Label-free methylation specific sensor based on silicon microring resonators for detection and quantification of DNA methylation biomarkers in bladder cancer

An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections

“…To use the iROAD chip as a detection system, a previously described protocol was used with slight modifications for the detailed structure and fabrication of silicon microring resonators (SMRs) (Shin et al, 2013a(Shin et al, , 2013bPark et al, 2013). The SMR sensor device was provided from One BioMed Pte.…”

A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex

“…To design MTB-ISAD device, the modified protocol was used for the detailed structure and fabrication of silicon microring resonators (SMR) that have been previously described (Shin et al, 2013a(Shin et al, , 2013b. The device (2.5 cm  0.8 cm) structures such as microring structures, waveguides, and gratings, were patterned on a commercially available 200 mm SOI (Silicon-On-Insulator) wafer with a 220 nm thick top silicon layer and 2 μm thick buried oxide layer by 210 nm deep (ultraviolet) lithography.…”

“…The technique is based on an isothermal solid-phase amplification/detection (ISAD) assay that combines solid-phase-based isothermal DNA amplification (recombinase polymerase amplification (RPA)) and silicon microring resonator (SMR)-based biophotonic sensors, which do not involve labeling (Shin et al, 2013a). Although the SMRs have been used for direct detection of nucleic acids based on the hybridization mechanism between the capture probe and the target (Bogaerts et al, 2012;Baaske and Vollmer, 2012;Shin et al, 2013bShin et al, , 2013cIqbal et al, 2010), however, the MTB-ISAD technique is based on the amplification and the detection mechanism of nucleic acids that was increased the sensitivity of the nucleic acid detection by the combination. Using this strategy, MTB nucleic acid from human specimens is simultaneously amplified and detected by a grafted complementary primer on the SMR in a label-free and real-time manner.…”

Biomolecular analysis with microring resonators: applications in multiplexed diagnostics and interaction screening

“…Numerous label-free, microring-based assays have been developed for nucleic acids, including DNA [15-19], miRNA [20], and tmRNA [21]. Highlights of these studies include low detection limits (10 pM), single nucleotide polymorphism (SNP) discrimination [16], and analysis of DNA methylation patterns [17].…”

“…Highlights of these studies include low detection limits (10 pM), single nucleotide polymorphism (SNP) discrimination [16], and analysis of DNA methylation patterns [17]. An impressive application of the technology paired methylation specific polymerase chain reaction (MS-PCR), bisulfite modifications, and microring resonators to quantitate methylation frequency in genomic DNA from cancer cell lines [19]. …”