Label-free photoacoustic microscopy of myocardial sheet architecture

Zhang, Chi; Cheng, Ya-Jian; Chen, Junjie; Wickline, Samuel A.; Wang, Lihong V.

doi:10.1117/1.jbo.17.6.060506

Cited by 39 publications

(34 citation statements)

References 13 publications

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“…It has been employed to image microvasculature, melanoma tumors, and cell mitochondria through endogenous chromophores such as hemoglobin, melanin, and cytochrome C [7][8][9]. In a clinical setting, these chromophores can potentially aid in tumor margining and characterization of portwine stains [10].…”

Section: Introductionmentioning

confidence: 99%

Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Mattison

Shelton

Maxson

et al. 2013

Biomed. Opt. Express

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A four dimensional data set of the cardiac cycle of a zebrafish embryo was acquired using postacquisition synchronization of real time photoacoustic b-scans. Utilizing an off-axis photoacoustic microscopy (OA-PAM) setup, we have expanded upon our previous work with OA-PAM to develop a system that can sustain 100 kHz line rates while demodulating the bipolar photoacoustic signal in real-time. Real-time processing was accomplished by quadrature demodulation on a Field Programmable Gate Array (FPGA) in line with the signal digitizer. Simulated data acquisition verified the system is capable of real-time processing up to a line rate of 1 MHz. Galvanometer-scanning of the excitation laser inside the focus of the ultrasonic transducer enables real data acquisition of a 200 by 200 by 200 pixel, volumetric data set across a 2 millimeter field of view at a rate of 2.5 Hz.

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Section: Introductionmentioning

confidence: 99%

Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Mattison

Shelton

Maxson

et al. 2013

Biomed. Opt. Express

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“…Historically, label-free PAM has been successfully applied to in vivo imaging of hemoglobin and melanin, two major sources of endogenous optical absorption in biological tissue in the visible spectral range. Recently, additional photoacoustic contrasts have been demonstrated by exciting myoglobin 1 and bilirubin 2 with visible illumination, DNA and RNA in nuclei 3 with ultraviolet (UV) illumination, and water 4 and lipid 5 with near-infrared illumination. In fact, PAM can potentially image any molecule that has sufficient absorption at specific wavelengths.…”

mentioning

confidence: 99%

Label-free photoacoustic microscopy of cytochromes

et al. 2013

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“…Recently, by blunt dissection of the epicardium, or epimysium, that surrounds the ventricular mass, the cardiac myofibers forming the left ventricle (LV) had been reported to be organized in branching layers separated by cleavages, referred to as the "sheet architecture" [8,9].…”

Section: Introductionmentioning

confidence: 99%

Histological architecture of cardiac myofibers composing the left ventricle of murine heart

Gouda¹,

Elewa²,

Selim³

2015

J Histol Histopathol

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Background: Despite the fact that the exact architecture and orientation of left ventricular myocardial fibers are critical to cardiac functions either in health or disease, it is still debated. Aim of the work: Histological demonstration of the transverse alignment of the myofibers making the left ventricular mass was applied to validate different anatomical speculations of cardiac myofibers organization; mainly Streeter's conjecture and the ventricular myocardial band model of Torrent-Guasp. Material and methods: Six healthy adult male C57BL/6N mice were utilized. Their hearts were separated into atria and ventricles. Then, both ventricles were divided into four levels [base, upper mid, lower mid and apex]. Paraffin step-serial transverse sections were stained with H&E and Masson's trichrome stains. Morphometrical measurements of the thickness of the anterior, posterior, and lateral walls of the left ventricle (LV) as well as the inter-ventricular septal wall were realized with ImageJ software. For data analyses among the four levels of LV, Scheffé's method was applied for multiple comparisons when a significant difference was observed by Kruskal-Wallis test (p<0.05). Results: Examination of the LV from its base to its apex revealed obvious changes in the thickness of its walls and inter-ventricular septum as well as in its cavity shape. Interestingly, the myocardial fibers showed different running patterns [longitudinal, oblique and circular] among different levels as well as within the same level from sub-epicardial to the endocardial region. Throughout all levels, the subepicardial myofibers showed longitudinal orientation while that of the intermediate wall revealed either oblique [in upper mid-level] or circular orientations in other levels. The sub-endocardial fibers were mostly longitudinal with the presence of some circular fibers in the base and upper-mid regions. Masson's trichrome sections revealed a trivial amount of collagen fibers just around each individual myocyte without any bundle formation. Conclusion:The presence of different running patterns of the myocardial fibers among different levels of LV as well as within the same level indicates multiple rolling of the myocardial fibers. Thus, we suggested that the band model by Torrent-Guasp accounts for the patterns of myocardial fiber architecture forming the left ventricle.

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Label-free photoacoustic microscopy of myocardial sheet architecture

Cited by 39 publications

References 13 publications

Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Label-free photoacoustic microscopy of cytochromes

Histological architecture of cardiac myofibers composing the left ventricle of murine heart

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