Label‐Free Colorimetric Detection of Small Molecules Utilizing DNA Oligonucleotides and Silver Nanoparticles

Xu, Xiaowen; Wang, Juan; Yang, Fan; Jiao, Kui; Yang, Xiurong

doi:10.1002/smll.200901164

Cited by 70 publications

(49 citation statements)

References 38 publications

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“…The detection limit of this method was estimated to be 16 nM according to the 3σ rule, which is better or comparable to previously reported methods. [28][29][30][31][32][33] The excellent sensitivity mainly relies on the ingenious design of probes HP and P1 as well as the application of the split G-quadruplex DNAzyme. Besides the high sensitivity, the developed strategy also shows good reproducibility due to its homogeneous assay format with simple operations.…”

Section: Sensitivity Of the Coralyne Assaymentioning

confidence: 99%

“…27 Recently, some novel approaches for studying the interaction between coralyne and poly(dA) have emerged, but only a few strategies have been developed for the quantitative detection of coralyne. [28][29][30][31][32][33] Wang et al developed, respectively, AuNP-based colorimetry to quantify coralyne; however, the aggregation of metal nanoparticles is difficult to be controlled due to ubiquitous disturbances, such as the variation of the salt ion concentration, temperature, pH value and other experimental parameters, which could increase the complexity of operation and have an adverse effect on the reproducibility of their methods. Yang et al reported a quantitative assay of coralyne based on dual polarization interferometry (DPI) that depends on the mass, thickness, and refractive index (RI) changes in the surface layer of a silicon oxynitride chip, but the experimental process is complicated and requires professional staff.…”

Section: Introductionmentioning

confidence: 99%

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A Simple, Sensitive Colorimetric Assay for Coralyne Based on Target Induced Split G-quadruplex Formation

Kan

Jiang

et al. 2014

ANAL. SCI.

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The screening of potential drugs specifically binding to polydeoxyadenosine (poly(dA)) has been of great interest in recent studies. We have developed a simple colorimetric strategy through the mechanism of target induced split G-quadruplex formation for detecting coralyne, a poly(dA)-binding drug with noticeable antitumor activity. Two DNA oligonucleotides containing a split G-quadruplex sequence and an adenine-rich sequence are used in our strategy. In the presence of coralyne, the adenine-rich sequences of two oligonucleotides are drawn into close proximity, resulting in the formation of a split G-quadruplex DNAzyme that catalyzes the generation of a colored product. The DNAzyme-based colorimetric assay for coralyne has a linear range of from 0.033 to 1.667 μM with a low detection limit of 16 nM. The developed method is simple, cost-effective and visible; it holds great potential for applications in drug screening.

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Section: Sensitivity Of the Coralyne Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Simple, Sensitive Colorimetric Assay for Coralyne Based on Target Induced Split G-quadruplex Formation

Kan

Jiang

et al. 2014

ANAL. SCI.

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“…49−52 Poly A duplex formation in the presence of a π-system is well-known; however, formation of higher order structure with poly A in the presence of heterocyclic intercalators has not been well explored. 53,54 …”

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confidence: 99%

Unexpected Complex Formation between Coralyne and Cyclic Diadenosine Monophosphate Providing a Simple Fluorescent Turn-on Assay to Detect This Bacterial Second Messenger

et al. 2014

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Cyclic diadenosine monophosphate (c-di-AMP) has emerged as an important dinucleotide that is involved in several processes in bacteria, including cell wall remodeling (and therefore resistance to antibiotics that target bacterial cell wall). Small molecules that target c-di-AMP metabolism enzymes have the potential to be used as antibiotics. Coralyne is known to form strong complexes with polyadenine containing eight or more adenine stretches but not with short polyadenine oligonucleotides. Using a panel of techniques (UV, both steady state fluorescence and fluorescence lifetime measurements, circular dichroism (CD), NMR, and Job plots), we demonstrate that c-di-AMP, which contains only two adenine bases is an exception to this rule and that it can form complexes with coralyne, even at low micromolar concentrations. Interestingly, pApA (the linear analog of c-di-AMP that also contains two adenines) or cyclic diguanylate (c-di-GMP, another nucleotide second messenger in bacteria) did not form any complex with coralyne. Unlike polyadenine, which forms a 2:1 complex with coralyne, c-di-AMP forms a higher order complex with coralyne (≥6:1). Additionally, whereas polyadenine reduces the fluorescence of coralyne when bound, c-di-AMP enhances the fluorescence of coralyne. We use the quenching property of halides to selectively quench the fluorescence of unbound coralyne but not that of coralyne bound to c-di-AMP. Using this simple selective quenching strategy, the assay could be used to monitor the synthesis of c-di-AMP by DisA or the degradation of c-di-AMP by YybT. Apart from the practical utility of this assay for c-di-AMP research, this work also demonstrates that, when administered to cells, intercalators might not only associate with polynucleotides, such as DNA or RNA, but also could associate with cyclic dinucleotides to disrupt or modulate signal transduction processes mediated by these nucleotides.

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“…Based upon such importance, there have been a few reports for the detection of coralyne in aqueous media using unmodified DNA sequences, 7 fluorophores, 8 unmodified silver nanoparticles, 9 and unmodified or DNA-modified gold nanoparticles. 10,11 These methods are in common based upon the non Watson-Crick base pair interactions of adenineadenine (A-A), where coralyne (CR) strongly intercalates the poly-A/poly-A duplex via A-CR-A chemistry.…”

mentioning

confidence: 99%

Room-Temperature Colorimetric Detection of Coralyne Using DNA-Functionalized Nanoparticle Probes

Oh¹,

Han²,

Park³

et al. 2012

Bulletin of the Korean Chemical Society

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Label‐Free Colorimetric Detection of Small Molecules Utilizing DNA Oligonucleotides and Silver Nanoparticles

Abstract: Unmodified DNA and silver nanoparticles (AgNPs) are used to develop a colorimetric method for distinguishing ligands binding to homoadenine, based on DNA adsorption onto AgNPs (see picture). The targets of the label‐free assay could be small molecules rather than oligonucleotides.

Cited by 70 publications

References 38 publications

A Simple, Sensitive Colorimetric Assay for Coralyne Based on Target Induced Split G-quadruplex Formation

A Simple, Sensitive Colorimetric Assay for Coralyne Based on Target Induced Split G-quadruplex Formation

Unexpected Complex Formation between Coralyne and Cyclic Diadenosine Monophosphate Providing a Simple Fluorescent Turn-on Assay to Detect This Bacterial Second Messenger

Room-Temperature Colorimetric Detection of Coralyne Using DNA-Functionalized Nanoparticle Probes

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