent assays (known as ELISA), and fluorescence polarization immunoassays, which have been used for quantifying antibiotics as well as anticancer/antineoplastic, antiarrhythmic, and biological drugs [ 6 , 10 ].…”