In vivo study of rat cortical hemodynamics using a stereotaxic‐apparatus‐compatible photoacoustic microscope

Abstact Photoacoustic microscopy (PAM) provides a new method for the imaging of small‐animals with high‐contrast and deep‐penetration. However, the established PAM systems have suffered from a limited field‐of‐view or imaging speed, which are difficult to both monitor wide‐field activity of organ and record real‐time change of local tissue. Here, we reported a dual‐raster‐scanned photoacoustic microscope (DRS‐PAM) that integrates a two‐dimensional motorized translation stage for large field‐of‐view imaging and a two‐axis fast galvanometer scanner for real‐time imaging. The DRS‐PAM provides a flexible transition from wide‐field monitoring the vasculature of organs to real‐time imaging of local dynamics. To test the performance of DRS‐PAM, clear characterization of angiogenesis and functional detail was illustrated, hemodynamic activities of vasculature in cerebral cortex of a mouse were investigated. Furthermore, response of tumor to treatment were successfully monitored during treatment. The experimental results demonstrate the DRS‐PAM holds the great potential for biomedical research of basic biology.

Section: Resultsmentioning

confidence: 99%

Wide‐field monitoring and real‐time local recording of microvascular networks on small animals with a dual‐raster‐scanned photoacoustic microscope

Yang

Wang

Zhang

et al. 2020

“…[8][9][10] Photoacoustic imaging, an emerging imaging technology, has been proven to specifically image the lipid component as well as the structure information. [11][12][13][14][15][16] So far, there is no related study on this topic yet for IVPA imaging validation.…”

Section: Introductionmentioning

confidence: 99%

Reliability assessment on intravascular photoacoustic imaging of lipid: ex vivo animal and human sample validation

Peng

Hao

Wang

et al. 2020

Although the lipid-detecting IVPA imaging system has been developed in resolution, speed, and catheter size, there is no parameterization study of the reliability on the IVPA imaging for lipid diagnosis. Here, the sensitivity, specificity, and accuracy were calculated to assess the reliability of the IVPA imaging of lipid. Abdominal aortas from six rabbits with atherosclerosis, were subjected to the IVPA imaging and Oil Red O staining, and 75 groups of IVPA as well as corresponding histological images were obtained. Similarly, 125 groups of IVPA and histological results were obtained from five human carotid plaque samples. The sensitivity, specificity, and accuracy, calculated from the statistical data, were 96.8%, 83.3%, 94.6% and 97.3%, 72.7%, 95.2%, respectively. The numerical values of sensitivity, specificity, and accuracy demonstrated the reliability of IVPA imaging on distinguishing the lesions vessel with lipid-rich plaque, which provided the foundation for IVPA translation to clinical application.

“…However, this technique is invasive and only able to visualize hemodynamics in one spatial point. Utilization of an electrical‐micro‐mechanical‐system (MEMS) based optical scanner, we reported an ultracompact photoacoustic microscope with a size of 22 × 30 × 13 mm 3 and a weight of 20 g, which was still not appropriate for freely moving rodents .…”

Section: Introductionmentioning

confidence: 99%

Wearable optical resolution photoacoustic microscopy

Chen

Xie

2019

Self Cite

Optical resolution photoacoustic microscopy (ORPAM) is an emerging imaging technique, which has been extensively used to study various brain activities and disorders of the anesthetized/restricted rodents with a special focus on the morphological and functional visualization of cerebral cortex. However, it is challenging to develop a wearable photoacoustic microscope, which enables the investigation of brain activities/disorders on freely moving rodents. Here, we report a wearable and robust optical resolution photoacoustic microscope (W-ORPAM), which utilizes a small, light, stable and fast optical scanner. This wearable imaging probe features high spatiotemporal resolution, large field of view (FOV) and easy assembly as well as adjustable optical focus during the in vivo experiment, which makes it accessible to image cerebral cortex activities of freely moving rodents. To demonstrate the advantages of this technique, we used W-ORPAM to monitor both morphological and functional variations of vasculature in cerebral cortex during the induction of ischemia and reperfusion of a freely moving rat.