Lab-on-a-chip based biosensor for the real-time detection of aflatoxin

Uludag, Yildiz; Esen, Elif; Kokturk, Guzin; Özer, Hayrettin; Muhammad, Turghun; Ölçer, Zehra; Basegmez, Hatice Imge Oktay; Şimşek, Şenay; Barut, Serkan; Gok, M.; Akgün, Mete; Altıntaş, Zeynep

doi:10.1016/j.talanta.2016.07.060

Cited by 43 publications

(18 citation statements)

References 31 publications

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“…Given this, despite SPR sensors being optical sensors, we have compiled them in this section. Also, the biosensors like lab on chip [344], microfluidic [345], colorimetric, piezoelectric or quartz crystal microbalance (QCM) [346] and resonance light scattering (RLS)-based sensors are still under-developed. In spite of the versatility of piezoelectric sensors, no GQDs-based QCM sensor has been reported so far.…”

Section: Photoelectrochemical and Miscellaneous Gqd Sensors In Biomedmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

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164

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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Section: Photoelectrochemical and Miscellaneous Gqd Sensors In Biomedmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

Self Cite

164

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“…Additionally, a microfluidic immunochip was developed by Arévalo et al [ 105 ] for the detection of CIT in rice by electrochemical detection; CIT-ovalbumin (OVA) conjugates were immobilized on gold disk modified with cysteamine, the LOD of 0.1 μg/L and LOQ of 0.5 μg/L for CIT. Furthermore, the microfluidic chip coupled with gold microelectrode arrays was developed by Olcer [ 106 ] and Uludag [ 107 ] for real-time electrochemical detection of DON in wheat with a LOD of 6.25 μg/L and AFB1 in foods with a LOD of 0.08–0.65 μg/kg ( Figure 4 ). The novel biochips consisted of six working electrodes with shared references and counter electrodes, and the microfluidic channel was created on the electrode arrays by double-sided sticky tape.…”

Section: Application Of Microchips For the Determination Of Mycotomentioning

confidence: 99%

Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip

Man

Liang

Liu

et al. 2017

Toxins

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Mycotoxins are one of the main factors impacting food safety. Mycotoxin contamination has threatened the health of humans and animals. Conventional methods for the detection of mycotoxins are gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS), or enzyme-linked immunosorbent assay (ELISA). However, all these methods are time-consuming, require large-scale instruments and skilled technicians, and consume large amounts of hazardous regents and solvents. Interestingly, a microchip requires less sample consumption and short analysis time, and can realize the integration, miniaturization, and high-throughput detection of the samples. Hence, the application of a microchip for the detection of mycotoxins can make up for the deficiency of the conventional detection methods. This review focuses on the application of a microchip to detect mycotoxins in foods. The toxicities of mycotoxins and the materials of the microchip are firstly summarized in turn. Then the application of a microchip that integrates various kinds of detection methods (optical, electrochemical, photo-electrochemical, and label-free detection) to detect mycotoxins is reviewed in detail. Finally, challenges and future research directions in the development of a microchip to detect mycotoxins are previewed.

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“…AFB1 production and pollution can occur during all processes along the food chain. Because AFB1 results in significant health and economic problems in many countries, AFB1 contamination is one of the most serious problems threatening food safety (Uludag et al, 2016;Xue et al, 2019). Therefore, it is necessary to exploit novel, low-cost, and fast on-site detection technology as well as miniaturized instruments for real-time monitoring of AFB1 and prevention of AFB1 contamination.…”

Section: Introductionmentioning

confidence: 99%

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Wang

Yang

2020

Front. Microbiol.

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Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the highest toxicity and is labeled as a group I carcinogen in humans and animals. Consequently, the proper control of AFB1 produced from biofilms in food and feed has long been recognized. Moreover, many biosensors have been applied to monitor AFB1 in biofilms in food. Additionally, in recent years, novel molecular recognition elements and transducer elements have been introduced for the detection of AFB1. This review presents an outline of recent progress made in the development of biosensors capable of determining AFB1 in biofilms, such as aptasensors, immunosensors, and molecularly imprinted polymer (MIP) biosensors. In addition, the current feasibility, shortcomings, and future challenges of AFB1 determination and analysis are addressed.

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Lab-on-a-chip based biosensor for the real-time detection of aflatoxin

Cited by 43 publications

References 31 publications

Applications of Graphene Quantum Dots in Biomedical Sensors

Applications of Graphene Quantum Dots in Biomedical Sensors

Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

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