Biomolecular analysis with microring resonators: applications in multiplexed diagnostics and interaction screening

Kindt, Jared T.; Bailey, Ryan C.

doi:10.1016/j.cbpa.2013.06.014

Cited by 53 publications

(34 citation statements)

References 51 publications

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“…MRRs have an advantage because sensor signal yield and sensitivity are independent from the size of the MRR, which allows advanced miniaturization [10]. This makes the application of MRRs as a microarray sensor element for multiplex biosensing very feasible [11]. Recently, we have described the development and characteristics of a MRR sensor component in the TriPleX platform [12].…”

Section: Introductionmentioning

confidence: 99%

Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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Integrated-optical (bio) chemical sensors are more and more commercialized, mainly because of their high intrinsic sensitivity in combination with the possibilities they offer for integration in optofluidic devices. The microring resonator (MRR), for example, is a very feasible structure to be used as microarray sensor element for multiplex biosensing. Fabrication of MRRs has been described in the case of the TriPleX platform employing well-defined stacks of stoichiometric Si 3 N 4 and SiO 2 material. This platform allows for a wide variety of applications, due to its intrinsic low loss and its transparency for the VIS/NIR wavelength range. In the present paper, we describe the recent achievements in the characterization of arrays of these MRRs regarding temperature sensitivity, refractive index sensitivity and protein immobilization performance. Furthermore, the use of a reference channel/reference MRR is demonstrated in order to show the advantage of compensation of unintended change in temperature or sample composition. The refractive index sensitivity was determined to be 104 nm/RIU and the limit of detection was about 2 × 10 -6 RIU. MRRs appeared to behave very comparable (expressed as the coefficient of (intra-array) variation (CV)) regarding the response to temperature (CV≈0.3%) as well as refractive index (CV<0.1%). Furthermore, it is shown that protein immobilization onto the different MRRs of the same arrays can be realized in a comparable way (CV<3%). A good comparability (i.e., a low variation) in these aspects allows for the use of internal referencing in order to compensate for unintended change in temperature or refractive index and also helps to attain a high assay precision in the ultimate biosensing application. Ongoing research is focused on the integration of more functionality on chip and further miniaturization in order to allow for fabrication of complex, though affordable, sensor devices.

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Section: Introductionmentioning

confidence: 99%

Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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“…Target analytes binding to the surface of the ring resonator shift the ring resonance. Initially, one of the most attractive aspects of this system was the ability to perform a wide variety of label-free interaction analyses (Kindt and Bailey 2013), but the need for higher sensitivity for clinical diagnostics has led to the use of fluorescent tags, nanoparticles or enzymatically generated precipitates to increase the signal to noise. Bailey’s version of microring resonators is now commercially available from Genaltye (http://genalyte.com; San Diego, CA, USA) in an automated system called the Maverick.…”

Section: 0 Fast Forward To the Presentmentioning

confidence: 99%

Evanescent wave fluorescence biosensors: Advances of the last decade

Taitt

Anderson

Ligler

2016

Biosensors and Bioelectronics

117

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Biosensor development has been a highly dynamic field of research and has progressed rapidly over the past two decades. The advances have accompanied the breakthroughs in molecular biology, nanomaterial sciences, and most importantly computers and electronics. The subfield of evanescent wave fluorescence biosensors has also matured dramatically during this time. Fundamentally, this review builds on our earlier 2005 review. While a brief mention of seminal early work will be included, this current review will focus on new technological developments as well as technology commercialized in just the last decade. Evanescent wave biosensors have found a wide array applications ranging from clinical diagnostics to biodefense to food testing; advances in those applications and more are described herein.

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“…With its natural benefit of high sensitivity, ultra-compactness and manufacture compatibility with the standard complementary metal-oxidesemiconductor (CMOS) process [3,4], advanced SOI sensors were able to be fabricated with low cost and mature industrial infrastructure [5]. Under this category, many relevant results have been reported, including surface plasmon resonance(SPR) [6], photonic crystals [7], Mach-Zehnder interferometer [8], micro-disk [9] and microring resonator [10].…”

Section: Introductionmentioning

confidence: 98%