Label-Free Monitoring of Single Molecule Immunoreaction with a Nanopipette

Yu, Ru‐Jia; Ying, Yi‐Lun; Hu, Yong‐Xu; Gao, Rui

doi:10.1021/acs.analchem.7b01921

Cited by 54 publications

(50 citation statements)

References 31 publications

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“…The successful assembly of DNA origami folding from an M13mp18 ssDNA scaffold following previous reports [16,18] was confirmed by atomic force microscopy (AFM). Figure 2 shows representative examples of all four assembled DNA origami structures.…”

Section: Resultssupporting

confidence: 67%

“…[15] An alternative strategy for single-molecule sensing employed by Edel et al involved protein detection using nucleic aptamer-modified DNA carriers [16] and DNA aptamer-functionalized gold nanoparticles. [18] This body of work illustrates that DNA conjugates and carriers, nanoparticles and aptamers can increase the sensitivity and signal for selective detection of molecules using nanopipettes. [18] This body of work illustrates that DNA conjugates and carriers, nanoparticles and aptamers can increase the sensitivity and signal for selective detection of molecules using nanopipettes.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of 2D DNA Origami with Nanopipettes

et al. 2018

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Nanopipettes are a useful tool for the detection and analysis of biological molecules at the single-molecule level. Here, we employ nanopipettes fabricated from glass capillaries to identify differently structured 2D DNA origami through the distinctive amplitude and dwell time of the ion current peak resulting from the translocation of the origami through the nanopipette pore. We demonstrate that the ion current peak originating from frame-like DNA origami comprises two individual peaks in contrast to solid tiles of similar size that only lead to a single peak in the ion current. Interestingly, the fine details of the shape of the double-peak characteristic of the translocation of DNA origami frames are correlated with the structural features of the DNA origami. In particular, the size of the central cavity governs the lag time between the two constituent peaks of the double-peak. This work demonstrates the ability of glass nanopipettes to identify and characterize differently structured DNA origami of similar size, advancing the potential of nanopipettes for high-sensitivity single-molecule studies.

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Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Analysis of 2D DNA Origami with Nanopipettes

et al. 2018

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“…According to the latest report 11 , the sequencing of a human genome was attained with high accuracy using biological nanopore DNA sequencers distributed by Oxford Nanopore Technologies, Ltd. Nanopores are also used as molecular-sized Coulter counters 12 – 20 . For example, the monitoring of single-molecule immunoreactions was demonstrated by counting the number of current-blockade events derived from antigen-antibody complexes passing through a nanopore 19 . In addition, target DNA sequence detection has also been demonstrated by binding peptide nucleic acid (PNA) or PNA-polyethylene glycol (PEG) labels to the target sequences and detecting the changes in current resulting from the labelled DNA passing through a nanopore 20 .…”

Section: Introductionmentioning

confidence: 99%

Two-step breakdown of a SiN membrane for nanopore fabrication: Formation of thin portion and penetration

Yanagi

Hamamura

Akahori

et al. 2018

Sci Rep

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For the nanopore sensing of various large molecules, such as probe-labelled DNA and antigen-antibody complexes, the nanopore size has to be customized for each target molecule. The recently developed nanopore fabrication method utilizing dielectric breakdown of a membrane is simple and quite inexpensive, but it is somewhat unsuitable for the stable fabrication of a single large nanopore due to the risk of generating multiple nanopores. To overcome this bottleneck, we propose a new technique called “two-step breakdown” (TSB). In the first step of TSB, a local conductive thin portion (not a nanopore) is formed in the membrane by dielectric breakdown. In the second step, the created thin portion is penetrated by voltage pulses whose polarity is opposite to the polarity of the voltage used in the first step. By applying TSB to a 20-nm-thick SiN membrane, a single nanopore with a diameter of 21–26 nm could be fabricated with a high yield of 83%.

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“…Yu and coworkers fabricated a biomarker sensor based on a quartz nanopipette for protein detection. In their work, AFP, as the target biomarker, was detected with or without its antibody in a label-free method [ 173 ].…”

Section: Microstructure-based Biomarker Sensorsmentioning

confidence: 99%

Recent Progress of Biomarker Detection Sensors

Liu

Cui

2020

Research

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Early cancer diagnosis and treatment are crucial research fields of human health. One method that has proven efficient is biomarker detection which can provide real-time and accurate biological information for early diagnosis. This review presents several biomarker sensors based on electrochemistry, surface plasmon resonance (SPR), nanowires, other nanostructures, and, most recently, metamaterials which have also shown their mechanisms and prospects in application in recent years. Compared with previous reviews, electrochemistry-based biomarker sensors have been classified into three strategies according to their optimizing methods in this review. This makes it more convenient for researchers to find a specific fabrication method to improve the performance of their sensors. Besides that, as microfabrication technologies have improved and novel materials are explored, some novel biomarker sensors—such as nanowire-based and metamaterial-based biomarker sensors—have also been investigated and summarized in this review, which can exhibit ultrahigh resolution, sensitivity, and limit of detection (LoD) in a more complex detection environment. The purpose of this review is to understand the present by reviewing the past. Researchers can break through bottlenecks of existing biomarker sensors by reviewing previous works and finally meet the various complex detection needs for the early diagnosis of human cancer.

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Label-Free Monitoring of Single Molecule Immunoreaction with a Nanopipette

Cited by 54 publications

References 31 publications

Analysis of 2D DNA Origami with Nanopipettes

Analysis of 2D DNA Origami with Nanopipettes

Two-step breakdown of a SiN membrane for nanopore fabrication: Formation of thin portion and penetration

Recent Progress of Biomarker Detection Sensors

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