Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor

Nguyen, Binh Hai; Tran, Lam Dai; Quan, Do Phuc; Nguyen, Huy H.; Tran, Ngoc Huan; Nguyen, Phuc

doi:10.1016/j.msec.2013.01.044

Cited by 140 publications

(78 citation statements)

References 41 publications

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“…Recently, aptamer sequences possessing a high affinity for AFs (different structural analogs) have been reported in either publications or patents (Table 3) [66][67][68][69][70]. In the present scenario, the problems associated with the analysis of complex samples, such as blood, serum, and cellular extracts, have been solved using electrochemical biosensors [71,72].…”

Section: Aptamer-based Sensing Strategies For Aflatoxins (Afs) Detectionmentioning

confidence: 99%

“…The various developed electrochemical aptasensors for AFs detection has been summarized in Table 4. Nguyen et al have reported the label-free electrochemical aptasensor for detection of AFM1 [69]. For the construction of electrochemical aptasensor, a Fe 3 O 4 polyaniline (Fe 3 O 4 /PANi) film was polymerized on the interdigitated electrode (IDE) for AFM1 detection.…”

Section: Electrochemical Aptasensors For Afs Detectionmentioning

confidence: 99%

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Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

et al. 2016

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Abstract:Mycotoxin are small (MW~700 Da), toxic secondary metabolites produced by fungal species that readily colonize crops and contaminate them at both pre-and post-harvesting. Among all, aflatoxins (AFs) are mycotoxins of major significance due to their presence in common food commodities and the potential threat to human health worldwide. Based on the severity of illness and increased incidences of AFs poisoning, a broad range of conventional and analytical detection techniques that could be useful and practical have already been reported. However, due to the variety of structural analogous of these toxins, it is impossible to use one common technique for their analysis. Numerous recent research efforts have been directed to explore alternative detection technologies. Recently, immunosensors and aptasensors have gained promising potential in the area of sample preparation and detection systems. These sensors offer the advantages of disposability, portability, miniaturization, and on-site analysis. In a typical design of an aptasensor, an aptamer (ssDNA or RNA) is used as a bio-recognition element either integrated within or in intimate association with the transducer surface. This review paper is focused on the recent advances in electrochemical immunoand aptasensing platforms for detection of AFs in real samples.

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Section: Aptamer-based Sensing Strategies For Aflatoxins (Afs) Detectionmentioning

confidence: 99%

Section: Electrochemical Aptasensors For Afs Detectionmentioning

confidence: 99%

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

et al. 2016

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“…Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus [1][2][3]. These poisons can be found in grain seeds, linen, nuts, and feedstuff of ambush, if cows pasturage from infected food, aflatoxin B 1 (AFB 1 ) hydroxylase by the hepatic enzyme of cytochrome P450 and changed to aflatoxin M 1 (AFM 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, it is considered as an element that causes mutant and also as a carcinogenic factor. The existence of the small amount of AFM 1 for a long period of time in edible milk is important and causes liver cancer, Rey syndrome, chronic hepatitis, DNA trauma, and chromosome aberration [1]. Since milk is the main food of suckling, this group is more in danger of cancer and the diseases that are caused by these poisons.…”

Section: Introductionmentioning

confidence: 99%

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Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

et al. 2017

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Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus. The studies on different parts of Iran showed that AFM 1 infection of milk is higher than European Union's standard. In this study, the specific aptamer with the carboxylic group at the end of 5′ and Fe 3 O 4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe 3 O 4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM 1 was investigated in adjacent of AFM 1 and aflatoxin B 1 . Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe 3 O 4 -APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM 1 was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM 1 and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. Graphical abstractKeywords Aflatoxin · Aptamer · Fe 3 O 4 nanoparticle · Detoxification · Mycotoxin

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Biosensors for Pesticide Detection

Tran

2023

Biosensors Nanotechnology

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Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor

Cited by 140 publications

References 41 publications

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Biosensors for Pesticide Detection

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