Label-free in vivo fiber-based optical-resolution photoacoustic microscopy

Reza, Parsin Haji; Shi, Wei; Zemp, Roger J.

doi:10.1364/ol.36.004107

Cited by 90 publications

(69 citation statements)

References 12 publications

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“…2(B)). Far-red and infra-red spectral peaks have estimated pulse energies as high as ~100 ± 50nJ, higher than pulse energies used in previous OR-PAM experiments [11,13]. Four-wave mixing may be one source of SRS peak broadening as two or more wavelength may interact in order to produce an output at various sum or difference frequencies [23].…”

Section: Methodsmentioning

confidence: 95%

“…Furthermore, OR-PAM has proven useful for quantifying functional parameters down to capillary sizes [8]. Recent OR-PAM developments include real-time systems [9,10], small handheld systems [11,12], endoscopy systems [13][14][15], and novel detection schemes [16,17]. Fiber and microchip lasers have recently been introduced as high-repetition-rate sources for realtime OR-PAM [9,18]; however, the wavelength tunability was limited.…”

Section: Introductionmentioning

confidence: 99%

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In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

Reza

Forbrich

Zemp

2014

Biomed. Opt. Express

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Abstract:In this paper a multi-wavelength optical-resolution photoacoustic microscopy (OR-PAM) system using stimulated Raman scattering is demonstrated for both phantom and in vivo imaging. A 1-ns pulse width ytterbium-doped fiber laser is coupled into a single-mode polarization maintaining fiber. Discrete Raman-shifted wavelength peaks extending to nearly 800 nm are generated with pulse energies sufficient for OR-PAM imaging. Bandpass filters are used to select imaging wavelengths. A dualmirror galvanometer system was used to scan the focused outputs across samples of carbon fiber networks, 200μm dye-filled tubes, and Swiss Webster mouse ears. Photoacoustic signals were collected in transmission mode and used to create maximum amplitude projection C-scan images. Double dye experiments and in vivo oxygen saturation estimation confirmed functional imaging potential.

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Section: Methodsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

Reza

Forbrich

Zemp

2014

Biomed. Opt. Express

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“…OR-PAM images are obtained by scanning a diffraction limited focused spot which can be done deep in tissues using a fiber bundle optical endoscope [11,16] and detecting the sound with an external US transducer. Fully encapsulated AR-PAM endoscopes have been demonstrated by Yang et al [17,18] using a single mode fiber and an actuation system for the beam steering.…”

Section: Introductionmentioning

confidence: 99%

Towards new applications using capillary waveguides

Stasio¹,

Shibukawa²,

Papadopoulos³

et al. 2015

Biomed. Opt. Express

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Abstract:In this paper we demonstrate the enhancement of the sensing capabilities of glass capillaries. We exploit their properties as optical and acoustic waveguides to transform them potentially into high resolution minimally invasive endoscopic devices. We show two possible applications of silica capillary waveguides demonstrating fluorescence and opticalresolution photoacoustic imaging using a single 330 μm-thick silica capillary. A nanosecond pulsed laser is focused and scanned in front of a capillary by digital phase conjugation through the silica annular ring of the capillary, used as an optical waveguide. We demonstrate optical-resolution photoacoustic images of a 30 μm-thick nylon thread using the water-filled core of the same capillary as an acoustic waveguide, resulting in a fully passive endoscopic device. Moreover, fluorescence images of 1.5 μm beads are obtained collecting the fluorescence signal through the optical waveguide. This kind of silica-capillary waveguide together with wavefront shaping techniques such as digital phase conjugation, paves the way to minimally invasive multi-modal endoscopy.

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“…Both images are formed using a 3 mm × 3 mm FPI. The small footprint and high sensitivity of the FPI makes this system a promising method for OR-PAM handheld and endoscopy applications [14,15].…”

mentioning

confidence: 99%

In vivo optical resolution photoacoustic microscopy using glancing angle-deposited nanostructured Fabry–Perot etalons

et al. 2015

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=f49f0e62-7df9-4107-9daf-e1005866b26f http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=f49f0e62-7df9-4107-9daf-e1005866b26fIn vivo optical resolution photoacoustic microscopy using glancing angle-deposited nanostructured Fabry-Perot etalons In this Letter, reflection-mode optical resolution photoacoustic microscopy (OR-PAM) using glancing angledeposited (GLAD) nanostructured Fabry-Perot interferometers (FPI) for in vivo applications is reported. GLAD is a single-step physical vapor deposition (PVD) technique used to fabricate porous nanostructured thin films. Using titanium dioxide, a transparent semiconductor with a high refractive index (n 2.4), the GLAD technique can be employed to fabricate samples with tailored nano-porosity, refractive index periodicities, and high Q-factor reflectance spectra. The OR-PAM in vivo images of chorioallantoic membrane (CAM) of 5-day chicken embryo model are demonstrated. The phantom study shows lateral resolution and signal-to-noise ratio better than 7 μm and 35 dB, respectively. The sensitive GLAD FPI allows photoacoustic imaging down to a few-nJ pulse energy. To the best of our knowledge, this is the first time that a FPI-based reflection mode optical resolution photoacoustic imaging technique is demonstrated for in vivo applications.

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Label-free in vivo fiber-based optical-resolution photoacoustic microscopy

Cited by 90 publications

References 12 publications

In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

Towards new applications using capillary waveguides

In vivo optical resolution photoacoustic microscopy using glancing angle-deposited nanostructured Fabry–Perot etalons

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