Label-Free Multi-Microfluidic Immunoassays with Liquid Crystals on Polydimethylsiloxane Biosensing Chips

Fan, Yuanyuan; Chen, Fu Lun; Liou, Jian Chiun; Huang, Yu; Chen, Chun Han; Hong, Zi Yin; Lin, James T.; Hsiao, Yu Cheng

doi:10.3390/polym12020395

Cited by 28 publications

(21 citation statements)

References 33 publications

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“…Liquid crystal is a birefringent material that is powerful enough for the modulation of polarization. With the features of controllable birefringence and refractive indices, liquid crystal has been applied in various fields, such as displays [5], ophthalmic optics [6][7][8], beam shaping [9][10][11], and bio-sensors [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Diffraction and Polarization Properties of Electrically–Tunable Nematic Liquid Crystal Grating

Huang

Kang

et al. 2020

Polymers

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This work demonstrates an electrically-tunable nematic liquid crystal (NLC) diffraction grating with a periodic electrode structure, and discusses the polarization properties of its diffraction. The efficiency of the first-order diffraction can be gradually controlled by applying external electric fields cross the NLC, and the maximum diffraction efficiency of the first-order diffraction that can be obtained is around 12.5% under the applied voltage of 5.0 V. In addition to the applied electric field, the efficiency of the first-order diffraction can also vary by changing the polarized state of the incident beam. Antisymmetric polarization states with symmetrical intensities in the diffractions corresponding to the +1 and −1 order diffraction signals are also demonstrated.

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Section: Introductionmentioning

confidence: 99%

Diffraction and Polarization Properties of Electrically–Tunable Nematic Liquid Crystal Grating

Huang

Kang

et al. 2020

Polymers

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“…Once rinsed with DI water to remove unbound BSA, the cells of each DTSG were self-assembled with a 10 μm spacer. The details of the BSA solution immobilization and device fabrication can be found in past publications [ 17 , 18 ]. The DFCLC changed to the P state with increasing concentrations of BSA.…”

Section: Resultsmentioning

confidence: 99%

“…The DFCLC changed to the P state with increasing concentrations of BSA. Ultimately, the qui-perfect P state led the DTSG biosensors to be in a reflective perfect P state ( Figure 5 ) [ 17 ]. In addition, the DHO effect produced by the electrical features of DTSG can be applied to bioinspired sensing with regard to antibacterial surfaces.…”

Section: Resultsmentioning

confidence: 99%

Dielectric Thermal Smart Glass Based on Tunable Helical Polymer-Based Superstructure for Biosensor with Antibacterial Property

et al. 2021

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A dielectric thermal smart glass (DTSG) based on the dielectric heating optical (DHO) effect in tunable helical polymer-based superstructures—cholesteric liquid crystals (CLCs)—was exhibited in this study. Field-induced dielectric heating can strongly affect the orientation of liquid crystals and change its optical properties. The purpose of this research focuses on dual-frequency CLC materials characterized by their specific properties on dielectric relaxation and demonstrates their potential for antibacterial biosensor applications. The developed DTSG is driven by voltages with modulated frequencies. The principal of DTSG in transparent states are a planar (P) state and a heated planar (HP) state reflecting infrared light, operated with the voltage at low and high frequencies, respectively. The scattering states are a focal conic (FC) state and a heated FC (HFC) state, with an applied frequency near the crossover frequency. The biomolecule detection of the antibacterial property was also demonstrated. The detection limitation of the DTSG biosensor was found to be about 0.5 µg/mL. The DTSG material has many potential industrial applications, such as in buildings, photonic devices, and biosensor applications.

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“…Compared to existing label-free techniques, the color-indicating LCs bioinspired sensor can be used as a microfluidic immunoassay application. 20 …”

Section: Discussionmentioning

confidence: 99%

Label-Free, Smartphone-Based, and Sensitive Nano-Structural Liquid Crystal Aligned by Ceramic Silicon Compound–Constructed DMOAP-Based Biosensor for the Detection of Urine Albumin

Chuang¹,

Lin²,

Wang³

et al. 2021

IJN

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Introduction The sensitive interfacial interaction of liquid crystals (LC) holds potential for precision biosensors. In the past, the developments of LC biosensors were limited by the complicated manufacturing process, which hinders commercialization and wider applications of such devices. In this report, we demonstrate the first nano-structural polymeric stabilized-cholesteric LC (PSCLC) thin films to be a new label-free biosensing technology. Methods The transmission spectra of PSCLC devices were measured by the fiber-optic spectrometer with high-resolution. In addition, a smartphone was set on the stage, and the camera of smartphone was placed and aligned with a set of lenses embedded in the designed stage. To decrease the chromatic and spherical aberrations, an achromatic lens set composition, consisting of both dual-convex lens and concave-plane lens, was applied for measuring and imaging the PSCLC texture. The average and the estimated standard deviation (SD) were used to present quantitative experimental results. The test BSA was immobilized and fulfilled by the ceramic silicon-constructed DMOAP-coated glass in aqueous BSA solutions at 1 mg/mL, 1 µg/mL, and 1 ng/mL. Results The fabrication process of PSCLC is much simplified compared to previous LC biosensors. The color of PSCLC biosensor altered with the BSA concentration, making detection result easy to read. The detection limit of 1 ng/mL is achieved for label-free PSCLC biosensor. The PSCLC biosensor was able to successfully detect due to the albumin concentration’s alteration, with a linear range of 1 ng/mL–2 mg/mL. Thus, the label-free-proposed design-integrated nanoscale PSCLCs smartphone-based biosensor could successfully detect BSA in a preclinical urine sample. Conclusion Finally, we propose a design to integrate the PSCLC biosensor with a smartphone. The PSCLC owns potential for high performance, low cost for detecting various disease biomarkers in home use. Owing to its great potential for high performance and low cost, the PSCLC biosensors can be used as a label-free point-of-care for detecting various disease biomarkers for patients in care homes.

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Label-Free Multi-Microfluidic Immunoassays with Liquid Crystals on Polydimethylsiloxane Biosensing Chips

Cited by 28 publications

References 33 publications

Diffraction and Polarization Properties of Electrically–Tunable Nematic Liquid Crystal Grating

Diffraction and Polarization Properties of Electrically–Tunable Nematic Liquid Crystal Grating

Dielectric Thermal Smart Glass Based on Tunable Helical Polymer-Based Superstructure for Biosensor with Antibacterial Property

Label-Free, Smartphone-Based, and Sensitive Nano-Structural Liquid Crystal Aligned by Ceramic Silicon Compound–Constructed DMOAP-Based Biosensor for the Detection of Urine Albumin

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