Label-free fluorescent assay for high sensitivity and selectivity detection of acid phosphatase and inhibitor screening

Wang, Jing; Yan, Yu; Yan, Xu; Hu, Tianyu; Tang, Xiaojian; Su, Xingguang

doi:10.1016/j.snb.2016.05.024

Cited by 31 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To this end, some fluorescent chemosensors based on zinc complexes have been designed and used for ATP detection under biological pH conditions. − However, the single recognition site in these fluorescent probes limits their selectivity to ATP compared with that of other phosphate anions. Taking advantage of the specific recognition, some aptamer-based fluorescence sensing assays have been developed for the selective detection of ATP. − Nevertheless, the design and synthesization of a fluorescent probe for ATP sensing with high sensitivity and selectivity is still urgently required.…”

Section: Introductionmentioning

confidence: 99%

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Cui

Yang

Yao

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Water-soluble conjugated polymers are attractive fluorescent materials for applications in chemical and biological sensing. The molecular wire effect of such polymers amplifies changes in the fluorescence signal, which can be used for detecting various analytes with high sensitivity. In this work, we report an efficient ratiometric fluorescent probe based on a water-soluble conjugated polymer that showed high sensitivity and selectivity toward adenosine 5'-triphosphate (ATP). The macromolecular probe consisted of a polyfluorene backbone doped with 5 mol % 1,4-dithienylbenzothiadiazole (DBT) modified by bis-imidazolium and oligo(ethylene glycol) moieties. Solutions of the polymer emitted purple fluorescence, which changed to red upon addition of ATP molecules. The addition of ATP caused the polymer to aggregate, which enhanced fluorescence resonance energy transfer efficiency from the fluorene segments to DBT units, leading to an increase in red emission. The ratio of the fluorescence at these different wavelengths (I/I) showed a strong dependence on the ATP concentration. PF-DBT-BIMEG also exhibited high selectivity for ATP sensing over other representative anions and discriminated it from adenosine 5'-diphosphate (ADP) and adenosine 5'-monophosphate (AMP). This can be explained by the much stronger electrostatic interactions between the polymer and ATP than the interactions between the polymer and ADP or AMP, as confirmed through molecular dynamics simulations.

show abstract

Section: Introductionmentioning

confidence: 99%

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Cui

Yang

Yao

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Upon the preincubation of OPs with AChE, ATCh cannot be hydrolyzed into thiocholine because of the depressed AChE activity. Further, ATCh only has the thioether bond and quaternary ammonium salt group and consequently cannot wrest the CdS QDs from CdS/PPT/ITO via the formation of stronger chemical bonds. − In this context, PEC current would not be decreased.…”

Section: Resultsmentioning

confidence: 99%

Electropolymerization-Induced Positively Charged Phenothiazine Polymer Photoelectrode for Highly Sensitive Photoelectrochemical Biosensing

Wang

et al. 2019

Anal. Chem.

View full text Add to dashboard Cite

Exploring the fabrication of an electrode with high photoelectric conversion efficiency and abundant functional groups for ideal photoelectrochemical (PEC) sensor development is highly urgent but faces a significant challenge. Herein we report an electropolymerization strategy for the preparation of phenothiazine polymeric film on an indium tin oxide (ITO) surface (PPT/ITO), within only a few seconds, and monomers. The fabricated PPT/ITO electrode possessed excellent stability and abundant quaternary ammonium salt groups for developing a highly sensitive PEC sensor through electrostatic binding with negatively charged materials. In this context, a CdS QDs-functionalized PPT/ITO electrode (CdS/PPT/ITO) was proposed and applied to the analysis of chlorpyrifos, used as a model target organophosphorous pesticide (OP). The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. As compared to the case where chlorpyrifos is absent, significantly enhanced PEC current is determined and is proportional to chlorpyrifos amounts. Thus, the developed CdS/PPT/ITO-based PEC sensor achieved excellent chlorpyrifos biosensing with improved sensitivity down to approximately ng/mL level with good specificity. We envision the proposed strategy will provide a new path to conveniently fabricate photoelectrodes possessing high performance, which will have more useful applications in PEC sensing.

show abstract

“…Through quantifying alcohols generated from ACP-catalyzed dephosphorylation of various orthophosphoric monoesters, several methods have been developed to probe ACP activity, including electrochemistry, , colorimetry, − fluorimetry, − potentiometric immunoassay, and chromatography . Among these methods, colorimetry, featuring simplicity, cost-effectiveness, rapidity, and visibility, has attracted extensive attention.…”

Section: Introductionmentioning

confidence: 99%

Engineering Two-Dimensional Pd Nanoplates with Exposed Highly Active {100} Facets Toward Colorimetric Acid Phosphatase Detection

Chen

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Enzyme-like activity and efficiency of nanomaterials are strongly controlled by their size, composition, and structure, and hence the structural parameters need to be optimized. Here, we report that two-dimensional Pd nanoplates enclosed by {100}-facets [{100}PdSP@rGO] exhibit substantially enhanced intrinsic oxidase-like activities relative to the {111}-facets ones and Pd nanocubes in catalyzing the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine. By taking ascorbic acid 2-phosphate as the substrate, which transforms to ascorbic acid in the presence of acid phosphatase (ACP), the {100}PdSP@rGO could be used as an efficient nanozyme for colorimetric ACP detection without resorting to destructive H2O2. A good linear relationship from 0.01 to 6.0 mU/mL with a detection limit of 8.3 μU/mL is obtained, which is better than most previously reported ACP assays. Importantly, the excellent assay performance could be successfully applied to ACP determination in serum samples with high accuracy. This study demonstrates that the enzyme-like activity of nanomaterials could be finely tuned simultaneously by controlling their exposed crystal facets and high specific surface area.

show abstract

Label-free fluorescent assay for high sensitivity and selectivity detection of acid phosphatase and inhibitor screening

Cited by 31 publications

References 35 publications

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Electropolymerization-Induced Positively Charged Phenothiazine Polymer Photoelectrode for Highly Sensitive Photoelectrochemical Biosensing

Engineering Two-Dimensional Pd Nanoplates with Exposed Highly Active {100} Facets Toward Colorimetric Acid Phosphatase Detection

Contact Info

Product

Resources

About