Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy

Gao, Liang; Zhou, Haijun; Thrall, Michael J.; Li, Fuhai; Yang, Yue; Wang, Zhiyong; Luo, Pengfei; Wong, Kelvin; Palapattu, Ganesh S.; Wong, Stephen T.C.

doi:10.1364/boe.2.000915

Cited by 68 publications

(57 citation statements)

References 27 publications

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“…This limits the scalability in terms of cell population size, resolution, and molecular complexity. Although nonlinear Raman techniques such as CARS and SRS allow faster imaging, full-spectrum acquisition and interpretation are not as straightforward [7,8].…”

Section: Introductionmentioning

confidence: 99%

High-speed hyperspectral Raman imaging for label-free compositional microanalysis

Shih

2013

Biomed. Opt. Express

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Abstract:We present high-speed hyperspectral Raman imaging with integrated active-illumination for label-free compositional microanalysis. We show that high-quality Raman spectra can be acquired from as many as ~1,000 spots/sec semi-randomly distributed among a ~100x100 μm 2 area without mechanical scanning. We demonstrate rapid data acquisition from three types of samples: 1) uniform, strong Raman scatterers, e.g., silicon substrates; 2) non-uniform, medium-strength Raman scatterers, e.g., polymer microparticles; and, 3) non-uniform, relatively weak Raman scatterers, e.g., bacterial spores. We compare the system performance to that of point-scan with an electron-multiplied CCD camera, as implemented in some commercial systems. The results suggest that our system not only provides significant imaging speed advantage for various types of samples, but also permits substantially longer integration time per spot, leading to superior signal-to-noise ratio data. Our system enables the rapid collection of high quality Raman spectra for reliable and robust compositional microanalysis that are potentially transformative in applications such as semiconductor material and device, polymer blend and biomedicine.

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Section: Introductionmentioning

confidence: 99%

High-speed hyperspectral Raman imaging for label-free compositional microanalysis

Shih

2013

Biomed. Opt. Express

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“…The methods have been validated by comparison with H & E stained images [41,42]. Similar approaches have been applied to analyze CARS images from breast cancer [43], lung carcinoma [44] and prostate cancer [45].…”

Section: Methodsmentioning

confidence: 99%

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Meyer

Schmitt

Dietzek

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSMultimodal nonlinear microscopy has matured during the past decades to one of the key imaging modalities in life science and biomedicine due to its unique capabilities of label-free visualization of tissue structure and chemical composition, high depth penetration, intrinsic 3D sectioning, diffraction limited resolution and low phototoxicity. This review briefly summarizes first recent advances in the field regarding the methodology, e.g., contrast mechanisms and signal characteristics used for contrast generation as well as novel image processing approaches. The second part deals with technologic developments emphasizing improvements in penetration depth, imaging speed, spatial resolution and nonlinear labeling strategies. The third part focuses on recent applications in life science fundamental research and biomedical diagnostics as well as future clinical applications.Multimodal nonlinear microscopy of tissue.

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“…Our ongoing work includes combination of previous and current SHG and CARS imaging platform and endo-microscopy, which is used in place of the upright, microscope for tissue imaging in real time on-site cancer diagnosis. We expect that the label-free multimodal nonlinear endo-microscope can potentially be applied to video-rate in vivo tissue imaging during intraoperative or interventional procedures and save precious time in sending tissue specimens for sectioning, staining, and diagnosis in a pathology laboratory [42][43][44].…”

Section: Multi-modal Prostate Cancer Tissue Imagingmentioning

confidence: 99%

Multimodal nonlinear endo-microscopy probe design for high resolution, label-free intraoperative imaging

McCormick³

et al. 2015

Biomed. Opt. Express

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We present a portable, multimodal, nonlinear endo-microscopy probe designed for intraoperative oncological imaging. Application of a four-wave mixing noise suppression scheme using dual wavelength wave plates (DWW) and a polarization-maintaining fiber improves tissue signal collection efficiency, allowing for miniaturization. The probe, with a small 14 mm transversal diameter, includes a customized miniaturized two-axis MEMS (micro-electromechanical system) raster scanning mirror and microoptics with an illumination laser delivered by a polarization-maintaining fiber. The probe can potentially be integrated into the arms of a surgical robot, such as da Vinci robotic surgery system, due to its minimal cross sectional area. It has the ability to incorporate multiple imaging modalities including CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), and TPEF (two-photon excited fluorescence) in order to allow the surgeon to locate tumor cells within the context of normal stromal tissue. The resolution of the endo-microscope is experimentally determined to be 0.78 µm, a high level of accuracy for such a compact probe setup. The expected resolution of the as-built multimodal, nonlinear, endo-microscopy probe is 1 µm based on the calculation tolerance allocation using Monte-Carlo simulation. The reported probe is intended for use in laparoscopic or radical prostatectomy, including detection of tumor margins and avoidance of nerve impairment during surgery. "Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy," Biomed. Opt. Express 2(4), 915-926 (2011). 44. X. Xu, J. Cheng, M. J. Thrall, Z. Liu, X. Wang, and S. T. C. Wong, "Multimodal non-linear optical imaging for label-free differentiation of lung cancerous lesions from normal and desmoplastic tissues," Biomed. Opt. Express 4(12), 2855-2868 (2013).

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Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy

Cited by 68 publications

References 27 publications

High-speed hyperspectral Raman imaging for label-free compositional microanalysis

High-speed hyperspectral Raman imaging for label-free compositional microanalysis

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Multimodal nonlinear endo-microscopy probe design for high resolution, label-free intraoperative imaging

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