Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Mattison, Scott P.; Shelton, Ryan L.; Maxson, Ryan; Applegate, Brian E.

doi:10.1364/boe.4.001451

Cited by 13 publications

(11 citation statements)

References 31 publications

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“…This discrepancy is mainly caused by the unflatten MEMS scanner surface and mismatch of reflective indexes among air, glass and water in the light path. As shown in Figure C, the FWHM of the Gaussian‐fitted axial profile of a typical depth‐resolved PA signal is ~104 μm, which agrees well with the theoretical axial resolution (105 μm) calculated using the given center frequency and bandwidth of the transducer .…”

Section: Resultssupporting

confidence: 83%

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Guo

Chen

et al. 2018

Journal of Biophotonics

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Brain imaging is an important technique in cognitive neuroscience. In this article, we designed a stereotaxic-apparatus-compatible photoacoustic microscope for the studies of rat cortical hemodynamics. Compared with existing optical resolution photoacoustic microscopy (ORPAM) systems, the probe owns feature of fast, light and miniature. In this microscope, we integrated a miniaturized ultrasound transducer with a center frequency of 10 MHz to detect photoacoustic signals and a 2-dimensional (2D) microelectromechanical system (MEMS) scanner to achieve raster scanning of the optical focus. Based on phantom evaluation, this imaging probe has a high lateral resolution of 3.8 μm and an effective imaging domain of 2 × 2 mm . Different from conventional ORPAMs, combining with standard stereotaxic apparatus enables broad studies of rodent brains without any motion artifact. To show its capability, we successfully captured red blood cell flow in the capillary, monitored the vascular changes during bleeding and blood infusion and visualized cortical hemodynamics induced by middle cerebral artery occlusion.

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Section: Resultssupporting

confidence: 83%

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Guo

Chen

et al. 2018

Journal of Biophotonics

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“…successfully tracked genetically expressed fluorescence proteins in the exocrine pancreas of the adult zebrafish via dual-wavelength PAI [28]. Applegate and Zhou fabricated new acoustic transducers which were utilized to detect the photoacoustic signals of the zebrafish heart with a high temporal resolution [29,30]. Li, for the first time, applied the transmissionmode ORPAM to visualize the microvascular network of the zebrafish larvae at a lateral resolution of 6 μm [7].…”

Section: Introductionmentioning

confidence: 99%

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Chen¹,

Jin²,

Qi³

et al. 2017

Biomed. Opt. Express

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Abstract:Zebrafish play an important role in biology, pharmacology, toxicology, and medicine. The cardio-cerebrovascular development of zebrafish is particularly critical to understand both brain disorders and cardiovascular diseases in human. In this paper, we applied optical resolution photoacoustic microscopy (ORPAM) to image the whole-body vasculature of the embryonic zebrafish with a special focus on the development of the cardiocerebrovascular system. Using the intrinsic optical absorption contrast of the embryo, we successfully visualized the formation of the cardio-cerebrovascular network in highresolution using a 10 × objective, and monitored the whole-body vascular development using a 4 × objective. In addition, we evaluated the impact of the eggshell and pigment inhibitor on the image quality. Our results suggest that ORPAM is capable of studying the cardiocerebrovascular development of zebrafish in the embryonic stage, and thus has the potential to investigate the cardiovascular and cerebrovascular diseases of human in the future.

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“…The axial resolution of the system is approximately 92 m determined by the center frequency of the transducer. 27 The maximum penetration depth for human hair is measured to be more than 15 mm. As shown in Figs. 2(e) and 2(f), both photoacoustic and ultrasound signals have the best signal-to-noise ratios (SNRs) of 32 dB and 34 dB at the acoustic focus, and both SNRs deteriorate when the targets locate o® the focus.…”

Section: Resultsmentioning

confidence: 99%

Co-registered photoacoustic and ultrasound imaging for tongue cancer detection

Guo

et al. 2018

J. Innov. Opt. Health Sci.

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Tongue cancer is an increasingly common disease with high morbidity. Besides clinical observation, biomedical imaging techniques have been investigated for early detection of tongue cancer. In this paper, we proposed a co-registered dual-modality photoacoustic (PA) and ultrasound imaging technique to simultaneously map the functional and structural information of human tongue, which has the potential to detect and diagnose tongue cancer in early stage. The imaging probe comprises a 20-MHz side-view focused transducer for ultrasound imaging and PA detection, a light path constructed by a multimode optical¯ber, and a prism for PA illumination. Phantom experiments were conducted to evaluate the performance of the system including penetration depth, spatial resolution and signal-to-noise ratio. In vivo imaging of animal tumor and human tongue was carried out to show the feasibility of the proposed technique to detect tumor lesions in human tongue. The results of phantom and in vivo experiments suggest that the proposed technique has the potential to detect the early-stage cancer lesions in human tongue.

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Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle

Cited by 13 publications

References 31 publications

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Co-registered photoacoustic and ultrasound imaging for tongue cancer detection

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