Lab-on-fiber optofluidic platform for in situ monitoring of drug release from therapeutic eluting polyelectrolyte multilayers

Abstract

A lab-on-fiber (LOF) optofluidic platform that provides physiologically relevant microenvironment was developed by integrating a long period grating (LPG) coupled with high order cladding mode to achieve high index sensitivity and a liquid-tight capillary tube assembly as a microfluidic chamber for LPG to mimic physiologically relevant microenvironment. We demonstrate the utility of LOF for in-situ monitoring the construction of the [chitosan (CHI)/poly (acrylic acid) (PAA)/gentamicin sulfate (GS)/PAA] n multilayers at monolayer resolution as well as evaluating the rate of GS release at a flow rate of 0.127 mL/min at 37 °C in real time. We reveal that GS is released at a faster rate under the dynamic flow condition than in a static medium. Our findings underscore the importance of conducting drug release studies in physiologically relevant conditions.

Optofluidics in Microstructured Optical Fibers

“…To achieve optofluidics in MOFs, regardless of which type of fiber is employed, it is technically challenging to fill the aqueous solution into the air holes of MOFs properly, especially for solutions with high viscosity. There have been some excellent techniques reported to enable liquids to flow into the air holes of MOFs [ 2 , 4 , 15 , 17 , 28 , 29 , 32 , 33 , 34 ]. The initial and easy way is to butt-couple light into the MOFs, leaving a gap between the SMF (single mode fiber) and MOFs so that fluids can enter the air holes [ 2 ].…”

Role of silica nanoparticles in monitoring and prolonging release of drug-eluting polyelectrolyte coatings using long-period fiber grating platform

“…The GS release profiles were obtained in situ by tracking time-dependent resonance wavelength shift in light transmission in LPG using a super-K ultra broadband supercontinuum light source and optical spectrum analyzer (OSA, Anritsu MS9710 C). More details can be found in ref [35].…”

“…[CHI/PAA/GS/PAA]n tetralayer building blocks for LbL coatings satisfy the above requirements, as demonstrated by its effectiveness in treating bacterial infection in animal studies [18,39,40]. Briefly, GS begins to release immediately upon immersion with an initial burst drug elution, followed by a prolonged release at a much slower rate [35]. The initial burst release is due to the excess amount of GS diffusion out from the thin film, reduction of the charge state of GS by the increase of the pH from 5 to 7.4, and the salts of the PBS buffer diffuse into the thin film to compete with the ionic sites of GS [35,42].…”

An ultra wideband-high spatial resolution-compact electric field sensor based on Lab-on-Fiber technology

“…The idea underlying LOF technology is to transform a standard optical fiber into a compact sensor by integrating at the optical fiber (typically the apex) functionnalized materials and/or optically sensitive components of nanometric dimensions (the lab part) 21–24 . LOF has been successfully used for instance as a radiation dosimeter for ultra-high dose monitoring 25 , as a label-free chemical and biological sensor 26 , and as in situ monitoring of drug release 27 .…”