La leishmaniose canine à Leishmania Infantum : actualités épidémiologiques – applications

Bourdoiseau, G.

doi:10.4267/2042/56543

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…The Mediterranean Basin, including Algeria, is an endemic area for visceral leishmaniasis, and dogs constitute a potential reservoir for humans (Bourdoiseau, 2015). The geographical position and the climate of the district of Bouira maintain the cycle of L. infantum.…”

Section: Discussionmentioning

confidence: 99%

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EVALUATION OF COMMERCIAL ELISA, INDIRECT IMMUNOFLUORESCENCE TEST AND qPCR FOR THE DIAGNOSIS OF LEISHMANIA INFANTUM IN ASYMPTOMATIC DOGS FROM BOUIRA, NORTHEAST ALGERIA

2021

Veterinaria

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Serological and molecular tests are important tools to determine and control Canine visceral leishmaniasis (CanL). The absence of pathognomonic symptoms and asymptomatic dogs make clinical diagnosis difficult. This study aims to determine the best combination for the diagnosis of Leishmania infantum in asymptomatic dogs. Ninety-four blood samples were collected from dogs in the province of Bouira, in Algeria and assessed with IFAT, ELISA, and qPCR. The sensitivity and specificity for each technique were evaluated in comparison with the indirect fluorescent antibody test (IFAT), which is considered the bestsuited test to determine the presence of L. infantum. In addition, the agreement between these tests was assessed. The use of Cohen's Kappa coefficient demonstrated a moderate agreement between IFAT and qPCR (k= 0.475), and fair between the IFAT and ELISA (k=0.297). The sensitivity of the molecular test was higher (55.56%) than that of the ELISA (33.33%). According to those results, it is better to combine two tests for the diagnosis of CanL, especially in asymptomatic dogs, which is generally not included in the CanL control program.

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Section: Discussionmentioning

confidence: 99%

“…Dog constitutes the main reservoir/ host of L. infantum. It is frequently exposed to relapse after clinical recovery, which makes it a veritable target for reinforced and offensive preventive and vaccine prophylaxis, especially in endemic areas (Bourdoiseau, 2015). However, early diagnosis remains one of the means to control the infection.…”

Section: Introductionmentioning

confidence: 99%

EVALUATION OF COMMERCIAL ELISA, INDIRECT IMMUNOFLUORESCENCE TEST AND qPCR FOR THE DIAGNOSIS OF LEISHMANIA INFANTUM IN ASYMPTOMATIC DOGS FROM BOUIRA, NORTHEAST ALGERIA

2021

Veterinaria

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“…The epidemiological role of both clinically and non‐clinically Leishmania infected dogs is very important as they are the main reservoirs of parasites (Bourdoiseau, 2015 ). Clinical signs are highly polymorphic and include general signs (weight loss, lethargy and anaemia) and specific involvements (lesions in skin, kidney and eye tissues) (Gharbi et al., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis

Goulli

Zribi

Sanhaji

et al. 2022

Veterinary Medicine & Sci

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Background Ocular manifestations in dogs with leishmaniasis are frequent and complications in affected tissues can lead to blindness. Immune processes play a very important role in the pathogenesis of ocular inflammation. Therefore, the immunology of ocular manifestations in dogs with leishmaniasis remains complex and poorly understood. Objectives Estimation and characterisation of ocular and periocular manifestations in dogs naturally infected with Leishmania infantum and investigation of the production site of specific anti‐Leishmania infantum IgG. Methods The present investigation used 53 confirmed dogs infected with Leishmania infantum, presenting ocular and periocular lesions, and 10 control non‐infected dogs. Complete macroscopic ophthalmic examination of eyelids and globes was performed. Both total and anti‐Leishmania infantum IgG antibodies were studied in sera and aqueous humour (AH) of all dogs by ELISA technique. A Goldmann–Witmer coefficient (C value) was calculated. Results The main ophthalmological findings were keratoconjunctivitis (71.7%; 38/53), hyperplasia of conjunctival lymphoid follicles (54.7%; 29/53), blepharitis (50.9%; 27/53) and uveitis (20.7%; 11/53). Ocular production of anti‐Leishmania infantum IgG was detected in 73.6% (39/53) of infected dogs. There was no correlation between the antibody levels in AH and sera of the same dog. The mean anti‐Leishmania infantum IgG in AH was higher in uveitis, followed by lesions affecting only the adnexa (p < 0.0001). The highest mean C values were observed for uveitis, conjunctivitis and keratitis. Conclusions Our findings suggest that production of anti‐Leishmania IgG in dogs infected with Leishmania infantum with ocular manifestations begin in situ and follows by a transfer of antibodies from the bloodstream to the AH.

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La leishmaniose canine à Leishmania Infantum : actualités épidémiologiques – applications

Cited by 2 publications

References 18 publications

EVALUATION OF COMMERCIAL ELISA, INDIRECT IMMUNOFLUORESCENCE TEST AND qPCR FOR THE DIAGNOSIS OF LEISHMANIA INFANTUM IN ASYMPTOMATIC DOGS FROM BOUIRA, NORTHEAST ALGERIA

EVALUATION OF COMMERCIAL ELISA, INDIRECT IMMUNOFLUORESCENCE TEST AND qPCR FOR THE DIAGNOSIS OF LEISHMANIA INFANTUM IN ASYMPTOMATIC DOGS FROM BOUIRA, NORTHEAST ALGERIA

Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis

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