L-Tyrosine Accumulation by Phenylalanine-less Mutant of Glutamic Acid Producing Bacteria

Nakayama, Kiyoshi; Sato, Zenroku; Kinoshita, Shukuo

doi:10.1271/nogeikagaku1924.35.2_146

Cited by 2 publications

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“…In this paper, the results of the experiments on the isomerization of phenylalanine are presented. The formation and accumulation of L-phenylalanine were observed in fermentations with Bacillus strains by Shibuya et al (1960), with Escherichia coli by Huang (1961), and with Micrococcus glutamicus by Nakayama, Sato, and Kinoshita (1961). Asai, Aida, and Oishi (1960) and Kitai, Kitamura, and Miyachi (1962) independently reported enzymatic conversion of phenylpyruvic acid to L-phenylalanine with microbial transaminase.…”

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confidence: 97%

Amino Acid Isomerization in the Production of l-Phenylalanine from d-Phenylalanine by Bacteria1

Chibata¹,

Tosa²,

Sano³

1965

Applied Microbiology

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CHIBATA, ICHIRO (Tanabe Seiyaku Co., Ltd., Osaka, Japan), TETSUYA ToSA, AND RYUJIRO SANO. Amino acid isomerization in the production of L-phenylalanine from D-phenylalanine by bacteria. Appl. Microbiol. 13:618-624. 1965.-To establish an advantageous method for the production of L-amino acids, microbial isomerization of D-and DL-amino acids to L-amino acids was studied. Screening experiments on a number of microorganisms showed that cell suspensions of Pseudomonas fluorescens and P. miyamizu were capable of isomerizing D-and DL-phenylalanines to L-phenylalanine. Various conditions suitable for isomerization by these organisms were investigated. Cells grown in a medium containing D-phenylalanine showed highest isomerization activity, and almost completely converted Dor DL-phenylalanine into L-phenylalanine within 24 to 48 hr of incubation. Enzymatic studies on this isomerizing system suggested that the isomerization of Dor DL-phenylalanine is not catalyzed by a single enzyme, (Camino acid isomerase," but the conversion proceeds by a two step system as follows: D-pheylalanine is oxidized to phenylpyruvic acid by D-amino acid oxidase, and the acid is converted to L-phenylalanine by transamination or reductive amination.

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confidence: 97%