Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns

Zandieh, Mohamad; Hosseini, Seyed Nezamedin; Vossoughi, Manouchehr; Khatami, Maryam; Abbasian, Sara; Moshaii, Ahmad

doi:10.1016/j.ab.2018.02.023

Cited by 37 publications

(21 citation statements)

References 32 publications

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“…In [49] work, their silver nanostructures were significantly stabilised by the Mercaptopropionic Acid (MPA), and Self Assembled Monolayer (SAM) biosensor detection limit for endotoxin E.coli was estimated at 340 pg./ml. We investigated biosensor selectivity in different experiments, taking endotoxin B.abortus as the second form of endotoxin contamination in our target samples (HBs-ag developed at the Institute Pasteur, Iran).…”

Section: Biosensor Fabricationmentioning

confidence: 99%

Silver Nanoparticles in Various New Applications

Jasni¹,

Ali²,

Sagadevan³

et al. 2021

Silver Micro-Nanoparticles - Properties, Synthesis, Characterization, and Applications

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The use of silver in antimicrobial management is very ancient. Silver-based materials have proven interesting, practical, and promising for various applications. Silver nanoparticles (AgNPs) have been one of the nanostructures most studied and investigated over the past several years. AgNPs have greater specific properties depending on their size and form. These noble synthesised metrics have numerous optical, electrical, catalytic, and optical characteristics. These properties are ideal for many fields, depending on their size and shape. The outbreak of multiple infectious diseases has been a major strain on global economies and the public health sector. Extensive treatments have been suggested for disease control in environments containing infectious diseases through advanced disinfectant nanomaterials. This chapter investigates the application and mechanism of silver nanoparticles in certain nanobiotechnology sectors as a useful nanomaterial. In the sense of the market statistical survey research, AgNPs are emerging as one of the fastest developing product groups in the nanotechnology industry, providing a wide variety of nanosilver products in various applications. Lastly, due to the massive use of AgNPs in products recently, there are many concerns about AgNPs toxicity and safety had also been discussed.

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Section: Biosensor Fabricationmentioning

confidence: 99%

Silver Nanoparticles in Various New Applications

Jasni¹,

Ali²,

Sagadevan³

et al. 2021

Silver Micro-Nanoparticles - Properties, Synthesis, Characterization, and Applications

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“…Refractive index sensitivity was measured using the LSPR biosensing technique. This biosensor was able to detect endotoxins selectively when present with other biological species [67]. 4-mercaptophenylboronic acid (MPBA) functionalized silver nanoparticles (AgNPs) have been used utilized for a novel colorimetric detection of Gram-negative bacteria [68].…”

Section: Silver-based Biosensorsmentioning

confidence: 99%

Nanomaterials for Biosensing Lipopolysaccharide

2019

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Lipopolysaccharides (LPS) are endotoxins, hazardous and toxic inflammatory stimulators released from the outer membrane of Gram-negative bacteria, and are the major cause of septic shock giving rise to millions of fatal illnesses worldwide. There is an urgent need to identify and detect these molecules selectively and rapidly. Pathogen detection has been done by traditional as well as biosensor-based methods. Nanomaterial based biosensors can assist in achieving these goals and have tremendous potential. The biosensing techniques developed are low-cost, easy to operate, and give a fast response. Due to extremely small size, large surface area, and scope for surface modification, nanomaterials have been used to target various biomolecules, including LPS. The sensing mechanism can be quite complex and involves the transformation of chemical interactions into amplified physical signals. Many different sorts of nanomaterials such as metal nanomaterials, magnetic nanomaterials, quantum dots, and others have been used for biosensing of LPS and have shown attractive results. This review considers the recent developments in the application of nanomaterials in sensing of LPS with emphasis given mainly to electrochemical and optical sensing.

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“…Localized surface plasmon resonance (LSPR) is a phenomenon in which light resonates with the collective oscillation of free electrons on the surface of nanoparticles when they enter the noble metal nanoparticles smaller than the wavelength of the incident light 10 . LSPR biosensors that depend on the position or intensity of the resonance according to the refractive index around the nanoparticles have been widely studied with advantages such as a fairly simple method, high sensitivity, and real-time detection 11 , 12 . Generally, the biomolecules contained in the buffer solution have a larger refractive index than the buffer solution, and their adsorption or dissociation onto the noble metal nanoparticles causes a refractive index change around the nanoparticles 13 .…”

Section: Introductionmentioning

confidence: 99%

Design and validation of fiber optic localized surface plasmon resonance sensor for thyroglobulin immunoassay with high sensitivity and rapid detection

Kim

Jeong

Lee

et al. 2021

Sci Rep

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A simple optical fiber sensor based on localized surface plasmon resonance was constructed for direct and rapid measurement of thyroglobulin (Tg). Specific tests for Tg in patients that have undergone thyroidectomy are limited because of insufficient sensitivity, complicated procedures, and in some cases, a long time to yield a result. A sensitive, fast, and simple method is necessary to relieve the psychological and physical burden of the patient. Various concentrations of Tg were measured in a microfluidic channel using an optical fiber sensor with gold nanoparticles. The sensor chip has a detection limit of 93.11 fg/mL with no specificity for other antigens. The potential applicability of the Tg sensing system was evaluated using arbitrary samples containing specific concentrations of Tg. Finally, the sensor can be employed to detect Tg in the patient’s serum, with a good correlation when compared with the commercial kit.

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Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns

Cited by 37 publications

References 32 publications

Silver Nanoparticles in Various New Applications

Silver Nanoparticles in Various New Applications

Nanomaterials for Biosensing Lipopolysaccharide

Design and validation of fiber optic localized surface plasmon resonance sensor for thyroglobulin immunoassay with high sensitivity and rapid detection

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