Determination of Tyrosine Kinase Inhibitors via Capillary Electrophoresis with Tandem Mass Spectrometry and Online Stacking Preconcentration

Abstract

Capillary electrophoresis connected with tandem mass spectrometry was employed for the development of a method for determination of various tyrosine kinase inhibitors in plasma samples. A stacking online preconcentration with a 120 cm-long capillary was used for the determination of bosutinib, dasatinib, canertinib, and erlotinib at physiologically relevant concentrations. The optimization included both capillary electrophoresis and mass spectrometry steps. Under optimal conditions, 50 mM formic acid pH 2.5, an injection time of 120 s, and an optimized mass spectrometry set-up (as sheath liquid composition 75:24.9:0.1 (v/v) methanol, water, formic acid, and appropriate conditions for ion transitions), LODs in a range of 3.9–23.0 nmol.L−1 were observed. The method was validated in terms of linearity, limit of detection, limit of quantification, repeatability of migration times and peak area, and recovery using plasma as a matrix for analytes. The results showed that this method has great promise for use in many analytical tasks, e.g., therapeutic drug monitoring.

Separation of tamsulosin enantiomers by capillary electrophoresis with tandem mass spectrometry and online stacking preconcentration

“…An increase in injection time with dilution of a sample in 50% (v/v) methanol-BGE was applied to decrease the LOD values via so-called stacking online preconcentration. 37,38 Here, the differences in electric eld strength in adjoining zones lead to the slowdown of analytes and their preconcentration. To obtain the lowest LOD values, the injection time was varied between 10 and 60 s (Fig.…”

Capillary Electrophoresis: A Three-Year Literature Review

“…CE techniques have found extensive applications in the impurity profiling and assay of numerous small molecules associated with medical applications. These include various types of drugs such as antipsychotics, anti-inflammatories, anticancer agents, narcotic analgesics, or anesthetics, as well as active ingredients in herbal medicines . Typically, nonchiral drugs and their related substances/impurities were predominantly determined with CZE, while chiral separations and optical purity testing were mostly resolved with EKC-based methods (as summarized in the previous Enantiomers section).…”